COMPARISON OF CUTANEOUS AND IN VITRO HUMAN MAST CELL HISTAMINE RELEASE BY MUSCLE RELAXANTS

The intravenous injection of vancomycin sometimes causes anaphylactoid reactions, in which histamine release may play a major role. These reactions are more frequently manifested when vancomycin is injected into anesthetized patients. We examined the vancomycin-induced histamine release and the interaction of vancomycin with muscle relaxants or opioid in rats. In an in vitro study with rat peritoneal mast cells, treatment with vancomycin at concentrations of greater than 1.25 mM produced significant histamine release. Tubocurarine, vecuronium, pancuronium, succinylcholine, and morphine up to concentrations of 0.25, 1, 5, 30, and 5 mM, respectively, produced no significant histamine release. However, the nonsignificant histamine release induced by 0.5 mM vancomycin was clearly enhanced by combining vancomycin with any of these agents. In the in vivo study, the intravenous injection of vancomycin significantly increased the plasma histamine levels in rats when vancomycin was injected at 200 mg/kg of body weight (63.2 ± 34.0 ng/ml [mean ± standard deviation]) but not when it was injected at 100 mg/kg (30.8 ± 20.2 ng/ml) compared with that in the saline-treated rats (22.5 ± 11.4 ng/ml). Although the subcutaneous administration of morphine (10 mg/kg) never increased the plasma histamine levels, the intravenous injection of vancomycin (100 mg/kg) 30 min after this morphine treatment markedly increased the plasma histamine levels (56.0 ± 26.9 ng/ml). These findings provide experimental evidence that the combination of muscle relaxants or an opioid with vancomycin may increase the risk of anaphylactoid reactions by enhancing the release of histamine.

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Miltefosine Inhibits Human Mast Cell Activation and Mediator Release Both In Vitro and In Vivo

Journal of Investigative Dermatology ◽

10.1038/jid.2008.248 ◽

2009 ◽

Vol 129 (2) ◽

pp. 496-498 ◽

Cited By ~ 19

Author(s):

Karsten Weller ◽

Metin Artuc ◽

Gary Jennings ◽

Tim Friedrichson ◽

Sven Guhl ◽

...

Keyword(s):

Mast Cell ◽

Cell Activation ◽

Mediator Release ◽

Mast Cell Activation ◽

Human Mast Cell

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Inhibitory Effects of BiRyuChe-Bang on Mast Cell-Mediated Allergic Reactions and Inflammatory Cytokines Production

The American Journal of Chinese Medicine ◽

10.1142/s0192415x13500857 ◽

2013 ◽

Vol 41 (06) ◽

pp. 1267-1282 ◽

Cited By ~ 14

Author(s):

Phil-Dong Moon ◽

Il Sang Choi ◽

Ji-Hyun Go ◽

Byong-Joo Lee ◽

Sang Woo Kang ◽

...

Keyword(s):

Mast Cell ◽

Mast Cells ◽

Histamine Release ◽

Inflammatory Cytokines ◽

Medical Center ◽

Human Mast Cell ◽

Enzyme Linked Immunosorbent Assay ◽

Allergic Reactions ◽

Linalyl Acetate ◽

Tnf Α

BiRyuChe-bang (BRC) is a Korean prescription medicine, which has been used to treat allergic rhinitis at Kyung Hee Medical Center. In this work, we investigated the effects of BRC on mast cell-mediated allergic reactions and inflammatory cytokines production, and identified the active component of BRC. Histamine release was measured from rat peritoneal mast cells (RPMCs). Ear swelling and passive cutaneous anaphylaxis (PCA) were examined in mouse models. Phorbol 12-myristate 13-acetate (PMA) plus A23187-induced inflammatory cytokines production was measured using enzyme-linked immunosorbent assay. Reverse transcriptase-polymerase chain reaction was used for the expressions of tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-8. Activation of nuclear factor (NF)-κB was analyzed by Western blotting. BRC significantly inhibited the compound 48/80-induced ear swelling response, histamine release from RPMCs, PCA activated by anti-dinitrophenyl IgE, and PMA plus A23187-induced inflammatory cytokines production (p < 0.05). In addition, BRC dose-dependently inhibited the mRNA expressions of TNF-α, IL-6, and IL-8 as well as the activation of NF-κB in a human mast cell line, HMC-1 cells. BRC inhibited the levels of TNF-α and IL-6 in mice induced with PCA. Several components of BRC, such as 1,8-Cineole, Linalool, Linalyl acetate, α-Pinene, and α-Terpineol, significantly inhibited the release of histamine from RPMCs (p < 0.05). Among these components, Linalyl acetate was the most effective for inhibiting histamine release. These results indicate that BRC has a potential regulatory effect on allergic and inflammatory reactions mediated by mast cells.

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Cysteine Protease Activity and Histamine Release from the Human Mast Cell Line HMC-1 Stimulated by Recombinant Streptococcal Pyrogenic Exotoxin B/Streptococcal Cysteine Protease

Infection and Immunity ◽

10.1128/iai.70.7.3944-3947.2002 ◽

2002 ◽

Vol 70 (7) ◽

pp. 3944-3947 ◽

Cited By ~ 19

Author(s):

Yukino Watanabe ◽

Yuko Todome ◽

Hisashi Ohkuni ◽

Shinsaku Sakurada ◽

Toshio Ishikawa ◽

...

Keyword(s):

Mast Cell ◽

Histamine Release ◽

Cell Line ◽

Cysteine Protease ◽

Protease Activity ◽

Human Mast Cell ◽

Streptococcal Toxic Shock Syndrome ◽

Streptococcal Pyrogenic Exotoxin B ◽

Mast Cell Line ◽

Spe B

ABSTRACT We constructed the expression vector pSK-SCP containing the streptococcal exotoxin B gene (spe b) which expressed protease activity. We showed that the recombinant streptococcal pyogenic exotoxin B/streptococcal cysteine protease (rSPE B/SCP) was secreted into the culture supernatant of the transformant and retained its SCP activity, which was equivalent to or greater than that of the naturally occurring molecule. The secreted rSPE B/SCP induced histamine release and degranulation of the human mast cell line HMC-1. This study may contribute to the understanding of the pathogenic role of SPE B/SCP in streptococcal infection and streptococcal toxic shock syndrome.

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