scholarly journals Correction: A Raf/MEK/ERK signaling pathway is required for development of the sea urchin embryo micromere lineage through phosphorylation of the transcription factor Ets

Development ◽  
2004 ◽  
Vol 131 (9) ◽  
pp. 2233-2233
Keyword(s):  
Transcription Factor ◽  
Signaling Pathway ◽  
Sea Urchin ◽  
Erk Signaling ◽  
Sea Urchin Embryo

Spatially regulated SpEts4 transcription factor activity along the sea urchin embryo animal-vegetal axis

Development ◽  
1999 ◽  
Vol 126 (8) ◽  
pp. 1729-1737 ◽  
Author(s):  
Z. Wei ◽  
L.M. Angerer ◽  
R.C. Angerer
Keyword(s):  
Transcription Factor ◽  
Sea Urchin ◽  
Regulatory Region ◽  
High Specificity ◽  
Cis Element ◽  
Sea Urchin Embryo ◽  
Transcriptional Regulatory ◽  
Maternal Transcripts ◽  
Turn Over

Because the transcription of the SpHE gene is regulated cell-autonomously and asymmetrically along the maternally determined animal-vegetal axis of the very early sea urchin embryo, its regulators provide an excellent entry point for investigating the mechanism(s) that establishes this initial polarity. Previous studies support a model in which spatial regulation of SpHE transcription relies on multiple nonvegetal positive transcription factor activities (Wei, Z., Angerer, L. M. and Angerer, R. C. (1997) Dev. Biol. 187, 71–78) and a yeast one-hybrid screen has identified one, SpEts4, which binds with high specificity to a cis element in the SpHE regulatory region and confers positive activation of SpHE promoter transgenes (Wei, Z., Angerer, R. C. and Angerer, L. M. (1999) Mol. Cell. Biol. 19, 1271–1278). Here we demonstrate that SpEts4 can bind to the regulatory region of the endogenous SpHE gene because a dominant repressor, created by fusing SpEts4 DNA binding and Drosophila engrailed repression domains, suppresses its transcription. The pattern of expression of the SpEts4 gene is consistent with a role in regulating SpHE transcription in the nonvegetal region of the embryo during late cleavage/early blastula stages. Although maternal transcripts are uniformly distributed in the egg and early cleaving embryo, they rapidly turn over and are replaced by zygotic transcripts that accumulate in a pattern congruent with SpHE transcription. In addition, in vivo functional tests show that the SpEts4 cis element confers nonvegetal transcription of a beta-galactosidase reporter gene containing the SpHE basal promoter, and provide strong evidence that the activity of this transcription factor is an integral component of the nonvegetal transcriptional regulatory apparatus, which is proximal to, or part of, the mechanism that establishes the animal-vegetal axis of the sea urchin embryo.

scholarly journals An Elk transcription factor is required for Runx-dependent survival signaling in the sea urchin embryo

2016 ◽  
Vol 416 (1) ◽  
pp. 173-186 ◽  
Author(s):  
Francesca Rizzo ◽  
James A. Coffman ◽  
Maria Ina Arnone
Keyword(s):  
Transcription Factor ◽  
Sea Urchin ◽  
Sea Urchin Embryo ◽  
Survival Signaling

scholarly journals HpEts, an ets-related transcription factor implicated in primary mesenchyme cell differentiation in the sea urchin embryo

1999 ◽  
Vol 80 (1) ◽  
pp. 41-52 ◽  
Author(s):  
Daisuke Kurokawa ◽  
Takashi Kitajima ◽  
Keiko Mitsunaga-Nakatsubo ◽  
Shonan Amemiya ◽  
Hiraku Shimada ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Cell Differentiation ◽  
Sea Urchin ◽  
Sea Urchin Embryo ◽  
Mesenchyme Cell ◽  
Related Transcription Factor

Hepatocyte growth factor/scatter factor activates the ETS1 transcription factor by a RAS-RAF-MEK-ERK signaling pathway

Oncogene ◽  
2002 ◽  
Vol 21 (15) ◽  
pp. 2309-2319 ◽  
Author(s):  
Rejane Paumelle ◽  
David Tulashe ◽  
Zoulika Kherrouche ◽  
Serge Plaza ◽  
Catherine Leroy ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Signaling Pathway ◽  
Erk Signaling ◽  
Scatter Factor ◽  
Hepatocyte Growth

SpSoxB1, a maternally encoded transcription factor asymmetrically distributed among early sea urchin blastomeres

Development ◽  
1999 ◽  
Vol 126 (23) ◽  
pp. 5473-5483 ◽  
Author(s):  
A.P. Kenny ◽  
D. Kozlowski ◽  
D.W. Oleksyn ◽  
L.M. Angerer ◽  
R.C. Angerer
Keyword(s):  
Transcription Factor ◽  
Sea Urchin ◽  
Protein Distribution ◽  
Blastula Stage ◽  
Cell Stage ◽  
Mrna Accumulation ◽  
Cis Element ◽  
Sea Urchin Embryo ◽  
Asymmetrically Distributed

We have identified a Sox family transcription factor, SpSoxB1, that is asymmetrically distributed among blastomeres of the sea urchin embryo during cleavage, beginning at 4th cleavage. SpSoxB1 interacts with a cis element that is essential for transcription of SpAN, a gene that is activated cell autonomously and expressed asymmetrically along the animal-vegetal axis. In vitro translated SpSoxB1 forms a specific complex with this cis element whose mobility is identical to that formed by a protein in nuclear extracts. An anti-SpSoxB1 rabbit polyclonal antiserum specifically supershifts this DNA-protein complex and recognizes a single protein on immunoblots of nuclear proteins that comigrates with in vitro translated SpSoxB1. Developmental immunoblots of total proteins at selected early developmental stages, as well as EMSA of egg and 16-cell stage proteins, show that SpSoxB1 is present at low levels in unfertilized eggs and progressively accumulates during cleavage. SpSoxB1 maternal transcripts are uniformly distributed in the unfertilized egg and the protein accumulates to similar, high concentrations in all nuclei of 4- and 8-cell embryos. However, at fourth cleavage, the micromeres, which are partitioned by asymmetric division of the vegetal 4 blastomeres, have reduced nuclear levels of the protein, while high levels persist in their sister macromeres and in the mesomeres. During cleavage, the uniform maternal SpSoxB1 transcript distribution is replaced by a zygotic nonvegetal pattern that reinforces the asymmetric SpSoxB1 protein distribution and reflects the corresponding domain of SpAN mRNA accumulation at early blastula stage (approximately 150 cells). The vegetal region lacking nuclear SpSoxB1 gradually expands so that, after blastula stage, only cells in differentiating ectoderm accumulate this protein in their nuclei. The results reported here support a model in which SpSoxB1 is a major regulator of the initial phase of asymmetric transcription of SpAN in the nonvegetal domain by virtue of its distribution at 4th cleavage and is subsequently an important spatial determinant of expression in the early blastula. This factor is the earliest known spatially restricted regulator of transcription along the animal-vegetal axis of the sea urchin embryo.

The activity of the TGFβ-inducible transcription factor TIEG2 is antagonized by the proliferative EGFR-MEK-ERK signaling pathway

2001 ◽  
Vol 120 (5) ◽  
pp. A497-A497
Author(s):  
V ELLENRIEDER ◽  
J ZHANG ◽  
J KACZYNSKI ◽  
R URRUTIA
Keyword(s):  
Transcription Factor ◽  
Signaling Pathway ◽  
Erk Signaling

scholarly journals Role of the ERK-mediated signaling pathway in mesenchyme formation and differentiation in the sea urchin embryo

2004 ◽  
Vol 268 (2) ◽  
pp. 384-402 ◽  
Author(s):  
Montserrat Fernandez-Serra ◽  
Claudia Consales ◽  
Alessandra Livigni ◽  
Maria I Arnone
Keyword(s):  
Signaling Pathway ◽  
Sea Urchin ◽  
Sea Urchin Embryo

scholarly journals Erratum: Hepatocyte growth factor/scatter factor activates the ETS1 transcription factor by a RAS-RAF-MEK-ERK signaling pathway

Oncogene ◽  
2002 ◽  
Vol 21 (31) ◽  
pp. 4872-4872
Author(s):  
Rejane Paumelle ◽  
David Tulasne ◽  
Zoulika Kherrouche ◽  
Serge Plaza ◽  
Catherine Leroy ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Signaling Pathway ◽  
Erk Signaling ◽  
Scatter Factor ◽  
Hepatocyte Growth
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