scholarly journals Molecular signatures identify immature mesenchymal progenitors in early mouse limb buds that respond differentially to morphogen signaling

Development ◽  
2019 ◽  
Vol 146 (10) ◽  
pp. dev173328 ◽  
Author(s):  
Robert Reinhardt ◽  
Fabiana Gullotta ◽  
Gretel Nusspaumer ◽  
Erkan Ünal ◽  
Robert Ivanek ◽  
...  
Keyword(s):  
Molecular Signatures ◽  
Limb Buds ◽  
Mesenchymal Progenitors ◽  
Mouse Limb ◽  
Early Mouse

scholarly journals SMAD4 target genes are part of a transcriptional network that integrates the response to BMP and SHH signaling during early limb bud patterning

Development ◽  
2021 ◽  
Author(s):  
Julie Gamart ◽  
Iros Barozzi ◽  
Frédéric Laurent ◽  
Robert Reinhardt ◽  
Laurène Ramos Martins ◽  
...  
Keyword(s):  
Gene Expression ◽  
Signal Transduction ◽  
Target Genes ◽  
Cholesterol Biosynthesis ◽  
Limb Bud ◽  
Limb Buds ◽  
Shh Signaling ◽  
Mesenchymal Progenitors ◽  
Repressive Effect ◽  
Mouse Limb

SMAD4 regulates gene expression in response to BMP and TGFβ signal transduction and is required for diverse morphogenetic processes, but its target genes have remained largely elusive. Here, we identify the SMAD4 target genes in mouse limb buds using an epitope-tagged Smad4 allele for ChIP-seq analysis in combination with transcription profiling. This analysis shows that SMAD4 predominantly mediates BMP signal-transduction during early limb bud development. Unexpectedly, the expression of cholesterol biosynthesis enzymes is precociously down-regulated and intracellular cholesterol levels are reduced in Smad4-deficient limb bud mesenchymal progenitors. Most importantly, our analysis reveals a predominant function of SMAD4 in up-regulating target genes in the anterior limb bud mesenchyme. Analysis of differentially expressed genes shared between Smad4- and Shh-deficient limb buds corroborates this function of SMAD4 and also reveals the repressive effect of SMAD4 on posterior genes that are up-regulated in response to SHH signaling. This analysis uncovers opposing trans-regulatory inputs from SHH and SMAD4-mediated BMP signal transduction on anterior and posterior gene expression during the digit patterning and outgrowth in early limb buds.

Chlorambucil-induced cell death in embryonic mouse limb buds

1976 ◽  
Vol 37 (2) ◽  
pp. 237-256 ◽  
Author(s):  
T.W. Sadler ◽  
D.M. Kochhar
Keyword(s):  
Cell Death ◽  
Limb Buds ◽  
Embryonic Mouse ◽  
Mouse Limb

Dual requirement of ectodermalSmad4during AER formation and termination of feedback signaling in mouse limb buds

genesis ◽  
2013 ◽  
pp. 1-7
Author(s):  
Jean-Denis Benazet ◽  
Rolf Zeller
Keyword(s):  
Limb Buds ◽  
Mouse Limb

Lectin binding sites in developing mouse limb buds

1991 ◽  
Vol 184 (5) ◽  
pp. 479-488 ◽  
Author(s):  
J. Milaire
Keyword(s):  
Binding Sites ◽  
Lectin Binding ◽  
Limb Buds ◽  
Mouse Limb ◽  
Lectin Binding Sites

Effect of Water Source on the Growth and Development of CD-1 Mouse Limb Buds in Submerged Culture

1992 ◽  
Vol 2 (3) ◽  
pp. 177-187 ◽  
Author(s):  
C. G. Rousseaux ◽  
B. E. Thompson ◽  
B. Zintel
Keyword(s):  
Growth And Development ◽  
Submerged Culture ◽  
Water Source ◽  
Limb Buds ◽  
Effect Of Water ◽  
Mouse Limb

scholarly journals Conserved cis-regulatory regions in a large genomic landscape control SHH and BMP-regulated Gremlin1 expression in mouse limb buds

2012 ◽  
Vol 12 (1) ◽  
pp. 23 ◽  
Author(s):  
Aimée Zuniga ◽  
Frédéric Laurent ◽  
Javier Lopez-Rios ◽  
Christian Klasen ◽  
Nicolas Matt ◽  
...  
Keyword(s):  
Limb Buds ◽  
Regulatory Regions ◽  
Genomic Landscape ◽  
Mouse Limb ◽  
Landscape Control

Induction of skeletal malformations in organ cultures of mouse limb buds

1975 ◽  
Vol 31 (2) ◽  
pp. 227-228 ◽  
Author(s):  
B. Zimmermann ◽  
D. Neubert ◽  
D. Bachmann ◽  
H. -J. Merker
Keyword(s):  
Organ Cultures ◽  
Limb Buds ◽  
Skeletal Malformations ◽  
Mouse Limb

Activity of Nuclear DNA-Dependent RNA Polymerases in Mouse Limb Buds Differentiating in vivo or in Organ Culture

1976 ◽  
Vol 357 (2) ◽  
pp. 1623-1636 ◽  
Author(s):  
Diether NEUBERT ◽  
Margret RAUTENBERG
Keyword(s):  
Organ Culture ◽  
Nuclear Dna ◽  
Rna Polymerases ◽  
Limb Buds ◽  
Mouse Limb
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