Inhibition of nuclear migration in the absence of microtubules in the chick embryo

Development ◽  
1973 ◽  
Vol 30 (3) ◽  
pp. 661-671
Author(s):  
Paul-Emil Messier ◽  
C. Auclair
Keyword(s):  
Chick Embryo ◽  
New Media ◽  
Neural Tube ◽  
Nuclear Migration ◽  
Chick Embryos ◽  
Interkinetic Nuclear Migration ◽  
In Cells

Interkinetic nuclear migration was studied in cells of the forming neural tube of cultured chick embryos aged from 1 to 7 pairs of somites. In specimens that were first exposed to 2°C for 3 h, then treated with 4 × 10−3 M monoiodoacetamide and finally transferred to new media to be reincubated for 1−2·5 h, it was found that microtubules were absent and nuclear movements blocked. These findings point to microtubules as essential structures for interkinetic nuclear migration. Their relation to the problem of neurulation is discussed.

Neuralation et migration nucléaire intercinétique chez des embryons de poulet

Development ◽  
1975 ◽  
Vol 34 (2) ◽  
pp. 339-354
Author(s):  
Par Paul-Emil Messier ◽  
C. Auclair
Keyword(s):  
Chick Embryo ◽  
Neural Tube ◽  
Cytochalasin B ◽  
Nuclear Migration ◽  
Inhibitory Effect ◽  
Chick Embryos ◽  
Interkinetic Nuclear Migration ◽  
In Cells

Neurulation and interkinetic nuclear migration in the chick embryo Neurulation and interkinetic nuclear migration were studied in cells of the forming neural tube of chick embryos submitted to a variety of treatments. Our results show that cytochalasin B (5 μ/ml) does not protect microtubules against disruption occurring after 3h at 2°C nor does it prevent their repolymerization once they are cold-disrupted. However, db-cAMP protects microtubules against such cold disruption. We indicate that the inhibitory effect of cytochalasin B on interkinetic nuclear migration cannot be ascribed to an effect on microtubules.

Effects of formamide on neuroepithelial cells and on interkinetic nuclear migration in the chick embryo

Development ◽  
1976 ◽  
Vol 35 (1) ◽  
pp. 197-212
Author(s):  
Paul-Emil Messier
Keyword(s):  
Chick Embryo ◽  
Nuclear Migration ◽  
Detectable Effect ◽  
Chick Embryos ◽  
Young Chick ◽  
Interkinetic Nuclear Migration ◽  
Neuroepithelial Cells ◽  
Cytoplasmic Microtubules ◽  
Cell Asymmetry ◽  
Mitotic Figures

Young chick embryos were incubated on media containing formamide at concentrations of 0·1, 0·25, 0·31, 0·37, 0·43 and 0·5 M. In the neuroepithelium of these embryos we found that (1) the 0·1 M concentration had no detectable effect, (2) the 0·25 M concentration only affected mitosis which was blocked in metaphase so that mitotic figures accumulated near the neurocoele, (3) 0·31 M formamide totally inhibited interkinetic nuclear migration and affected only slightly the cell asymmetry, (4) the 0·37 M concentration considerably reduced the amount of cytoplasmic microtubules and that the cells became round, (5) at 0·43 M formamide, all microtubules had disappeared and all cells were spherical, (6) at 0·5 M formamide all cells were spherical, detached from one another and the epithelium had lost its usual characteristics. Our results on exposure of the cells to low temperature (2 °C) suggest that formamide directly affects microtubules. All the effects observed at concentrations up to 0·43 M formamide are reversible.

scholarly journals Misexpression of BRE gene in the developing chick neural tube affects neurulation and somitogenesis

2015 ◽  
Vol 26 (5) ◽  
pp. 978-992 ◽  
Author(s):  
Guang Wang ◽  
Yan Li ◽  
Xiao-Yu Wang ◽  
Manli Chuai ◽  
John Yeuk-Hon Chan ◽  
...  
Keyword(s):  
Chick Embryo ◽  
Embryonic Development ◽  
Neural Crest ◽  
Embryo Development ◽  
Neural Tube ◽  
Neural Crest Cells ◽  
Chick Embryos ◽  
Early Chick Embryo

This is the first study of the role of BRE in embryonic development using early chick embryos. BRE is expressed in the developing neural tube, neural crest cells, and somites. BRE thus plays an important role in regulating neurogenesis and indirectly somitogenesis during early chick embryo development.

Volumetric Investigations on Overgrowth (Hypermorphosis) in Chick Embryo Brains

Development ◽  
1960 ◽  
Vol 8 (2) ◽  
pp. 69-72
Author(s):  
Harry Bergquist
Keyword(s):  
Chick Embryo ◽  
Neural Tube ◽  
Relevant Literature ◽  
Mitotic Rate ◽  
Chick Embryos ◽  
Tectum Opticum ◽  
Surface Areas ◽  
The Brain

The present paper is intended as a continuation of preceding publications (Bergquist, 1959 a, b) on possible causes of the phenomenon of ‘overgrowth’ (Patten, 1952) in the brain of the chick embryo. Reference should be made to these publications for the relevant literature. In these earlier investigations, it was observed that the mitotic rate in the altered portions of the neural tube seemed higher than in the normal regions, and that the hemispheres and tectum opticum showed marked folding. The first aim of the present investigation was to compare the volumes of the hemispheres in normal chick embryos and in corresponding stages of embryos with overgrowth, the study being confined to the distinctly evaginated portions of both hemispheres. The embryos measured consisted of those used in the previous work. The surface areas of sections through the walls of both hemispheres were determined planimetrically.

Patterns of cell division and interkinetic nuclear migration in the chick embryo hindbrain

1991 ◽  
Vol 22 (7) ◽  
pp. 742-754 ◽  
Author(s):  
Sarah Guthrie ◽  
Matthew Butcher ◽  
Andrew Lumsden
Keyword(s):  
Cell Division ◽  
Chick Embryo ◽  
Nuclear Migration ◽  
Interkinetic Nuclear Migration
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