Effects of formamide on neuroepithelial cells and on interkinetic nuclear migration in the chick embryo

Development ◽  
1976 ◽  
Vol 35 (1) ◽  
pp. 197-212
Author(s):  
Paul-Emil Messier
Keyword(s):  
Chick Embryo ◽  
Nuclear Migration ◽  
Detectable Effect ◽  
Chick Embryos ◽  
Young Chick ◽  
Interkinetic Nuclear Migration ◽  
Neuroepithelial Cells ◽  
Cytoplasmic Microtubules ◽  
Cell Asymmetry ◽  
Mitotic Figures

Young chick embryos were incubated on media containing formamide at concentrations of 0·1, 0·25, 0·31, 0·37, 0·43 and 0·5 M. In the neuroepithelium of these embryos we found that (1) the 0·1 M concentration had no detectable effect, (2) the 0·25 M concentration only affected mitosis which was blocked in metaphase so that mitotic figures accumulated near the neurocoele, (3) 0·31 M formamide totally inhibited interkinetic nuclear migration and affected only slightly the cell asymmetry, (4) the 0·37 M concentration considerably reduced the amount of cytoplasmic microtubules and that the cells became round, (5) at 0·43 M formamide, all microtubules had disappeared and all cells were spherical, (6) at 0·5 M formamide all cells were spherical, detached from one another and the epithelium had lost its usual characteristics. Our results on exposure of the cells to low temperature (2 °C) suggest that formamide directly affects microtubules. All the effects observed at concentrations up to 0·43 M formamide are reversible.

Inhibition of nuclear migration in the absence of microtubules in the chick embryo

Development ◽  
1973 ◽  
Vol 30 (3) ◽  
pp. 661-671
Author(s):  
Paul-Emil Messier ◽  
C. Auclair
Keyword(s):  
Chick Embryo ◽  
New Media ◽  
Neural Tube ◽  
Nuclear Migration ◽  
Chick Embryos ◽  
Interkinetic Nuclear Migration ◽  
In Cells

Interkinetic nuclear migration was studied in cells of the forming neural tube of cultured chick embryos aged from 1 to 7 pairs of somites. In specimens that were first exposed to 2°C for 3 h, then treated with 4 × 10−3 M monoiodoacetamide and finally transferred to new media to be reincubated for 1−2·5 h, it was found that microtubules were absent and nuclear movements blocked. These findings point to microtubules as essential structures for interkinetic nuclear migration. Their relation to the problem of neurulation is discussed.

Neuralation et migration nucléaire intercinétique chez des embryons de poulet

Development ◽  
1975 ◽  
Vol 34 (2) ◽  
pp. 339-354
Author(s):  
Par Paul-Emil Messier ◽  
C. Auclair
Keyword(s):  
Chick Embryo ◽  
Neural Tube ◽  
Cytochalasin B ◽  
Nuclear Migration ◽  
Inhibitory Effect ◽  
Chick Embryos ◽  
Interkinetic Nuclear Migration ◽  
In Cells

Neurulation and interkinetic nuclear migration in the chick embryo Neurulation and interkinetic nuclear migration were studied in cells of the forming neural tube of chick embryos submitted to a variety of treatments. Our results show that cytochalasin B (5 μ/ml) does not protect microtubules against disruption occurring after 3h at 2°C nor does it prevent their repolymerization once they are cold-disrupted. However, db-cAMP protects microtubules against such cold disruption. We indicate that the inhibitory effect of cytochalasin B on interkinetic nuclear migration cannot be ascribed to an effect on microtubules.

An electrosurgical technique for the production of localized tissue ablations in the early chick embryo

Development ◽  
1971 ◽  
Vol 26 (1) ◽  
pp. 21-29
Author(s):  
R. K. Jordan
Keyword(s):  
Chick Embryo ◽  
Low Frequency ◽  
Alternating Current ◽  
Chick Embryos ◽  
Operating Microscope ◽  
Young Chick ◽  
Early Chick Embryo ◽  
Electrolytic Corrosion ◽  
Tungsten Metal

The passage of low-frequency alternating current was found superior to other methods considered for the production of small, discrete, electrolytic ablations in young chick embryos. Active electrodes of tungsten metal less than 5 µm in diameter were prepared by controlled electrolytic corrosion. These gave reproducible, discrete foci of destruction of the required size, with currents less than 2 mA. The identification of destroyed tissue areas was immediately apparent under the operating microscope and confirmed histologically. Preliminary studies on bilateral extirpation of the ultimobranchial primordia show the absence of the ultimobranchial bodies 6 days after destruction of the primordia at 96 h of incubation.

Patterns of cell division and interkinetic nuclear migration in the chick embryo hindbrain

1991 ◽  
Vol 22 (7) ◽  
pp. 742-754 ◽  
Author(s):  
Sarah Guthrie ◽  
Matthew Butcher ◽  
Andrew Lumsden
Keyword(s):  
Cell Division ◽  
Chick Embryo ◽  
Nuclear Migration ◽  
Interkinetic Nuclear Migration

Arginase in young chick embryos

1962 ◽  
Vol 26 (1) ◽  
pp. 175-188 ◽  
Author(s):  
Eva Eliasson
Keyword(s):  
Chick Embryos ◽  
Young Chick

Interkinetic nuclear migration in the tracheal and esophageal epithelia of the mouse embryo: Possible implications for tracheo-esophageal anomalies

2017 ◽  
Vol 58 (2) ◽  
pp. 62-70 ◽  
Author(s):  
Ryo Kaneda ◽  
Yuko Saeki ◽  
Dereje Getachew ◽  
Akihiro Matsumoto ◽  
Motohide Furuya ◽  
...  
Keyword(s):  
Mouse Embryo ◽  
Nuclear Migration ◽  
Interkinetic Nuclear Migration
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