scholarly journals Ionic and total calcium levels in the blood of the European eel (Anguilla anguilla): effects of stanniectomy and hypocalcin replacement therapy

1989 ◽  
Vol 141 (1) ◽  
pp. 177-186 ◽  
Author(s):  
R. G. Hanssen ◽  
F. P. Lafeber ◽  
G. Flik ◽  
S. E. Wendelaar Bonga
Keyword(s):  
Replacement Therapy ◽  
Calcium Concentration ◽  
Anguilla Anguilla ◽  
European Eel ◽  
Total Calcium ◽  
Corpuscles Of Stannius ◽  
Ionic Calcium

Removal of the corpuscles of Stannius (STX) in the freshwater European eel causes a marked increase in the concentrations of blood ionic calcium and protein-bound calcium. The hypercalcaemia peaks 20 days after STX and lasts at least another 20 days. In stanniectomized eels hypocalcin decreased both blood ionic and total calcium concentrations. The reduction of plasma total calcium concentration by hypocalcin is attributed to a reduction in blood ionic calcium concentration. We conclude that hypocalcin regulates blood ionic calcium levels in fish.

REGULATION AND DISTRIBUTION OF PLASMA CALCIUM AND INORGANIC PHOSPHATE IN THE EUROPEAN EEL (ANGUILLA ANGUILLA L.)

1968 ◽  
Vol 42 (1) ◽  
pp. 109-117 ◽  
Author(s):  
D. K. O. CHAN ◽  
I. CHESTER JONES
Keyword(s):  
Inorganic Phosphate ◽  
Calcium Concentration ◽  
Anguilla Anguilla ◽  
European Eel ◽  
Total Calcium ◽  
Corpuscles Of Stannius ◽  
Freshwater Eel ◽  
Ionic Calcium ◽  
Relationship Of ◽  
The Relationship

SUMMARY The amounts of ionized, complexed and protein-bound calcium and total inorganic phosphate were measured in the plasma of the eel. Groups comprised freshwater, seawater and distilled water eels; treatment with corticotrophin (ACTH), cortisol, aldosterone or oestrogen; adrenalectomized and hypophysectomized eels and those with the corpuscles of Stannius removed. In the intact animal, the amounts of ultrafiltrable and ionic calcium and inorganic phosphate in the plasma were very constant despite wide variation in the electrolyte composition of the external medium. In the freshwater eel, 1 week after removal of the corpuscles of Stannius, there was a rise in the level of ultrafiltrable calcium and a decline in that of inorganic phosphate. At 3 weeks after operation, although total calcium concentration remained elevated, ultrafiltrable and ionic calcium concentrations returned to control values. Adrenalectomy and hypophysectomy of the freshwater eel resulted in a decline in total calcium concentration with change in the levels of ultrafiltrable calcium or inorganic phosphate. In the seawater eel, adrenalectomy or removal of the corpuscles of Stannius resulted in an increase in total and ultrafiltrable calcium concentrations. Injection of ACTH, cortisol or aldosterone did not affect calcium concentrations but gave a slight decline in inorganic phosphate levels. Injection of oestrogen increased total calcium concentration without affecting ultrafiltrable or ionic calcium concentrations. The relationship of the amounts of ultrafiltrable calcium and inorganic phosphate to the solubility product [ultrafiltrable calcium] × [phosphate] is discussed and attention drawn to the importance of protein-binding in calcium homeostasis.

Influence of Ambient Calcium Levels on Tanniocalcin Secretion in the European EEL (Anguilla Anguilla)

1992 ◽  
Vol 162 (1) ◽  
pp. 197-208 ◽  
Author(s):  
R.G.J. M. HANSSEN ◽  
N. MAYER-GOSTAN ◽  
G. FLIK ◽  
S.E. WENDELAAR BONGA
Keyword(s):  
Sea Water ◽  
Secretory Granules ◽  
Anguilla Anguilla ◽  
Secretory Activity ◽  
European Eel ◽  
Apparent Effect ◽  
Rapid Reduction ◽  
Corpuscles Of Stannius ◽  
Rapid Accumulation ◽  
Ionic Calcium

The cells of the corpuscles of Stannius appeared to be more active in eels acclimated to sea water than in eels acclimated to fresh water. In acclimated eels, however, total and ionic plasma calcium concentrations and stanniocalcin titers did not differ. This suggests that levels of stanniocalcin in freshwater-acclimated eels are sufficient to maintain normocalcemia in sea water. When freshwateracclimated eels were transferred directly to sea water, total and ionic calcium concentrations in the plasma increased significantly within 24 h but there was no apparent effect on the corpuscles of Stannius within the same time. This suggests that changes in secretory activity of the corpuscles of Stannius do not occur rapidly when they are presented with a hypercalcemic challenge. Conversely, when seawater-acclimated eels were transferred to fresh or distilled water there appeared to be a very rapid reduction in secretory activity in the corpuscles of Stannius, as indicated by the rapid accumulation of secretory granules. These data suggest that stanniocalcin turnover is more rapid in seawater-acclimated eels and that the secretory activity of the corpuscles of Stannius is rapidly reduced when a hypercalcic challenge is removed.

scholarly journals Hypocalcaemic Activity of Trout Hypocalcin and Bovine Parathyroid Hormone in Stanniectomized Eels

1988 ◽  
Vol 140 (1) ◽  
pp. 199-208
Author(s):  
FLORIS P.J. G. LAFEBER ◽  
R.G.J. M. HANSSEN ◽  
S. E. WENDELAAR BONGA
Keyword(s):  
Parathyroid Hormone ◽  
Calcium Level ◽  
Calcium Concentration ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Striking Similarity ◽  
Total Calcium ◽  
Molar Basis ◽  
Ionic Calcium ◽  
Bovine Parathyroid Hormone

Hypocalcin isolated from trout corpuscles of Stannius (CS) and bovine parathyroid hormone (bPTH) induce hypocalcaemia when injected intra-arterially in hypercalcaemic, stanniectomized (STX) eels. On a molar basis, both hormones have almost the same potency. Upon injection of hypocalcin we observed a decrease in blood ionic calcium level which fully accounts for the decrease in total calcium. Injections of bPTH also resulted in a decrease of total and ionic calcium concentrations in the blood. The decrease in ionic calcium level upon injection of bPTH, however, did not fully account for the decrease in total calcium concentration of the blood. Nevertheless, hypocalcin and bPTH showed striking similarity in bioactivity in stanniectomized eels. Some similarity in three-dimensional structure of both hormones may underlie this phenomenon.

FAILURE OF CORPUSCLES OF STANNIUS OF THE EUROPEAN EEL (ANGUILLA ANGUILLA L.) TO PRODUCE CORTICOSTEROIDS IN VITRO

1965 ◽  
Vol 33 (2) ◽  
pp. 319-320 ◽  
Author(s):  
I. CHESTER JONES ◽  
D. K. O. CHAN ◽  
I. W. HENDERSON ◽  
W. MOSLEY ◽  
T. SANDOR ◽  
...  
Keyword(s):  
Anguilla Anguilla ◽  
European Eel ◽  
Corpuscles Of Stannius

PRESSOR ACTIVITY IN EXTRACTS OF THE CORPUSCLES OF STANNIUS FROM THE EUROPEAN EEL (ANGUILLA ANGUILLA L.)

1966 ◽  
Vol 34 (3) ◽  
pp. 393-408 ◽  
Author(s):  
I. CHESTER JONES ◽  
I. W. HENDERSON ◽  
D. K. O. CHAN ◽  
J. C. RANKIN ◽  
W. MOSLEY ◽  
...  
Keyword(s):  
Intravenous Injection ◽  
Renin Angiotensin System ◽  
Anguilla Anguilla ◽  
European Eel ◽  
Silver Eel ◽  
Renin Substrate ◽  
Angiotensin System ◽  
Corpuscles Of Stannius ◽  
Silver Eels ◽  
Pressor Activity

SUMMARY Extracts of corpuscles of Stannius from the silver eel have been shown to contain a substance with a powerful pressor action on intravenous injection into the rat. This material resembles mammalian renin in being non-diffusible through cellophane, heat-labile, and destroyed by acidification to pH 2. The effect in the rat differs, however, from that produced by mammalian renin in being more prolonged, and frequently biphasic. Pressor activity has also been demonstrated in extracts of kidneys from freshwater silver eels. Incubation of kidney extract with mammalian renin-substrate produced an angiotensin-like pressor substance. Both renal and corpuscular extracts had a prolonged pressor effect on intravenous injection into the eel. The identities of these pressor materials have not been finally established. Removal of the corpuscles of Stannius from freshwater silver eels was followed by a drop in blood pressure to levels normally found in eels adapted to seawater. The possible existence, in the eel, of a renin-angiotensin system analogous to that existing in mammals is discussed.

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