Prolactin antagonizes the corticoid-promoted development of adult-type epidermis in cultured larval bullfrog skin.

EDTA-treated larval bullfrog skin, in which apical and skein cells had been removed and only basal cells remained, was cultured in one of four media. These contained either aldosterone (Aldo) or a mixture of Aldo, hydrocortisone (HC) and corticosterone (C), each either supplemented with prolactin (PRL) or lacking PRL. Skin cultured with Aldo alone or with the corticoid mixture (Aldo + HC + C) developed an adult-type epidermis: (i) both types of skin reacted to human blood group antigen A, a marker for the adult-type epidermis of bullfrog skin; (ii) amiloride decreased the short-circuit current Isc in these skin preparations, but acetylcholine (ACh) had no effect on the Isc. It seemed to make little difference to the results whether the skin was cultured with Aldo or with the corticoid mixture. PRL antagonized the action of Aldo and induced the development of a larval-type epidermis in both skin preparations: (i) the skin preparations did not react to human blood group antigen A; (ii) acetylcholine and amiloride each stimulated Isc in these preparations. Since ACh and amiloride each stimulated the Isc in skin with apical cells, ACh/amiloride-stimulated channels may be located on these cells.

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The Cla(Caldwell) Antigen : a New and Rare Human Blood Group Antigen related to the MNSs System

Nature ◽

10.1038/200689a0 ◽

1963 ◽

Vol 200 (4907) ◽

pp. 689-690 ◽

Cited By ~ 9

Author(s):

JOHN WALLACE ◽

MARIAN M. IZATT

Keyword(s):

Blood Group ◽

Human Blood ◽

Blood Group Antigen ◽

Human Blood Group ◽

Antigen A

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Prolactin enables normal development of ACh-stimulated current in cultured larval bullfrog skin

AJP Cell Physiology ◽

10.1152/ajpcell.1996.271.4.c1059 ◽

1996 ◽

Vol 271 (4) ◽

pp. C1059-C1063 ◽

Cited By ~ 5

Author(s):

M. Takada ◽

H. Yai ◽

K. Takayama-Arita ◽

S. Komazaki

Keyword(s):

Normal Development ◽

Short Circuit ◽

Short Circuit Current ◽

Starting Material ◽

Basal Cells ◽

Adult Type ◽

Larval Type ◽

Starting Point ◽

Stimulation Of

The response to acetylcholine (ACh) can be used as a marker for larval-type bullfrog skin because apically applied ACh induces an increase in short-circuit current (SCC) in larval-type but not adult-type skin. EDTA-treated larval skin, which contains only basal cells and does not respond to ACh, was used as the starting material for our culture. ACh, carbamylcholine, and choline stimulated SCC in skin that had been cultured with aldosterone (5 x 10(-7) M) supplemented with prolactin (PRL; 2 micrograms/ml). Atropine and d-tubocurarine each inhibited the ACh-induced stimulation of SCC in skin so cultured. Eserine, an inhibitor of acetylcholinesterase, also inhibited the ACh response. Amiloride stimulated SCC itself, but it reduced the ACh response. All of these results are quite similar to those seen in intact larval skin, suggesting that a larval-skin had differentiated from the basal cells used as the starting point for our culture. This is the first physiological report that PRL induces differentiation in vitro into a true larval-type bullfrog skin.

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Prolactin inhibits corticoid-induced differentiation of active Na+ transport across cultured frog tadpole skin

AJP Cell Physiology ◽

10.1152/ajpcell.1995.269.5.c1326 ◽

1995 ◽

Vol 269 (5) ◽

pp. C1326-C1331 ◽

Cited By ~ 6

Author(s):

M. Takada ◽

H. Yai ◽

K. Takayama-Arita

Keyword(s):

Acetylcholine Receptor ◽

Inhibitory Action ◽

Blood Group Antigen ◽

The Other ◽

Basal Cells ◽

Na Transport ◽

Adult Type ◽

Frog Tadpole ◽

Antigen A ◽

Tadpole Skin

Active Na+ transport differentiates in larval bullfrog skin cultured with corticoids. After 2 wk in culture, the epidermis became positive against human blood group antigen A, the marker for the adult-type cells of the epidermis, but was negative to the antibody against the acetylcholine receptor, the marker for the larval-type epidermis. Amiloride (10(-5) M) did not inhibit the differentiation of active Na+ transport. On the other hand, in skin cultured with prolactin (2 micrograms/ml), the epidermis remained negative against antigen A and positive against acetylcholine receptor, and the differentiation of active Na+ transport was inhibited. Thyroid hormone did not antagonize the inhibitory action of prolactin on this transport differentiation. Prolactin affected the basal cells of the larval epidermis and inhibited development of corticoid-induced adult features in the epidermis.

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