scholarly journals Characterization of Factors that Suppress Linear DNA Replication in SV40 in vitro Replication System.

1993 ◽  
Vol 18 (1) ◽  
pp. 19-32 ◽  
Author(s):  
Shinji Katsura ◽  
Toshihiko Eki ◽  
Hajime Nishimura ◽  
Yasufumi Murakami
Keyword(s):  
Dna Replication ◽  
Replication System ◽  
Linear Dna ◽  
In Vitro Replication

scholarly journals Characterization of an improved in vitro DNA replication system for Escherichia coli plasmids

1979 ◽  
Vol 6 (10) ◽  
pp. 3289-3304 ◽  
Author(s):  
Susan E. Conrad ◽  
Judith L. Campbell
Keyword(s):  
Escherichia Coli ◽  
Dna Replication ◽  
Replication System

Fidelity of DNA synthesis in a mammalian in vitro replication system

1988 ◽  
Vol 8 (8) ◽  
pp. 3267-3271
Author(s):  
J Hauser ◽  
A S Levine ◽  
K Dixon
Keyword(s):  
Dna Replication ◽  
Shuttle Vector ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
Base Substitution ◽  
Replication System ◽  
In Vitro Replication ◽  
Mutation Assay

We have used the simian virus 40 (SV40)-based shuttle vector pZ189 in a forward-mutation assay to determine the fidelity of DNA replication in the in vitro DNA replication system developed by J.J. Li and T.J. Kelly (Proc. Natl. Acad. Sci. USA 81:6973-6977, 1984). We find that very few base substitution errors (approximately 1/180,000 bases incorporated) are made during in vitro replication of the pZ189 vector in a system derived from CV-1 monkey cells. This replication is completely dependent on added SV40 T antigen and presumably reflects synthesis that is initiated at the SV40 replication origin. The observed level of fidelity is far greater than that reported for in vitro replication of DNA by conventionally purified eucaryotic DNA polymerases alpha and beta. Thus, there must be additional cellular factors in the crude in vitro system that serve to enhance the fidelity of DNA replication.

scholarly journals Fidelity of DNA synthesis in a mammalian in vitro replication system.

1988 ◽  
Vol 8 (8) ◽  
pp. 3267-3271 ◽  
Author(s):  
J Hauser ◽  
A S Levine ◽  
K Dixon
Keyword(s):  
Dna Replication ◽  
Shuttle Vector ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
Base Substitution ◽  
Replication System ◽  
In Vitro Replication ◽  
Mutation Assay

We have used the simian virus 40 (SV40)-based shuttle vector pZ189 in a forward-mutation assay to determine the fidelity of DNA replication in the in vitro DNA replication system developed by J.J. Li and T.J. Kelly (Proc. Natl. Acad. Sci. USA 81:6973-6977, 1984). We find that very few base substitution errors (approximately 1/180,000 bases incorporated) are made during in vitro replication of the pZ189 vector in a system derived from CV-1 monkey cells. This replication is completely dependent on added SV40 T antigen and presumably reflects synthesis that is initiated at the SV40 replication origin. The observed level of fidelity is far greater than that reported for in vitro replication of DNA by conventionally purified eucaryotic DNA polymerases alpha and beta. Thus, there must be additional cellular factors in the crude in vitro system that serve to enhance the fidelity of DNA replication.

scholarly journals Development of an in vitro bacteriophage N4 DNA replication system.

1986 ◽  
Vol 261 (23) ◽  
pp. 10506-10510
Author(s):  
J K Rist ◽  
M Pearle ◽  
A Sugino ◽  
L B Rothman-Denes
Keyword(s):  
Dna Replication ◽  
Replication System ◽  
Bacteriophage N4

Phenotypic and functional characterization of T-cells and in vitro replication of FMDV serotypes in bovine lymphocytes

Vaccine ◽  
2009 ◽  
Vol 27 (48) ◽  
pp. 6656-6661 ◽  
Author(s):  
Gurudutt Joshi ◽  
Ravindra Sharma ◽  
Naresh Kumar Kakker
Keyword(s):  
T Cells ◽  
Functional Characterization ◽  
In Vitro Replication ◽  
Fmdv Serotypes ◽  
Bovine Lymphocytes
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