Isolation and Characterization of an in Vitro DNA Replication System from Maize Mitochondria

1995 ◽  
Vol 208 (1) ◽  
pp. 287-294 ◽  
Author(s):  
H. Daniell ◽  
D. Zheng ◽  
B.L. Nielsen
Keyword(s):  
Dna Replication ◽  
Replication System ◽  
Isolation And Characterization

scholarly journals Characterization of an improved in vitro DNA replication system for Escherichia coli plasmids

1979 ◽  
Vol 6 (10) ◽  
pp. 3289-3304 ◽  
Author(s):  
Susan E. Conrad ◽  
Judith L. Campbell
Keyword(s):  
Escherichia Coli ◽  
Dna Replication ◽  
Replication System

scholarly journals Characterization of Factors that Suppress Linear DNA Replication in SV40 in vitro Replication System.

1993 ◽  
Vol 18 (1) ◽  
pp. 19-32 ◽  
Author(s):  
Shinji Katsura ◽  
Toshihiko Eki ◽  
Hajime Nishimura ◽  
Yasufumi Murakami
Keyword(s):  
Dna Replication ◽  
Replication System ◽  
Linear Dna ◽  
In Vitro Replication

PENGEMBANGAN TEKNOLOGI SELEKSI IN VITRO BERULANG (REPEAT CYCLING–IN VITRO SELECTION) TOLERAN STRES KEKERINGAN UNTUK PERBAIKAN MUTU GENETIK DAN PRODUKSI TANAMAN KEDELAI BERMIKORIZA

2017 ◽  
Vol 1 (1) ◽  
pp. 74-84
Author(s):  
Ahmad Riduan ◽  
Rainiyati Rainiyati ◽  
Yulia Alia
Keyword(s):  
Arbuscular Mycorrhizae ◽  
In Vitro Selection ◽  
Soil Samples ◽  
Single Spore ◽  
Isolation And Characterization ◽  
Single Spore Culture ◽  
Spore Culture ◽  
Glomus Sp

Every plant rhizospheres in any ecosystem there are various living microorganisms including Arbuscular Mycorrhizae Fungi (AMF).  An isolation and characterization is required to investigate the species or type of the AMF. This research was aimed at studying the isolation and characterization of AMF sporulation in soybean rhizospheres in Jambi Province. The results of evaluation on soil samples before trapping showed that there are spores from three genus of AMF twelve types Glomus , two types Acaulospora and one type of Enthrophospora.  Following single spore culture in soybean rhizosphere, 5 spore types were obtained:  Glomus sp-1, Glomus sp-4, Glomus sp-7, Glomus sp-8 Glomus sp-10.

scholarly journals Development of an in vitro bacteriophage N4 DNA replication system.

1986 ◽  
Vol 261 (23) ◽  
pp. 10506-10510
Author(s):  
J K Rist ◽  
M Pearle ◽  
A Sugino ◽  
L B Rothman-Denes
Keyword(s):  
Dna Replication ◽  
Replication System ◽  
Bacteriophage N4

Arginine synthesis by hepatomas in vitro. II. Isolation and characterization of Morris hepatoma variants unable to convert ornithine to arginine, and modulation of urea-cycle enzymes by dexamethasone and cyclic-AMP

1984 ◽  
Vol 68 (1) ◽  
pp. 305-319
Author(s):  
S.J. Goss
Keyword(s):  
Cyclic Amp ◽  
Liver Cells ◽  
Isolation And Characterization ◽  
Rat Liver Cells ◽  
Morris Hepatoma ◽  
Ornithine Transcarbamoylase ◽  
Cellular Incorporation

‘77orn’, a derivative of the Morris rat hepatoma 7777, stably expresses high levels of ornithine transcarbamoylase (OTC) and carbamoylphosphate synthetase I (CPS-I), and is able to grow indefinitely in ornithine-medium (medium with ornithine in place of arginine). Variants that have lost this ability are isolated from 77orn by a ‘suicide’ selective technique dependent on the cellular incorporation of [3H]ornithine. These variants, which have reduced levels of CPS-I, or of both CPS-I and OTC, are shown to have developed multiple hormonal requirements; their enzyme deficiencies can be reversed by use of an appropriately supplemented medium. In particular, CPS-I is inducible by dexamethasone and dibutyryl-cyclic-AMP in combination. Cholera toxin can be used instead of cyclic-AMP, but then butyrate is additionally required if the induction is to be maintained in the long term. The use of these agents in excess can depress OTC. Several other hepatomas, and alos explanted foetal rat liver cells, have similar requirements for CPS-I expression. It is argued that multiple hormonal requirements for CPS-I production are normal in liver cells in vitro, and that hormone-independent hepatomas should be regarded as abnormal. The implications of this for the somatic cell genetic investigation of differentiation are briefly discussed.

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