scholarly journals Human Liver High Molecular Weight Zinc-Dependent Acid p-Nitrophenylphosphatase. Purification and Properties.

1997 ◽  
Vol 20 (12) ◽  
pp. 1235-1239 ◽  
Author(s):  
Antonella ANGIOLILLO ◽  
Fausto PANARA
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Human Liver ◽  
Purification And Properties

scholarly journals Deoxyribonucleic acid polymerases of Euglena gracilis. Purification and properties of two distinct deoxyribonucleic acid polymerases of high molecular weight

1975 ◽  
Vol 151 (2) ◽  
pp. 227-238 ◽  
Author(s):  
A G McLennan ◽  
H M Keir
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Euglena Gracilis ◽  
Deoxyribonucleic Acid ◽  
Deae Cellulose ◽  
Low Molecular Weight ◽  
Optimum Conditions ◽  
Electrophoretic Mobilities ◽  
Substrate Activation ◽  
Purification And Properties

Two DNA polymerases of high molecular weight, pol A (mol.wt. 190 000) and pol B (mol.wt. 240 ooo), have been purified 6300-fold and 1600-fold respectively from an extramitochondrial supernatant of a bleached strain of Euglena gracilis. They have very similar requirements when assayed with an ‘activated’-DNA primer-template [the optimum conditions of pH and ionic (K+ and Mn2+) composition being 7.2, 25 mM and 0.2 mM respectively]. 0.2 mM-Mn2+ was about 1.5-2-fold as effective as 2 mM-Mg2+, owing to substrate activation by deoxyribonucleoside 5′-triphosphates in the presence of Mn2+. Km values for the triphosphates in the absence of activation were about 10(-6)M with Mn2+ and 8 × 10(-6) M with Mg2+ for both enzymes. They were inhibited to the same extent by N-ethylmaleimide, novobiocin and o-phenanthroline, but differed in their chromatographic behaviour on DEAE-cellulose and in their electrophoretic mobilities on polyacrylamide gel. No evidence was found for the existence in these cells of a DNA polymerase of low molecular weight, but there were indications that a third enzyme of high molecular weight might exist.

scholarly journals Purification and Properties of a Chromobacterium Lipase with a High Molecular Weight

1974 ◽  
Vol 38 (5) ◽  
pp. 947-952 ◽  
Author(s):  
Mamoru SUGIURA ◽  
Masakazu ISOBE ◽  
Noriyuki MUROYA ◽  
Tsutomu YAMAGUCHI
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Purification And Properties

Specific loss of the high-molecular-weight form ofS-adenosyl-L-methionine synthetase in human liver cirrhosis

Hepatology ◽  
1988 ◽  
Vol 8 (6) ◽  
pp. 1530-1534 ◽  
Author(s):  
Carmen Cabrero ◽  
Antonio Martin Duce ◽  
Pablo Ortiz ◽  
Susana Alemany ◽  
Jose M. Mato
Keyword(s):  
Molecular Weight ◽  
Liver Cirrhosis ◽  
High Molecular Weight ◽  
Human Liver ◽  
Specific Loss ◽  
High Molecular Weight Form ◽  
Human Liver Cirrhosis ◽  
Molecular Weight Form

Purification and properties of a high-molecular-weight, alkaline exopolygalacturonase from a strain of Bacillus

2001 ◽  
Vol 29 (1) ◽  
pp. 70-75 ◽  
Author(s):  
Tohru Kobayashi ◽  
Norihiko Higaki ◽  
Atsushi Suzumatsu ◽  
Kazuhisa Sawada ◽  
Hiroshi Hagihara ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Purification And Properties
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