scholarly journals The inhibitory effect of methanol on forskolin-activated adenylate cyclase in rat erythrocyte membranes dependent on the state of the guanine nucleotide-binding stimulatory and regulatory protein.

1988 ◽  
Vol 11 (5) ◽  
pp. 377-380 ◽  
Author(s):  
Osamu TAKEDA ◽  
Kazuo YAMASAKI ◽  
Hiroshi KOHDA ◽  
Atsushi YAMASHITA ◽  
Tomonori KUROKAWA ◽  
...  
Keyword(s):  
Adenylate Cyclase ◽  
Regulatory Protein ◽  
Nucleotide Binding ◽  
Inhibitory Effect ◽  
The State ◽  
Erythrocyte Membranes ◽  
Guanine Nucleotide ◽  
Guanine Nucleotide Binding

scholarly journals Restoration of guanine nucleotide- and fluoride-stimulated activity to an adenylate cyclase-deficient cell line with affinity-purified guanine nucleotide regulatory protein

1980 ◽  
Vol 190 (2) ◽  
pp. 439-443 ◽  
Author(s):  
T B Nielsen ◽  
R W Downs ◽  
A M Spiegel
Keyword(s):  
Adenylate Cyclase ◽  
Affinity Chromatography ◽  
Cell Line ◽  
Mutant Cell ◽  
Regulatory Protein ◽  
Erythrocyte Membranes ◽  
Guanine Nucleotide ◽  
Guanine Nucleotide Binding Protein ◽  
Guanine Nucleotide Binding ◽  
Guanine Nucleotide Regulatory Protein

A guanine nucleotide-binding protein purified from turkey erythrocytes by affinity chromatography confers both F— and guanine nucleotide-stimulation of adenylate cyclase to membranes from CYC- cells, a mutant cell line deficient in these responses. Interaction of turkey erythrocyte membranes with beta-adrenergic agonists before affinity chromatography, which is essential for binding of the guanine nucleotide regulatory protein to the affinity matrix, was also required for recovery of F—stimulation restoring activity in the affinity eluate.

Sign in / Sign up

Export Citation Format

Share Document