scholarly journals Linezolid-induced Apoptosis through Mitochondrial Damage and Role of Superoxide Dismutase-1 in Human Monocytic Cell Line U937

2018 ◽  
Vol 138 (1) ◽  
pp. 73-81
Author(s):  
Satoshi Fujii ◽  
Sanae Muraoka ◽  
Atsushi Miyamoto ◽  
Koichi Sakurai
2005 ◽  
Vol 52 (3) ◽  
pp. 211-217 ◽  
Author(s):  
SABRINA LISI ◽  
MARGHERITA SISTO ◽  
ANGELA ACQUAFREDDA ◽  
ROSA SPINELLI ◽  
MARIA ANGELA SCHIAVONE ◽  
...  

1996 ◽  
Vol 15 (2-3) ◽  
pp. 115-122 ◽  
Author(s):  
Masahiro Ito ◽  
Tomomi Yamamoto ◽  
Masahiro Watanabe ◽  
Toshiaki Ihara ◽  
Hitoshi Kamiya ◽  
...  

10.1251/bpo92 ◽  
2004 ◽  
Vol 6 (1) ◽  
pp. 220-225 ◽  
Author(s):  
Neora Pick ◽  
Scott Cameron ◽  
Dorit Arad ◽  
Yossef Av-Gay

2019 ◽  
Author(s):  
Ana Neves-Costa ◽  
Dora Pedroso ◽  
Luis F Moita

Abstract This protocol details the experimental procedure for performing the comet assay, a very sensitive DNA break assay based on single cell gel electrophoresis.The analysis of DNA strand breaks, both single- and double-strand breaks (SSBs and DSBs, respectively), was performed in immune responsive cells. The cell line used was the human monocytic cell line THP-1, an adherent cell type with many known applications in in vitro studies of innate immunity. The comet assay is a robust procedure that allows the accurate and reproducible quantification of DNA damage. Here we describe not only the comet assay step-by-step protocol, but also some important aspects related to troubleshooting.


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