Role of Interferon-γ against Invasion byToxoplasma gondiiin a Human Monocytic Cell Line (THP1): Involvement of the Parasite's Secretory Phospholipase A2

1996 ◽  
Vol 169 (2) ◽  
pp. 218-225 ◽  
Author(s):  
Jorge E.Gómez Marı́n ◽  
Annie Bonhomme ◽  
Moncef Guenounou ◽  
Jean Michel Pinon
Keyword(s):  
Phospholipase A2 ◽  
Cell Line ◽  
Interferon Γ ◽  
Secretory Phospholipase A2 ◽  
Monocytic Cell ◽  
Monocytic Cell Line ◽  
Secretory Phospholipase ◽  
Human Monocytic Cell Line ◽  
Human Monocytic Cell

scholarly journals Induction of synthesis of components of the hydrogen peroxide-generating oxidase during activation of the human monocytic cell line U937 by interferon-γ

1987 ◽  
Vol 248 (1) ◽  
pp. 281-283 ◽  
Author(s):  
P W Andrew ◽  
A K Robertson ◽  
D B Lowrie ◽  
A R Cross ◽  
O T G Jones
Keyword(s):  
Hydrogen Peroxide ◽  
Cell Line ◽  
Cytochrome B ◽  
Time Course ◽  
Interferon Γ ◽  
Monocytic Cell ◽  
Monocytic Cell Line ◽  
Human Monocytic Cell Line ◽  
Human Monocytic Cell ◽  
Activity Induction

Increased amounts of cytochrome b-245 and a 45 kDa polypeptide component of the hydrogen peroxide-generating oxidase were detected in the human monocytic cell line U937 after incubation in interferon-gamma. The time course of increase in cytochrome b-245 paralleled the induction of oxidase activity. Induction of the 45 kDa component had a different time course, having a greater lag before increased amounts were seen.

scholarly journals Screening of compounds toxicity against human Monocytic cell line-THP-1 by flow cytometry

10.1251/bpo92 ◽  
2004 ◽  
Vol 6 (1) ◽  
pp. 220-225 ◽  
Author(s):  
Neora Pick ◽  
Scott Cameron ◽  
Dorit Arad ◽  
Yossef Av-Gay
Keyword(s):  
Flow Cytometry ◽  
Cell Line ◽  
Monocytic Cell ◽  
Monocytic Cell Line ◽  
Human Monocytic Cell Line ◽  
Human Monocytic Cell

scholarly journals Alkaline Comet Assay using the monocytic cell line THP-1

2019 ◽  
Author(s):  
Ana Neves-Costa ◽  
Dora Pedroso ◽  
Luis F Moita
Keyword(s):  
Comet Assay ◽  
Cell Line ◽  
Adherent Cell ◽  
Monocytic Cell ◽  
Monocytic Cell Line ◽  
Strand Breaks ◽  
Human Monocytic Cell Line ◽  
Human Monocytic Cell ◽  
Dna Strand

Abstract This protocol details the experimental procedure for performing the comet assay, a very sensitive DNA break assay based on single cell gel electrophoresis.The analysis of DNA strand breaks, both single- and double-strand breaks (SSBs and DSBs, respectively), was performed in immune responsive cells. The cell line used was the human monocytic cell line THP-1, an adherent cell type with many known applications in in vitro studies of innate immunity. The comet assay is a robust procedure that allows the accurate and reproducible quantification of DNA damage. Here we describe not only the comet assay step-by-step protocol, but also some important aspects related to troubleshooting.

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