Continuous Stereoselective Reduction Catalyzed by Thermophilic Alcohol Dehydrogenase in a Gas Phase Bioreactor

2011 ◽  
Vol 44 (12) ◽  
pp. 995-998 ◽  
Author(s):  
Kazuhito Nagayama ◽  
Antje C. Spieß ◽  
Jochen Büchs
Keyword(s):  
Alcohol Dehydrogenase ◽  
Gas Phase ◽  
Stereoselective Reduction

Preparation and Pyrolysis of Stereoisomeric Bromobicyclo[n.1.0]alkanes ( n = 6, 8 , 10)

1987 ◽  
Vol 42 (4) ◽  
pp. 489-494 ◽  
Author(s):  
Eckehard V. Dehmlow ◽  
Roland Kramer
Keyword(s):  
Thermal Stability ◽  
Gas Phase ◽  
Condensed Phase ◽  
Secondary Products ◽  
Gas Phase Reactions ◽  
Stereoselective Reduction ◽  
Derivatives Of ◽  
Thermal Stability Of

Abstract The title compounds la-3c were prepared by stereoselective reduction of the respective dibromides. Pyrolysis gave allylic bromides (8, 9, 11) as primary and dienes (10, 12) as secondary products. Product ratios were independent of the stereochemistry of the starting materials. No differences of the rearrangement rates of the stereoisomers were observed in gas phase reactions of the derivatives of bicyclo[6.1.0]- and bicyclo[8.1.0]alkanes. With the larger bicyclo[10.1.0] derivatives, however, distinct differences in the thermal stability of cis-trans-isomers4c/5c or 2c/3c were found in condensed phase.

Enzyme technology and gas phase catalysis: Alcohol dehydrogenase example

1986 ◽  
Vol 8 (11) ◽  
pp. 783-784 ◽  
Author(s):  
S. Pulvin ◽  
M. D. Legoy ◽  
R. Lortie ◽  
M. Pensa ◽  
D. Thomas
Keyword(s):  
Alcohol Dehydrogenase ◽  
Gas Phase ◽  
Enzyme Technology

Acetone Bio-Sniffer (Gas-Phase Biosensor) for Monitoring of Human Volatile Using Enzymatic Reaction of Secondary Alcohol Dehydrogenase

2021 ◽  
Vol 6 (1) ◽  
pp. 45
Author(s):  
Takahiro Arakawa ◽  
Ming Ye ◽  
Kenta Iitani ◽  
Koji Toma ◽  
Kohji Mitsubayashi
Keyword(s):  
Alcohol Dehydrogenase ◽  
Gas Phase ◽  
Secondary Alcohol ◽  
Enzymatic Reaction ◽  
Flow Cell ◽  
Exhaled Breath ◽  
Acetone Vapor ◽  
Acetone Concentration ◽  
Secondary Alcohol Dehydrogenase ◽  
Breath Acetone

We developed a highly sensitive acetone bio-sniffer (gas-phase biosensor) based on an enzyme reductive reaction to monitor breath acetone concentration. The acetone bio-sniffer device was constructed by attaching a flow-cell with nicotinamide adenine dinucleotide (NADH)-dependent secondary alcohol dehydrogenase (S-ADH) immobilized membrane onto a fiber-optic NADH measurement system. This system utilizes an ultraviolet light emitting diode as an excitation light source. Acetone vapor was measured as the fluorescence of NADH consumption by the enzymatic reaction of S-ADH. A phosphate buffer that contained oxidized NADH was circulated into the flow-cell to rinse the products and the excessive substrates from the optode; thus, the bio-sniffer enables the real-time monitoring of acetone vapor concentration. A photomultiplier tube detects the change in the fluorescence emitted from NADH. The relationship between the fluorescence intensity and acetone concentration was identified to be from 20 ppb to 5300 ppb. This encompasses the range of concentration of acetone vapor found in the breath of healthy people and of those suffering from disorders of carbohydrate metabolism. Then, the acetone bio-sniffer was used to monitor the exhaled breath acetone concentration change before and after a meal. When the sensing region was exposed to exhaled breath, the fluorescence intensity decreased and reached saturation immediately. Then, it returned to the initial state upon cessation of the exhaled breath flow. We anticipate its future use as a non-invasive analytical tool for the assessment of lipid metabolism in exercise, fasting and diabetes mellitus.

Reactivity of Immobilized Alcohol Dehydrogenase Containing Polyvinyl Alcohol in a Gas Phase Bioreactor

2013 ◽  
Vol 39 (4) ◽  
pp. 368-371 ◽  
Author(s):  
Kazuhito Nagayama ◽  
Kenichiro Kurio ◽  
Kyohei Morita
Keyword(s):  
Alcohol Dehydrogenase ◽  
Polyvinyl Alcohol ◽  
Gas Phase
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