Dose-dependent and time-dependent changes in the choroid plexus of the irradiated rat brain

Assessment of Cyto/Genotoxicity of Irinotecan in V79 Cells Using the Comet, Micronucleus, and Chromosome Aberration AssayIrinotecan is a topoisomerase I interactive agent, widely used in the treatment of metastatic colorectal cancer. The genotoxic effects of the maximum single dose (18 μg mL-1), recommended monotherapy dose (9 μg mL-1), and recommended combined therapy dose (4.5 μg mL-1) of irinotecan were studied on V79 cells using the comet assay, chromosome aberration assay, and micronucleus test. The cells were treated with irinotecan for 2 h or 24 h. The statistical significance of the results was determined using the one-way ANOVA test and a nonparametric Mann Whitney U test. The comet assay did not show dose-dependent or time-dependent effects. The chromosome aberration analysis showed large DNA rearrangements, i.e., chromosome exchanges. Although the exposed cultures showed a significant increase in micronucleated cells in respect to control, no dose-dependent relation was established among the treated cultures. Time-dependent effect was also not observed.

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Erratum to: Dose-dependent sigma-1 receptor occupancy by donepezil in rat brain can be assessed with 11C-SA4503 and microPET

Psychopharmacology ◽

10.1007/s00213-014-3559-5 ◽

2014 ◽

Vol 231 (20) ◽

pp. 4085-4085

Author(s):

Nisha K. Ramakrishnan ◽

Anniek K. D. Visser ◽

Marianne Schepers ◽

Gert Luurtsema ◽

Csaba J. Nyakas ◽

...

Keyword(s):

Rat Brain ◽

Receptor Occupancy ◽

Sigma 1 Receptor ◽

Sigma 1 ◽

Dose Dependent

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Time-dependent cadmium-neurotoxicity in rat brain synaptosomal plasma membranes

Comparative Biochemistry and Physiology Part C Comparative Pharmacology ◽

10.1016/0742-8413(91)90167-r ◽

1991 ◽

Vol 100 (1-2) ◽

pp. 271-275 ◽

Cited By ~ 10

Author(s):

C.D. Fasitsas ◽

S.E. Theocharis ◽

D. Zoulas ◽

S. Chrissimou ◽

G. Deliconstantinos

Keyword(s):

Rat Brain ◽

Plasma Membranes ◽

Time Dependent ◽

Synaptosomal Plasma Membranes

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Synthesis of transthyretin (pre-albumin) mRNA in choroid plexus epithelial cells, localized by in situ hybridization in rat brain.

Journal of Histochemistry & Cytochemistry ◽

10.1177/34.7.3458812 ◽

1986 ◽

Vol 34 (7) ◽

pp. 949-952 ◽

Cited By ~ 65

Author(s):

A J Stauder ◽

P W Dickson ◽

A R Aldred ◽

G Schreiber ◽

F A Mendelsohn ◽

...

Keyword(s):

In Situ Hybridization ◽

Epithelial Cells ◽

Rat Brain ◽

Choroid Plexus ◽

Cellular Localization ◽

Lateral Ventricles ◽

The Brain ◽

Albumin Mrna ◽

Choroid Plexus Epithelial

The sites of synthesis of transthyretin in the brain were investigated using in situ hybridization with [35S]-labeled recombinant cDNA probes specific for transthyretin mRNA. Autoradiography of hybridized coronal sections of rat brain revealed specific cellular localization of transthyretin mRNA in choroid plexus epithelial cells of the lateral, third, and fourth ventricles. Transferrin mRNA was also investigated and, in contrast to transthyretin mRNA, was localized mainly in the lateral ventricles. Our results indicate that substantial synthesis of transthyretin and transferrin mRNA may occur in the choroid plexus.

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