scholarly journals Effects of Trichostatin A on In Vitro Development and DNA Methylation Level of the Satellite I Region of Swamp Buffalo (Bubalus bubalis) Cloned Embryos

2014 ◽  
Vol 60 (5) ◽  
pp. 336-341 ◽  
Author(s):  
Kanokwan SRIRATTANA ◽  
Mariena KETUDAT-CAIRNS ◽  
Takashi NAGAI ◽  
Masahiro KANEDA ◽  
Rangsun PARNPAI
Keyword(s):  
Dna Methylation ◽  
Methylation Level ◽  
Trichostatin A ◽  
Bubalus Bubalis ◽  
In Vitro Development ◽  
Swamp Buffalo ◽  
Vitro Development

scholarly journals Downregulation of DNA Methyltransferase 1 in Zona-Free Cloned Buffalo (Bubalus bubalis) Embryos by Small Interefering RNA Improves In Vitro Development But Does Not Alter DNA Methylation Level

2015 ◽  
Vol 17 (2) ◽  
pp. 89-94 ◽  
Author(s):  
Naresh L. Selokar ◽  
Monika Saini ◽  
Himanshu Agrawal ◽  
Prabhat Palta ◽  
Manmohan S. Chauhan ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Methylation Level ◽  
Dna Methyltransferase ◽  
Bubalus Bubalis ◽  
Dna Methyltransferase 1 ◽  
In Vitro Development ◽  
Vitro Development ◽  
Methyltransferase 1

scholarly journals α-Tocopherol and l-ascorbic acid increase the in vitro development of IVM/IVF swamp buffalo (Bubalus bubalis) embryos

animal ◽  
2008 ◽  
Vol 2 (10) ◽  
pp. 1486-1490 ◽  
Author(s):  
K. Saikhun ◽  
T. Faisaikarm ◽  
Z. Ming ◽  
K.H. Lu ◽  
Y. Kitiyanant
Keyword(s):  
Ascorbic Acid ◽  
Bubalus Bubalis ◽  
In Vitro Development ◽  
Swamp Buffalo ◽  
Vitro Development

Treatment of donor cells with trichostatin A improves in vitro development and reprogramming of buffalo (Bubalus bubalis) nucleus transfer embryos

2013 ◽  
Vol 80 (8) ◽  
pp. 878-886 ◽  
Author(s):  
Chan Luo ◽  
Fenghua Lu ◽  
Xiaoli Wang ◽  
Zhiqiang Wang ◽  
Xiangping Li ◽  
...  
Keyword(s):  
Trichostatin A ◽  
Bubalus Bubalis ◽  
In Vitro Development ◽  
Donor Cells ◽  
Nucleus Transfer ◽  
Vitro Development

Pregnancy and calving rates following transfer of in-vitro-produced river and F1 (river × swamp) buffalo (Bubalus bubalis) embryos in recipients on natural oestrus or synchronised for ovulation

2007 ◽  
Vol 19 (5) ◽  
pp. 670 ◽  
Author(s):  
Xianwei Liang ◽  
Xiufang Zhang ◽  
Bingzhuang Yang ◽  
Mingtang Cheng ◽  
Fenxiang Huang ◽  
...  
Keyword(s):  
Bubalus Bubalis ◽  
Sperm Cells ◽  
In Vitro Production ◽  
In Vitro Development ◽  
Antral Follicles ◽  
Swamp Buffaloes ◽  
Swamp Buffalo ◽  
Vitro Development ◽  
Vitro Production

The main objective of this study was to compare pregnancy and calving rates following transfer of in-vitro-produced fresh river and F1 (river × swamp) buffalo embryos in recipients synchronised by Ovsynch protocol or following natural oestrus. River embryos were produced from cumulus–oocyte complexes (COCs) derived by ovum pick up (OPU) on 40 Murrah and Nili-Ravi donor buffaloes over a twice-weekly collection schedule for 120 single OPUs. F1 embryos were produced by fertilisation of swamp COCs recovered from abattoir ovaries coincubated with river sperm cells. Both groups of embryos were produced following the same protocol for in vitro production. With regard to the OPU source of COCs, 923 antral follicles were punctured and 647 COCs were recovered (70%). From 462 selected COCs for IVM, 257 (55.6%) cleaved zygotes were recorded leading to 93 blastocysts (20.1%). In total, 590 swamp COCs were aspirated from abattoir ovaries and 476 were selected for IVM leading to 270 (56.7%) cleaved zygotes and resulting in 137 blastocysts (28.8%). River and F1 embryos were transferred between Day 6 to 7 of in vitro development, corresponding to blastocyst–expanding blastocyst, into F1 recipients synchronised by Ovsynch and swamp buffaloes following natural oestrus, respectively, each of them receiving two embryos. According to palpation per rectum of the ovaries at the time of embryo transfer, 26 of the 47 (55.3%) F1 recipients synchronised by Ovsynch were considered suitable for transfer, resulting in seven pregnancies (26.9%) and four calvings (15.3%) owing to three abortions occurring between 2 and 3 months of pregnancy. In total, 29 swamp recipients following natural oestrus were judged suitable as recipients, resulting in 12 pregnancies (41.4%) and 10 calvings (34.5%) owing to two abortions at 2 and 3 months of gestation respectively. Pregnancy and calving rates following transfer of river and F1 embryos were similar. Likewise, weight at birth of calves derived from transfer of river and F1 embryos was not different: 30.5 ± 1.4 and 32.9 ± 2.4 respectively. Pregnancy and calving rates following AI in a group of river and swamp buffaloes considered for reference in this study were similar to recipients carrying in-vitro-produced embryos. Collectively, no apparent postnatal complications were recorded in resulting live calves.

Use of trichostatin A alters the expression of HDAC3 and KAT2 and improves in vitro development of bovine embryos cloned using less methylated mesenchymal stem cells

2018 ◽  
Vol 54 (2) ◽  
pp. 289-299 ◽  
Author(s):  
Carolina Gonzales da Silva ◽  
Carlos Frederico Martins ◽  
Heidi Christina Bessler ◽  
Álvaro Moraes da Fonseca Neto ◽  
Tereza Cristina Cardoso ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Trichostatin A ◽  
Bovine Embryos ◽  
In Vitro Development ◽  
Vitro Development

scholarly journals Comparison Between the Effects of Valproic Acid and Trichostatin A on in vitro Development of Sheep Somatic Cell Nuclear Transfer Embryos

2012 ◽  
Vol 11 (11) ◽  
pp. 1868-1872 ◽  
Author(s):  
Chuangfu Chen ◽  
Shengwei Hu ◽  
Wei Ni ◽  
Wujiafu Sai ◽  
Wureli Hazi ◽  
...  
Keyword(s):  
Valproic Acid ◽  
Somatic Cell ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Trichostatin A ◽  
In Vitro Development ◽  
Vitro Development

scholarly journals The HDAC Inhibitor LAQ824 Enhances Epigenetic Reprogramming and In Vitro Development of Porcine SCNT Embryos

2017 ◽  
Vol 41 (3) ◽  
pp. 1255-1266 ◽  
Author(s):  
Jun-Xue Jin ◽  
Sanghoon Lee ◽  
Anukul Taweechaipaisankul ◽  
Geon A. Kim ◽  
Byeong Chun Lee
Keyword(s):  
Dna Methylation ◽  
Formation Rate ◽  
Nuclear Reprogramming ◽  
In Vitro Development ◽  
Histone 3 ◽  
Expression Of Genes ◽  
Epigenetic Remodeling ◽  
Low Efficiency ◽  
Vitro Development

Background/Aims: Hypoacetylation caused by aberrant epigenetic nuclear reprogramming results in low efficiency of mammalian somatic cell nuclear transfer (SCNT). Many epigenetic remodeling drugs have been used in attempts to improve in vitro development of porcine SCNT embryos. In this study, we examined the effects of LAQ824, a structurally novel histone acetylase inhibitor, on the nuclear reprogramming and in vitro development of porcine SCNT embryos. Methods: LAQ824 treatment was supplemented during the culture of SCNT embryos. The reprogramming levels were measured by immunofluorescence and quantified by image J software. Relative expression levels of 18 genes were analyzed by quantitative real-time PCR. Results: 100 nM LAQ824 treatment of post-activation SCNT embryos for 24 h significantly improved the subsequent blastocyst formation rate. The LAQ824 treatment enhanced histone 3 lysine 9 (H3K9) levels, histone 4 lysine 12 (H4K12) levels, and reduced global DNA methylation levels as well as anti-5-methylcytosine (5-mC) at the pseudo-pronuclear and 2-cell stages. Furthermore, LAQ824 treatment positively regulated the mRNA expression of genes for histone acetylation (HAT1, HDAC1, 2, 3, and 6), DNA methylation (DNMT1, 3a and 3b), development (Pou5f1, Nanog, Sox2, and GLUT1) and apoptosis (Bax, Bcl2, Caspase 3 and Bak) in blastocysts. Conclusion: Optimum exposure (100 nM for 24 h) to LAQ824 post-activation improved the in vitro development of porcine SCNT embryos by enhancing levels of H3K9 and H4K12, reducing 5-mC, and regulating gene expression.

Roscovitine Treatment Improves Synchronization of Donor Cell Cycle in G0/G1 Stage and In Vitro Development of Handmade Cloned Buffalo (Bubalus bubalis) Embryos

2012 ◽  
Vol 14 (2) ◽  
pp. 146-154 ◽  
Author(s):  
Naresh L. Selokar ◽  
Monika Saini ◽  
Mushariffa Muzaffer ◽  
G. Krishnakanth ◽  
Ambika P. Saha ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Donor Cell ◽  
Bubalus Bubalis ◽  
In Vitro Development ◽  
Vitro Development

Effects of Trichostatin A on In Vitro Development of Porcine Parthenogenetic and Nuclear Transfer Embryos

2013 ◽  
Vol 37 (2) ◽  
pp. 57-64
Author(s):  
Yun-Fei Diao ◽  
◽  
Naruse Kenji ◽  
Rong-Xun Han ◽  
Tao- Lin ◽  
...  
Keyword(s):  
Nuclear Transfer ◽  
Trichostatin A ◽  
In Vitro Development ◽  
Vitro Development
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