scholarly journals Comparative studies of protein components in rat uterine fluid, serum, colostrum whey and intestinal fluid.

1977 ◽  
Vol 22 (4) ◽  
pp. 145-149
Author(s):  
Yoshihisa HASEGAWA ◽  
Shichiro SUGAWARA ◽  
Saburo TAKEUCHI
1961 ◽  
Vol 12 (2) ◽  
pp. 142-150 ◽  
Author(s):  
Henry J. Albers ◽  
Manuel Neves e Castro

Contraception ◽  
1993 ◽  
Vol 48 (5) ◽  
pp. 494-512 ◽  
Author(s):  
Peter F. Tauber ◽  
G. Manfred ◽  
U. Cramer ◽  
Lourens J.D. Zaneveld

1976 ◽  
Vol 22 (3) ◽  
pp. 109-114
Author(s):  
Yoshihisa HASEGAWA ◽  
Shichiro SUGAWARA ◽  
Saburo TAKEUCHI

Author(s):  
Wah Chiu ◽  
David Grano

The periodic structure external to the outer membrane of Spirillum serpens VHA has been isolated by similar procedures to those used by Buckmire and Murray (1). From SDS gel electrophoresis, we have found that the isolated fragments contain several protein components, and that the crystalline structure is composed of a glycoprotein component with a molecular weight of ∽ 140,000 daltons (2). Under an electron microscopic examination, we have visualized the hexagonally-packed glycoprotein subunits, as well as the bilayer profile of the outer membrane. In this paper, we will discuss some structural aspects of the crystalline glycoproteins, based on computer-reconstructed images of the external cell wall fragments.The specimens were prepared for electron microscopy in two ways: negatively stained with 1% PTA, and maintained in a frozen-hydrated state (3). The micrographs were taken with a JEM-100B electron microscope with a field emission gun. The minimum exposure technique was essential for imaging the frozen- hydrated specimens.


Author(s):  
Daniel Beniac ◽  
George Harauz

The structures of E. coli ribosomes have been extensively probed by electron microscopy of negatively stained and frozen hydrated preparations. Coupled with quantitative image analysis and three dimensional reconstruction, such approaches are worthwhile in defining size, shape, and quaternary organisation. The important question of how the nucleic acid and protein components are arranged with respect to each other remains difficult to answer, however. A microscopical technique that has been proposed to answer this query is electron spectroscopic imaging (ESI), in which scattered electrons with energy losses characteristic of inner shell ionisations are used to form specific elemental maps. Here, we report the use of image sorting and averaging techniques to determine the extent to which a phosphorus map of isolated ribosomal subunits can define the ribosomal RNA (rRNA) distribution within them.


1950 ◽  
Vol 16 (1) ◽  
pp. 91-103 ◽  
Author(s):  
John A. Layne ◽  
F.R. Schemm ◽  
W.W. Hurst

1972 ◽  
Vol 5 (1) ◽  
pp. 306-309
Author(s):  
Arthur J. O'Shea ◽  
Mervin D. Lynch ◽  
Thomas F. Harrington
Keyword(s):  

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