Improved production of ginsenosides in suspension cultures of ginseng by medium replenishment strategy

2008 ◽  
Vol 105 (3) ◽  
pp. 288-291 ◽  
Author(s):  
Cheol-Seung Jeong ◽  
Hosakatte Niranjana Murthy ◽  
Eun-Joo Hahn ◽  
Kee-Yoeup Paek
Keyword(s):  
Suspension Cultures ◽  
Replenishment Strategy ◽  
Medium Replenishment

Lignin release and photomixotrophism in suspension cultures of Picea abies

1992 ◽  
Vol 84 (3) ◽  
pp. 374-379 ◽  
Author(s):  
Liisa Kaarina Simola ◽  
Juha Lemmetyinen ◽  
Arja Santanen
Keyword(s):  
Picea Abies ◽  
Suspension Cultures

Characterisation of extracellular polysaccharides from suspension cultures of members of the Poaceae

2020 ◽  
Author(s):  
Ian Sims ◽  
K Middleton ◽  
AG Lane ◽  
AJ Cairns ◽  
A Bacic
Keyword(s):  
Cultured Cells ◽  
Oryza Sativa L ◽  
Suspension Cultures ◽  
Exchange Chromatography ◽  
Phleum Pratense ◽  
Arabinogalactan Protein ◽  
Extracellular Polysaccharides ◽  
Type Ii ◽  
Hordeum Vulgare L ◽  
Suspension Cultured Cells

Microscopic examination of suspension-cultured cells of Phleum pratense L., Panicum miliaceum L., Phalaris aquatica L. and Oryza sativa L. showed that they were comprised of numerous root primordia. Polysaccharides secreted by these suspension cultures contained glycosyl linkages consistent with the presence of high proportions of root mucilage-like polysaccharides. In contrast, suspension-cultured cells of Hordeum vulgare L. contained mostly undifferentiated cells more typical of plant cells in suspension culture. The polysaccharides secreted by H. vulgare cultures contained mostly linkages consistent with the presence of glucuronoarabinoxylan. The soluble polymers secreted by cell-suspension cultures of Phleum pratense contained 70% carbohydrate, 14% protein and 6% inorganic material. The extracellular polysaccharides were separated into four fractions by anion-exchange chromatography using a gradient of imidazole-HCl at pH 7.0. From glycosyl-linkage analyses, five polysaccharides were identified: an arabinosylated xyloglucan (comprising 20% of the total polysaccharide), a glucomannan (6%), a type-II arabinogalactan (an arabinogalactan-protein; 7%), an acidic xylan (3%), and a root-slime-like polysaccharide, which contained features of type-II arabinogalactans and glucuronomannans (65%).

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