scholarly journals Genetic characterization and fine mapping of S25, a hybrid male sterility gene, on rice chromosome 12

2017 ◽  
Vol 92 (4) ◽  
pp. 205-212 ◽  
Author(s):  
Takahiko Kubo ◽  
Atsushi Yoshimura ◽  
Nori Kurata
Plant Gene ◽  
2019 ◽  
Vol 19 ◽  
pp. 100186
Author(s):  
Sai Rekha Kadirimangalam ◽  
Rahman Hifzur ◽  
Saraswathi R ◽  
Kumar M ◽  
Raveendran M ◽  
...  

2020 ◽  
Vol 10 (4) ◽  
pp. 1309-1318
Author(s):  
Tzu-Kai Lin ◽  
Ya-Ping Lin ◽  
Shun-Fu Lin

Male sterility has been widely used in hybrid seed production in Brassica, but not in B. rapa ssp. chinensis, and genetic models of male sterility for this subspecies are unclear. We discovered a spontaneous mutant in B. rapa ssp. chinensis. A series of progeny tests indicated that male sterility in B. rapa ssp. chinensis follows a three-allele model with BrMsa, BrMsb, and BrMsc. The male sterility locus has been mapped to chromosome A07 in BC1 and F2 populations through genotyping by sequencing. Fine mapping in a total of 1,590 F2 plants narrowed the male sterility gene BrMs to a 400 kb region, with two SNP markers only 0.3 cM from the gene. Comparative gene mapping shows that the Ms gene in B. rapa ssp. pekinensis is different from the BrMs gene of B. rapa ssp. chinensis, despite that both genes are located on chromosome A07. Interestingly, the DNA sequence orthologous to a male sterile gene in Brassica napus, BnRf, is within 400 kb of the BrMs locus. The BnRf orthologs of B. rapa ssp. chinensis were sequenced, and one KASP marker (BrMs_indel) was developed for genotyping based on a 14 bp indel at intron 4. Cosegregation of male sterility and BrMs_indel genotypes in the F2 population indicated that BnRf from B. napus and BrMs from B. rapa are likely to be orthologs. The BrMs_indel marker developed in this study will be useful in marker-assisted selection for the male sterility trait.


2010 ◽  
Vol 28 (2) ◽  
pp. 181-187 ◽  
Author(s):  
M. G. Chu ◽  
S. C. Li ◽  
S. Q. Wang ◽  
A. P. Zheng ◽  
Q. M. Deng ◽  
...  

2011 ◽  
Vol 123 (2) ◽  
pp. 231-238 ◽  
Author(s):  
Xinmei Zhang ◽  
Jian Wu ◽  
Hui Zhang ◽  
Yuan Ma ◽  
Aiguang Guo ◽  
...  

2017 ◽  
Vol 131 (2) ◽  
pp. 449-460 ◽  
Author(s):  
Yike Han ◽  
Fengyue Zhao ◽  
Shang Gao ◽  
Xianyun Wang ◽  
Aimin Wei ◽  
...  

2007 ◽  
Vol 115 (1) ◽  
pp. 113-118 ◽  
Author(s):  
Zhen Huang ◽  
Yufeng Chen ◽  
Bin Yi ◽  
Lu Xiao ◽  
Chaozhi Ma ◽  
...  

Genetics ◽  
1993 ◽  
Vol 134 (1) ◽  
pp. 261-275 ◽  
Author(s):  
D E Perez ◽  
C I Wu ◽  
N A Johnson ◽  
M L Wu

Abstract In this study, we address the question of whether there exist major genes that cause complete male sterility in the interspecific hybrids of Drosophila and, if they do, how these genes may be characterized at the molecular level. Our approach is to introgress small segments of the X chromosome from Drosophila mauritiana (or Drosophila sechellia) into Drosophila simulans by repeated backcrosses for more than 20 generations. The introgressions are monitored by both visible mutations and a series of DNA markers. We compare the extent of introgressions that cause male sterility with those that do not. If a major sterility factor exists, there should be a sharp boundary between these two classes of introgressions and their breakpoints should demarcate such a gene. Furthermore, if male sterility is the only major fitness effect associated with the introgression, recombination analysis should yield a pattern predicted by the classical three-point cross. Both the genetic and molecular analyses suggest the presence of a major sterility factor from D. mauritiana, which we named Odysseus (Ods), in the cytological interval of 16D. We thus formalize three criteria for inferring the existence of a major gene within an introgression: (1) complete penetrance of sterility, (2) complementarity in recombination analysis, and (3) physical demarcation. Introgressions of Ods from D. sechellia do not cause sterility. Twenty-two introgressions in our collection have breakpoints in this interval of about 500 kb, making it possible to delineate Ods more precisely for molecular identification. The recombination analysis also reveals the complexity of the introgressed segments--even relatively short ones may contain a second male sterility factor and partial viability genes and may also interfere with crossovers. The spermatogenic defects associated with Ods and/or a second factor were characterized by phase-contrast microscopy.


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