The Study of Intestinal Alkaline Phosphatase: Radioautographic Observation of the Intestinal Absorption of L-phenylalanine

Fasted adult male rats were used to study the effect of dietary proteins on intestinal alkaline phosphatase. Groups of animals were offered one of several proteins; lactalbumin, egg albumin, zein, gelatin, wheat gluten, casein, and vitellin. Control animals had cellulose fed to them. The rats were sacrificed six hours after they were given the different diets. Alkaline phosphatase determinations with intestinal homogenates indicated that the two phosphoproteins, casein and vitellin, elevated levels of the enzyme significantly above fasting levels. Possible interpretations of these findings are discussed.

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THE ROLE OF ALKALINE PHOSPHATASE IN INTESTINAL ABSORPTION: II. THE EFFECTS OF VARIOUS CARBOHYDRATES ON LEVELS OF THE ENZYME IN INTESTINAL MUCOSA

Canadian Journal of Biochemistry and Physiology ◽

10.1139/y54-070 ◽

1954 ◽

Vol 32 (1) ◽

pp. 621-624 ◽

Cited By ~ 3

Author(s):

Jules Tuba ◽

Nestor Dickie

Keyword(s):

Alkaline Phosphatase ◽

Intestinal Absorption ◽

Intestinal Mucosa ◽

Adult Male ◽

Enzyme Level ◽

Male Rats ◽

Significant Elevation ◽

Intestinal Alkaline Phosphatase

Adult male rats were deprived of food for five days. Various groups of these animals were then offered cellulose or cellulose mixed with one of seven other carbohydrates. Six hours after food was placed before them, the animals were killed by decapitation, and a portion of the intestine from each rat was homogenized. Intestinal alkaline phosphatase estimations indicated (1) that the activity of the enzyme was increased significantly by the ingestion of glucose, galactose, fructose, and mannose, whereas (2) there was not a statistically significant elevation of the enzyme level in those animals which had been fed cellulose, arabinose, xylose, or sucrose. This would appear to suggest that alkaline phosphatase is involved in the intestinal absorption of some monosaccharides.

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THE ROLE OF ALKALINE PHOSPHATASE IN INTESTINAL ABSORPTION: IV. THE EFFECTS OF VARIOUS PROTEINS ON LEVELS OF THE ENZYME IN INTESTINAL MUCOSA

Canadian Journal of Biochemistry and Physiology ◽

10.1139/o55-013 ◽

1955 ◽

Vol 33 (1) ◽

pp. 89-92 ◽

Cited By ~ 4

Author(s):

Jules Tuba ◽

Nester Dickie

Keyword(s):

Alkaline Phosphatase ◽

Intestinal Absorption ◽

Intestinal Mucosa ◽

Adult Male ◽

Wheat Gluten ◽

Male Rats ◽

Dietary Proteins ◽

Intestinal Alkaline Phosphatase ◽

Egg Albumin

Fasted adult male rats were used to study the effect of dietary proteins on intestinal alkaline phosphatase. Groups of animals were offered one of several proteins; lactalbumin, egg albumin, zein, gelatin, wheat gluten, casein, and vitellin. Control animals had cellulose fed to them. The rats were sacrificed six hours after they were given the different diets. Alkaline phosphatase determinations with intestinal homogenates indicated that the two phosphoproteins, casein and vitellin, elevated levels of the enzyme significantly above fasting levels. Possible interpretations of these findings are discussed.

Download Full-text

THE ROLE OF ALKALINE PHOSPHATASE IN INTESTINAL ABSORPTION: II. THE EFFECTS OF VARIOUS CARBOHYDRATES ON LEVELS OF THE ENZYME IN INTESTINAL MUCOSA

Canadian Journal of Biochemistry and Physiology ◽

10.1139/o54-070 ◽

1954 ◽

Vol 32 (6) ◽

pp. 621-624 ◽

Cited By ~ 7

Author(s):

Jules Tuba ◽

Nestor Dickie

Keyword(s):

Alkaline Phosphatase ◽

Intestinal Absorption ◽

Intestinal Mucosa ◽

Adult Male ◽

Enzyme Level ◽

Male Rats ◽

Significant Elevation ◽

Intestinal Alkaline Phosphatase

Adult male rats were deprived of food for five days. Various groups of these animals were then offered cellulose or cellulose mixed with one of seven other carbohydrates. Six hours after food was placed before them, the animals were killed by decapitation, and a portion of the intestine from each rat was homogenized. Intestinal alkaline phosphatase estimations indicated (1) that the activity of the enzyme was increased significantly by the ingestion of glucose, galactose, fructose, and mannose, whereas (2) there was not a statistically significant elevation of the enzyme level in those animals which had been fed cellulose, arabinose, xylose, or sucrose. This would appear to suggest that alkaline phosphatase is involved in the intestinal absorption of some monosaccharides.

Download Full-text