Faculty Opinions recommendation of Duodenal brush border intestinal alkaline phosphatase activity affects bicarbonate secretion in rats.

Abstract Macromolecular alkaline phosphatase (EC 3.1.3.1) was found in the serum of a patient suffering from myasthenia gravis (adult type II) complicated with thymoma, and was shown by immunoelectrophoresis to be bound to immunoglobulins A and G (IgG). Placental alkaline phosphatase, complexed with either the patient's serum or IgG purified from the patient's serum, remained at the origin on electrophoresis, with significant loss of activity. Intestinal alkaline phosphatase, complexed with either the patient's serum or the patient's IgG, migrated to a position similar to that of the macromolecular alkaline phosphatase in the patient's serum on electrophoresis. About 50% of the placental alkaline phosphatase activity was inhibited with 0.1-0.2 g of the patient's IgG per liter, but 6.93 g of the IgG per liter was required for about 20% inhibition of the intestinal alkaline phosphatase activity. The complex of intestinal alkaline phosphatase with the patient's IgG was fairly heat stable. From these results, we concluded that the macromolecular alkaline phosphatase in the patient's serum consisted of intestinal alkaline phosphatase and IgG that was specific for placental alkaline phosphatase.

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Presence of γ-glutamyltransferase in the renal microvascular compartment

Canadian Journal of Biochemistry ◽

10.1139/o81-053 ◽

1981 ◽

Vol 59 (6) ◽

pp. 383-386 ◽

Cited By ~ 19

Author(s):

P. D. Dass ◽

R. P. Misra ◽

T. C. Welbourne

Keyword(s):

Alkaline Phosphatase ◽

Phosphatase Activity ◽

Density Gradient ◽

Alkaline Phosphatase Activity ◽

Brush Border ◽

Sucrose Density Gradient ◽

Isolated Glomeruli ◽

Crude Homogenate ◽

Brush Border Enzyme ◽

Enzyme Alkaline Phosphatase

The association between the brush border enzyme alkaline phosphatase and γ-glutamyltransferase was determined by sucrose density gradient analysis of crude kidney homogenates, isolated glomeruli, and isolated microvessels. As previously established there is an overlap of these enzyme activities in the crude homogenate corresponding to a density of 1.17 g∙cm−3. In contrast, isolated glomeruli sedimented with a peak of 1.25 g∙cm−3 and exhibited γ-glutamyltransferase activity but little alkaline phosphatase activity; homogenizing isolated glomeruli shifted the fragments to a density coincident with that observed for the crude homogenate γ-glutamyltransferase peak. A second population of capillaries, isolated microvessels, were homogenized and analyzed on the sucrose density gradient. These fragments sedimented over the same range as crude homogenate γ-glutamyltransferase peak but were devoid of alkaline phosphatase activity and yet exhibited remarkable γ-glutamyltransferase activity. The results indicate homogenization of renal cortex results in a heterogenous collection of particles from both tubular and microvascular locations exhibiting γ-glutamyltransferase activity which overlap with the brush border alkaline phosphatase containing membranes. However, isolation of microvessels and glomeruli prior to homogenization allows separation of γ-glutamyltransferase from alkaline phosphatase activity; between 10 and 20% of the total homogenate γ-glutamyltransferase activity is estimated to be associated with the microvascular compartment.

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In vitro stimulation of apparent intestinal alkaline phosphatase activity by thyrocalcitonin

Clinica Chimica Acta ◽

10.1016/0009-8981(74)90426-4 ◽

1974 ◽

Vol 57 (2) ◽

pp. 171-173 ◽

Cited By ~ 5

Author(s):

C. Lechi ◽

G. De Bastiani ◽

M. Zatti

Keyword(s):

Alkaline Phosphatase ◽

Phosphatase Activity ◽

Alkaline Phosphatase Activity ◽

Intestinal Alkaline Phosphatase ◽

Stimulation Of

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Tissue alkaline phosphatase activity and expression in an experimental infant swine model of cardiopulmonary bypass with deep hypothermic circulatory arrest

10.21203/rs.3.rs-21432/v1 ◽

2020 ◽

Author(s):

Ludmila Khailova ◽

Justin Robison ◽

James Jaggers ◽

Richard Ing ◽

Scott Lawson ◽

...

Keyword(s):

Alkaline Phosphatase ◽

Cardiac Surgery ◽

Cardiopulmonary Bypass ◽

Phosphatase Activity ◽

Alkaline Phosphatase Activity ◽

Circulatory Arrest ◽

Deep Hypothermic Circulatory Arrest ◽

Hypothermic Circulatory Arrest ◽

Swine Model ◽

Intestinal Alkaline Phosphatase

Abstract Background: Infant cardiac surgery with cardiopulmonary bypass results in decreased circulating alkaline phosphatase that is associated with poor post-operative outcomes. Bovine intestinal alkaline phosphatase infusion represents a novel therapy for post-cardiac surgery organ injury. However, the effects of cardiopulmonary bypass and bovine-intestinal alkaline phosphatase infusion on tissue-level alkaline phosphatase activity/expression are unknown.Methods: Infant pigs (n=20) underwent cardiopulmonary bypass with deep hypothermic circulatory arrest followed by four hours of intensive care. Seven control animals underwent mechanical ventilation only. Cardiopulmonary bypass/deep hypothermic circulatory arrest animals were given escalating doses of bovine intestinal alkaline phosphatase infusion (0-25U/kg/hr; n=5/dose). Kidney, liver, ileum, jejunum, colon, heart and lung were collected for measurement of tissue alkaline phosphatase activity and mRNA.Results: Tissue alkaline phosphatase activity varied significantly across organs with the highest levels found in the kidney and small intestine. Cardiopulmonary bypass with deep hypothermic circulatory arrest resulted in decreased kidney alkaline phosphatase activity and increased lung alkaline phosphatase activity, with no significant changes in the other organs. Alkaline phosphatase mRNA expression was increased in both the lung and the ileum. The highest dose of bovine intestinal alkaline phosphatase resulted in increased kidney and liver tissue alkaline phosphatase activity.Conclusions: Changes in alkaline phosphatase activity after cardiopulmonary bypass with deep hypothermic circulatory arrest and bovine intestinal alkaline phosphatase delivery are tissue specific. Kidneys, lung, and ileal alkaline phosphatase appear most affected by cardiopulmonary bypass with deep hypothermic circulatory arrest and further research is warranted to determine the mechanism and biologic importance of these changes.

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Non-thermal effects of 0.1 THz radiation on intestinal alkaline phosphatase activity and conformation

Chinese Physics B ◽

10.1088/1674-1056/ab50fd ◽

2019 ◽

Vol 28 (12) ◽

pp. 128702

Author(s):

Xin-Xin Zhang ◽

Ming-Xia He ◽

Yu Chen ◽

Cheng Li ◽

Jin-Wu Zhao ◽

...

Keyword(s):

Alkaline Phosphatase ◽

Phosphatase Activity ◽

Alkaline Phosphatase Activity ◽

Thermal Effects ◽

Thz Radiation ◽

Intestinal Alkaline Phosphatase

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Alkaline phosphatase activity does not mediate phosphate transport in the renal-cortical brush-border membrane

Biochemical Journal ◽

10.1042/bj1900473 ◽

1980 ◽

Vol 190 (2) ◽

pp. 473-476 ◽

Cited By ~ 41

Author(s):

H S Tenenhouse ◽

C R Scriver ◽

E J Vizel

Keyword(s):

Alkaline Phosphatase ◽

Phosphatase Activity ◽

Alkaline Phosphatase Activity ◽

Brush Border ◽

Brush Border Membrane ◽

Membrane Vesicles ◽

Phosphate Transport ◽

Mouse Kidney ◽

Brush Border Membrane Vesicles ◽

Low Phosphorus

We studied (1) the effect of primary modulators of phosphate transport, namely the hypophosphataemic mouse mutant (Hyp) and low-phosphorus diet, on alkaline phosphatase activity in mouse renal-cortex brush-border membrane vesicles and (2) the effect of several primary inhibitors of alkaline phosphatase on phosphate transport. Brush-border membrane vesicles from Hyp-mouse kidney had 50% loss of Na+-dependent phosphate transport, but only 18% decrease in alkaline phosphatase activity. The low-phosphorus diet effectively stimulated Na+/phosphate co-transport in brush-border membrane vesicles (+ 118%), but increased alkaline phosphatase activity only slightly (+13%). Levamisole (0.1 mM) and EDTA (1.0 mM) inhibited brush-border membrane-vesicle alkaline phosphatase activity of 82% and 93% respectively, but had no significant effect on Na+/phosphate co-transport. We conclude that alkaline phosphatase does not play a direct role in phosphate transport across the brush-border membrane of mouse kidney.

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