THE ROLE OF ALKALINE PHOSPHATASE IN INTESTINAL ABSORPTION: IV. THE EFFECTS OF VARIOUS PROTEINS ON LEVELS OF THE ENZYME IN INTESTINAL MUCOSA

1955 ◽  
Vol 33 (1) ◽  
pp. 89-92 ◽  
Author(s):  
Jules Tuba ◽  
Nester Dickie

Fasted adult male rats were used to study the effect of dietary proteins on intestinal alkaline phosphatase. Groups of animals were offered one of several proteins; lactalbumin, egg albumin, zein, gelatin, wheat gluten, casein, and vitellin. Control animals had cellulose fed to them. The rats were sacrificed six hours after they were given the different diets. Alkaline phosphatase determinations with intestinal homogenates indicated that the two phosphoproteins, casein and vitellin, elevated levels of the enzyme significantly above fasting levels. Possible interpretations of these findings are discussed.


1955 ◽  
Vol 33 (1) ◽  
pp. 89-92 ◽  
Author(s):  
Jules Tuba ◽  
Nester Dickie

Fasted adult male rats were used to study the effect of dietary proteins on intestinal alkaline phosphatase. Groups of animals were offered one of several proteins; lactalbumin, egg albumin, zein, gelatin, wheat gluten, casein, and vitellin. Control animals had cellulose fed to them. The rats were sacrificed six hours after they were given the different diets. Alkaline phosphatase determinations with intestinal homogenates indicated that the two phosphoproteins, casein and vitellin, elevated levels of the enzyme significantly above fasting levels. Possible interpretations of these findings are discussed.



1954 ◽  
Vol 32 (1) ◽  
pp. 621-624 ◽  
Author(s):  
Jules Tuba ◽  
Nestor Dickie

Adult male rats were deprived of food for five days. Various groups of these animals were then offered cellulose or cellulose mixed with one of seven other carbohydrates. Six hours after food was placed before them, the animals were killed by decapitation, and a portion of the intestine from each rat was homogenized. Intestinal alkaline phosphatase estimations indicated (1) that the activity of the enzyme was increased significantly by the ingestion of glucose, galactose, fructose, and mannose, whereas (2) there was not a statistically significant elevation of the enzyme level in those animals which had been fed cellulose, arabinose, xylose, or sucrose. This would appear to suggest that alkaline phosphatase is involved in the intestinal absorption of some monosaccharides.



1954 ◽  
Vol 32 (6) ◽  
pp. 621-624 ◽  
Author(s):  
Jules Tuba ◽  
Nestor Dickie

Adult male rats were deprived of food for five days. Various groups of these animals were then offered cellulose or cellulose mixed with one of seven other carbohydrates. Six hours after food was placed before them, the animals were killed by decapitation, and a portion of the intestine from each rat was homogenized. Intestinal alkaline phosphatase estimations indicated (1) that the activity of the enzyme was increased significantly by the ingestion of glucose, galactose, fructose, and mannose, whereas (2) there was not a statistically significant elevation of the enzyme level in those animals which had been fed cellulose, arabinose, xylose, or sucrose. This would appear to suggest that alkaline phosphatase is involved in the intestinal absorption of some monosaccharides.



1955 ◽  
Vol 33 (1) ◽  
pp. 83-88 ◽  
Author(s):  
Nester Dickie ◽  
Margaret I. Robinson ◽  
Jules Tuba

Fasted rats were used in a study of the effect of fatty acids on intestinal and serum phosphatase. Each acid was mixed with a palatable carrier, and then offered to the animals. Enzyme estimations were made with intestinal homogenates, and occasionally with serum. The first carrier, casein, was found to affect intestinal phosphatase activity, and it was replaced by a non-active carrier, wheat gluten. The results with the two carriers showed essentially the same trend. The elevation of intestinal alkaline phosphatase by dietary fatty acids varied inversely with the chain length for: butyric acid, lauric acid, palmitic acid, and stearic acid. The possibility is considered that decreasing solubility may be associated with the response obtained with these fatty acids. Oleic acid was a more powerful stimulant for the synthesis of intestinal alkaline phosphatase than the saturated fatty acids. Choline, fed simultaneously with oleic acid, completely obliterated the highly significant effect of the acid on both the intestinal and the serum enzyme, These findings indicate that further consideration should be given to the role of phospholipids in absorption of fatty acids. Enzyme response in the serum, studied with a more limited number of fatty acids, lagged a few hours behind the response of the intestinal enzyme.



1955 ◽  
Vol 33 (1) ◽  
pp. 83-88 ◽  
Author(s):  
Nester Dickie ◽  
Margaret I. Robinson ◽  
Jules Tuba

Fasted rats were used in a study of the effect of fatty acids on intestinal and serum phosphatase. Each acid was mixed with a palatable carrier, and then offered to the animals. Enzyme estimations were made with intestinal homogenates, and occasionally with serum. The first carrier, casein, was found to affect intestinal phosphatase activity, and it was replaced by a non-active carrier, wheat gluten. The results with the two carriers showed essentially the same trend. The elevation of intestinal alkaline phosphatase by dietary fatty acids varied inversely with the chain length for: butyric acid, lauric acid, palmitic acid, and stearic acid. The possibility is considered that decreasing solubility may be associated with the response obtained with these fatty acids. Oleic acid was a more powerful stimulant for the synthesis of intestinal alkaline phosphatase than the saturated fatty acids. Choline, fed simultaneously with oleic acid, completely obliterated the highly significant effect of the acid on both the intestinal and the serum enzyme, These findings indicate that further consideration should be given to the role of phospholipids in absorption of fatty acids. Enzyme response in the serum, studied with a more limited number of fatty acids, lagged a few hours behind the response of the intestinal enzyme.





2017 ◽  
Vol 5 (9) ◽  
pp. 450-459
Author(s):  
WalaaAMEl Nahrawy ◽  
◽  
FatmaAAbu Zahra ◽  
RabhaEAEL Dab ◽  
◽  
...  


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