scholarly journals Ultrastructural and molecular characteristics of Setaria species based on sequence analysis of genomic and mitochondrial gene markers in cattle (Bos taurus) and buffaloes (Bubalus bubalis) from Iran

2020 ◽  
Vol 70 (4) ◽  
pp. 1777 ◽  
Author(s):  
A. ALBORZI ◽  
P HADDADMOLAYAN ◽  
M.R. TABANDEH ◽  
M. GHORBANPOOR
Keyword(s):  
Bos Taurus ◽  
Mitochondrial Gene ◽  
Bubalus Bubalis ◽  
Molecular Characteristics ◽  
Scanning Electron Micrographs ◽  
Gene Markers ◽  
Khuzestan Province ◽  
12S Rdna ◽  
Filarial Nematodes ◽  
Setaria Labiatopapillosa

The aim of the present study was to investigate the ultrastructural characteristics and genetic diversity of Setaria parasites from cattle (n=696) and buffalo (n= 522) from Khuzestan province of Iran and to compare them with available data from other countries/regions by sequences analysis of the 12S Rdna and the mitochondrial cytochrome C oxidase subunit I (cox1) genes. Based on SEM (Scanning Electron Micrographs) and light microscopy, all the isolated worms were identified as Setaria labiatopapillosa. Our results showed that 12.3% of cattle were infected with Setaria spp., while no infection was found in buffaloes. The maximal prevalence was observed in cattle younger than one year old. The prevalence rate was not influenced by the season of the year or gender. Comparison of the obtained sequences from Setaria with sequences of Setaria spp. from GenBank confirmed that all samples belong to the species S. labiatopapillosa. The phylogenetic tree constructed using cox1 and 12S rDNA genes of several other filarial nematodes showed that the Khuzestan isolates share a common branch with S. labiatopapillosa from other regions. Intra-specific variation was observed in 12S rDNA but not in cox1. In conclusion, our results indicating that S. labiatopapillosa is the main species involved in the spread of setarial infection in south-west of Iran and the identified worms corresponded mostly to worms that reported previously throughout other continents.

scholarly journals Molecular surveillance and phylogenetic traits of Babesia bigemina and Babesia bovis in cattle (Bos taurus) and water buffaloes (Bubalus bubalis) from Colombia

2018 ◽  
Vol 11 (1) ◽  
Author(s):  
Jeiczon Jaimes-Dueñez ◽  
Omar Triana-Chávez ◽  
Andrés Holguín-Rocha ◽  
Alberto Tobon-Castaño ◽  
Ana M. Mejía-Jaramillo
Keyword(s):  
Bos Taurus ◽  
Bubalus Bubalis ◽  
Babesia Bovis ◽  
Babesia Bigemina ◽  
Molecular Surveillance ◽  
Water Buffaloes

273 OVUM PICKUP AT DIFFERENT TIMES OF A SYNCHRONIZED FOLLICULAR WAVE DID NOT AFFECT IVP IN BOS INDICUS, BOS TAURUS, OR BUBALUS BUBALIS

2010 ◽  
Vol 22 (1) ◽  
pp. 293 ◽  
Author(s):  
L. U. Gimenes ◽  
M. L. Ferraz ◽  
A. Araujo ◽  
P. Fantinato Neto ◽  
M. R. Chiarati ◽  
...  
Keyword(s):  
Mixed Model ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Cumulus Cell ◽  
Bubalus Bubalis ◽  
Genetic Group ◽  
Cleavage Rate ◽  
Follicular Wave ◽  
Counting Data ◽  
Initial Hypothesis

One important factor in the success of ovum pickup (OPU)/IVP in Bos taurus is the follicular status at OPU concerning the dominance period (Hendriksen et al. 2000 Theriogenology 53, 11-20). The hypothesis of the present study is that OPU performed after follicle deviation, when follicles show a mild level of atresia, improves competence for IVP in Nelore (NE), Holstein (HO), and buffaloes (BU). Objectives were to determine effects of OPU done at different times of synchronized follicular wave (1, 3, or 5 d after expected emergence) and of genetic group (NE, HO, and BU) on IVP. A total of 27 heifers (9 of each genetic group) were maintained in contemporary nutritional and environmental conditions during experiment, in a cross-over design, performed in 6 replicates. Recovered oocytes with at least one cumulus cell layer were matured in TCM-199 supplemented with 10% of FCS plus 50 μM of cysteamin and 0.3 mM of cystine, at 38.5°C with 5% CO2 in air for 24 h. IVF was done with 2 × 106 spermatozoa per mL of NE (for bovine oocytes) or BU semen (for BU oocytes), for 20 h at the same incubator conditions of IVM. After IVF, presumptive zygotes were denuded and cultured in SOF under the same previous atmosphere conditions. Medium was changed 3 d after IVF when cleavage rate (CR) was assessed. Blastocyst (BR) and hatching rates (HR) were evaluated 7 and 9 days after IVF, respectively. About 50% of hatched blastocysts were fixed until nuclei counting. Data were analyzed by ANOVA using the Proc Mixed model. No effects of interaction or time of synchronization were observed in any of the variables. Concerning genetic group, NE had better results than HO and BU (mean ± SEM / heifer / replicate), respectively, for visualized follicles (41.0a ± 2.1, 22.1b ± 1.3, 18.8b ± 0.9), total oocytes (37.1a ± 2.5, 15.4b ± 1.2, 14.8b ± 1.0), oocytes at IVM (30.8a ± 2.4, 10.7b ± 1.0, 7.9b ± 0.7), oocytes at IVC (18.7a ± 0.8, 8.0b ± 0.5, 7.5b ± 0.4), cleaved embryos (15.4a ± 0.7, 4.6b ± 0.4, 4.4b ± 0.3),CR(81.8a, 59.1b, 62.3b), blastocysts on Day 7 (5.1a ± 0.6, 1.0b ± 0.2, 0.6b ± 0.1), BR (25.8a, 13.6b, 9.1b), and hatched blastocysts on Day 9 (2.6a ± 0.4, 0.3b ± 0.1, 0.3b ± 0.1). Recovery rate and HR were greater for NE (89.4 and 50.6%, respectively) than for HO (73.3 and 23.2%), but neither differed from BU (82.8 and 31.9%). Also, the percentage of viable was greater for NE (83.0) than for HO (66.9) and BU (53.1). No effects were observed for nuclei counting (NE = 176.6 ± 5.3, HO = 168.9 ± 13.7 and BU = 206.1 ± 23.1). Results demonstrate that Nelore had a better efficiency for IVP than Holstein and buffaloes. OPU performed at different times of synchronized follicular wave did not influence IVP, conversely to the initial hypothesis of this study. FAPESP (06/59550-6, 07/04782-2), Tortuga Cia Zootecnica®, Santa Adele and São Caetano Farms, LMMD, PCAPS, HOVET (Dr. Ubiraem Schalch), VRA, VNP (Prof. Dr. Francisco de Palma Rennó).

scholarly journals Clínica e hematologia de Bos indicus, Bos taurus e Bubalus bubalis inoculados com oocistos de Toxoplasma gondii (Apicomplexa: Toxoplasmatinae)

2001 ◽  
Vol 31 (4) ◽  
pp. 621-626 ◽  
Author(s):  
Francisco Carlos Rodrigues de Oliveira ◽  
Alvimar José da Costa ◽  
Gustavo Adolfo Sabatini
Keyword(s):  
Toxoplasma Gondii ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Bubalus Bubalis

Três animais de cada espécie (Bos indicus, Bos taurus e Bubalus bubalis) foram inoculados, via oral, com 2,0 x 10(5) oocistos de Toxoplasma gondii. Seis outros animais, dois de cada espécie, foram mantidos como testemunhas. As alterações clínicas surgidas a partir do 3º dia após inoculação (DAI) foram: hipertermia, taquicardia, taquipnéia, anorexia, prostração, corrimento nasal e lacrimejamento. Estes sinais foram mais evidentes nos taurinos, espécie que apresentou, ainda, diarréia, fotofobia e conjuntivite. Foi possível isolar T. gondii da corrente sangüínea em todas as espécies. Nos taurinos, a partir do 5º DAI até o final do experimento, o parasito foi isolado de todas as amostras de sangue colhidas semanalmente, com exceção do 14º, 35º e 63º DAI. Os bubalinos apresentaram parasitemia no 7º, 14º, 35º e 70º DAI e os zebuínos apenas no 7º e 28º DAI, correspondendo aos picos de temperatura, em todas as espécies, sendo mais evidente em taurinos. Os parâmetros clínico-laboratoriais demonstraram que os taurinos foram mais sensíveis ao T. gondii do que os zebuínos e estes não diferiram significativamente dos bubalinos, que tiveram aparente normalidade clínico-laboratorial.

Enzymic Profile of the Semen of Bovines (Bubalus bubalis and Bos taurus)

2010 ◽  
Vol 25 (3) ◽  
pp. 222-230 ◽  
Author(s):  
M. S. S. Abdou ◽  
M. M. El-Guindi ◽  
A. A. El-Menoufy ◽  
K. Zaki
Keyword(s):  
Bos Taurus ◽  
Bubalus Bubalis

scholarly journals Diagnosis of Sarcocystis spp. in cattle (Bos taurus) and water buffalo (Bubalus bubalis) in Northern Vietnam

2009 ◽  
Vol 166 (3-4) ◽  
pp. 314-320 ◽  
Author(s):  
C. Jehle ◽  
A. Dinkel ◽  
A. Sander ◽  
M. Morent ◽  
T. Romig ◽  
...  
Keyword(s):  
Water Buffalo ◽  
Bos Taurus ◽  
Bubalus Bubalis ◽  
Northern Vietnam ◽  
Sarcocystis Spp

Admirandus multicavus and Adoncholaimus ussuriensis sp. n. (Nematoda: Enoplida: Oncholaimidae) from the Sea of Japan

Nematology ◽  
2015 ◽  
Vol 17 (10) ◽  
pp. 1229-1244 ◽  
Author(s):  
Vladimir Mordukhovich ◽  
Dmitry Atopkin ◽  
Natalia Fadeeva ◽  
Victoria Yagodina ◽  
Julia Zograf
Keyword(s):  
Sea Of Japan ◽  
Buccal Cavity ◽  
Mitochondrial Gene ◽  
Large Body ◽  
Peter The Great Bay ◽  
The Sea Of Japan ◽  
Scanning Electron Micrographs ◽  
Peter The Great ◽  
Dichotomous Key ◽  
C Subunit

A description of one new, and redescription of one known, species of the subfamily Adoncholaiminae (Nematoda: Oncholaimidae) from Peter the Great Bay (Sea of Japan) are provided. Adoncholaimus ussuriensis sp. n. is characterised by relatively large body size (L = 4.3-5.8 mm in male, 4.4-6.2 mm in female), a pair of terminal pores of the Demanian system opening dorsally at 147-189 μm (2.3-3.0 abd) anterior to the anus, excretory pore located posterior to buccal cavity region, absence of a ventral swelling on tail, long spicules (190-230 μm), presence of a gubernaculum (32-40 μm), presence of a complicated sensory field in the male with a complex supplementary organ and two sets of pilose filaments (5-10 μm long) on the posterior cloacal lobe and four rows of subventral and subdorsal postcloacal sensilla. We provide a redescription of Admirandus multicavus illustrated by light and scanning electron micrographs and propose Adoncholaimus chinensis as a junior synonym of Admirandus multicavus. The diagnoses of both genera are emended. Partial sequences of the mitochondrial gene cytochrome oxidase c subunit I (COI) and D2-D3 region of the 28S rDNA for both species were obtained. A dichotomous key to Adoncholaimus species is provided.

Some biochemical and metabolic aspects of the semen of bovines (Bubalus bubalis and Bos taurus)

2010 ◽  
Vol 94 (1-4) ◽  
pp. 8-15
Author(s):  
M.S.S. Abdou ◽  
M.M. El-Guindi ◽  
A.A. El-Menoufy ◽  
K. Zaki
Keyword(s):  
Bos Taurus ◽  
Bubalus Bubalis
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