273 OVUM PICKUP AT DIFFERENT TIMES OF A SYNCHRONIZED FOLLICULAR WAVE DID NOT AFFECT IVP IN BOS INDICUS, BOS TAURUS, OR BUBALUS BUBALIS

2010 ◽  
Vol 22 (1) ◽  
pp. 293 ◽  
Author(s):  
L. U. Gimenes ◽  
M. L. Ferraz ◽  
A. Araujo ◽  
P. Fantinato Neto ◽  
M. R. Chiarati ◽  
...  
Keyword(s):  
Mixed Model ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Cumulus Cell ◽  
Bubalus Bubalis ◽  
Genetic Group ◽  
Cleavage Rate ◽  
Follicular Wave ◽  
Counting Data ◽  
Initial Hypothesis

One important factor in the success of ovum pickup (OPU)/IVP in Bos taurus is the follicular status at OPU concerning the dominance period (Hendriksen et al. 2000 Theriogenology 53, 11-20). The hypothesis of the present study is that OPU performed after follicle deviation, when follicles show a mild level of atresia, improves competence for IVP in Nelore (NE), Holstein (HO), and buffaloes (BU). Objectives were to determine effects of OPU done at different times of synchronized follicular wave (1, 3, or 5 d after expected emergence) and of genetic group (NE, HO, and BU) on IVP. A total of 27 heifers (9 of each genetic group) were maintained in contemporary nutritional and environmental conditions during experiment, in a cross-over design, performed in 6 replicates. Recovered oocytes with at least one cumulus cell layer were matured in TCM-199 supplemented with 10% of FCS plus 50 μM of cysteamin and 0.3 mM of cystine, at 38.5°C with 5% CO2 in air for 24 h. IVF was done with 2 × 106 spermatozoa per mL of NE (for bovine oocytes) or BU semen (for BU oocytes), for 20 h at the same incubator conditions of IVM. After IVF, presumptive zygotes were denuded and cultured in SOF under the same previous atmosphere conditions. Medium was changed 3 d after IVF when cleavage rate (CR) was assessed. Blastocyst (BR) and hatching rates (HR) were evaluated 7 and 9 days after IVF, respectively. About 50% of hatched blastocysts were fixed until nuclei counting. Data were analyzed by ANOVA using the Proc Mixed model. No effects of interaction or time of synchronization were observed in any of the variables. Concerning genetic group, NE had better results than HO and BU (mean ± SEM / heifer / replicate), respectively, for visualized follicles (41.0a ± 2.1, 22.1b ± 1.3, 18.8b ± 0.9), total oocytes (37.1a ± 2.5, 15.4b ± 1.2, 14.8b ± 1.0), oocytes at IVM (30.8a ± 2.4, 10.7b ± 1.0, 7.9b ± 0.7), oocytes at IVC (18.7a ± 0.8, 8.0b ± 0.5, 7.5b ± 0.4), cleaved embryos (15.4a ± 0.7, 4.6b ± 0.4, 4.4b ± 0.3),CR(81.8a, 59.1b, 62.3b), blastocysts on Day 7 (5.1a ± 0.6, 1.0b ± 0.2, 0.6b ± 0.1), BR (25.8a, 13.6b, 9.1b), and hatched blastocysts on Day 9 (2.6a ± 0.4, 0.3b ± 0.1, 0.3b ± 0.1). Recovery rate and HR were greater for NE (89.4 and 50.6%, respectively) than for HO (73.3 and 23.2%), but neither differed from BU (82.8 and 31.9%). Also, the percentage of viable was greater for NE (83.0) than for HO (66.9) and BU (53.1). No effects were observed for nuclei counting (NE = 176.6 ± 5.3, HO = 168.9 ± 13.7 and BU = 206.1 ± 23.1). Results demonstrate that Nelore had a better efficiency for IVP than Holstein and buffaloes. OPU performed at different times of synchronized follicular wave did not influence IVP, conversely to the initial hypothesis of this study. FAPESP (06/59550-6, 07/04782-2), Tortuga Cia Zootecnica®, Santa Adele and São Caetano Farms, LMMD, PCAPS, HOVET (Dr. Ubiraem Schalch), VRA, VNP (Prof. Dr. Francisco de Palma Rennó).

154 Exosomes in Follicular Fluid Protect the Bovine Oocyte from Heat Shock

2018 ◽  
Vol 30 (1) ◽  
pp. 217 ◽  
Author(s):  
T. A. Rodrigues ◽  
A. Alli ◽  
F. F. Paula-Lopes ◽  
P. Hansen
Keyword(s):  
Heat Shock ◽  
Follicular Fluid ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Cumulus Cell ◽  
Cumulus Cells ◽  
Effect Of Temperature ◽  
Vehicle Group ◽  
Bovine Oocyte ◽  
Cleavage Rate

Elevated temperature can compromise the ability of the mammalian oocyte to develop to the blastocyst stage after fertilization. The microenvironment of the oocyte is determined by the cellular and non-cellular components of the follicle including cumulus cells and follicular fluid. Here we tested whether follicular fluid contains molecules that can protect the bovine oocyte from heat shock during maturation, and if so, whether some of these protective molecules are present in exosomes. The experiments utilised ovaries from Bos taurus and admixtures of B. taurus and Bos indicus. Four separate pools of follicular fluid were prepared by aspiration of follicles from 48 to 70 slaughterhouse ovaries. Exosomes were isolated from follicular fluid by a series of centrifugation, filtration, and ultracentrifugation steps before being reconstituted in PBS. Each of the 4 exosome preparations was subject to particle size and concentration analysis. The experiments were designed as 2 × 3 factorial to test the effect of temperature and supplementation. Cumulus-oocyte complexes (COC) obtained from slaughterhouse ovaries were matured at 38.5°C for 22 h (control) or 41°C for 14 h followed by 38.5°C for 8 h (heat shock). Maturation was performed in the presence of vehicle (PBS), 10% (v/v) follicular fluid, or exosomes (16 × 109 particles/mL). Data were analysed by least-squares ANOVA. Orthogonal contrasts and the mean separation test pdiff were used to compare means. Effects of treatment on cumulus cell expansion (change in diameter after maturation) were replicated 5 times using 119 to 122 COC per treatment. Effects of treatment on embryonic development after fertilization of treated COC was determined in 6 replicates using 244 to 286 embryos per replicate. Expansion was reduced by heat shock (P < 0.001), and affected by treatment (P < 0.05), with both follicular fluid and exosomes preventing the decrease in expansion caused by heat shock. Cleavage was reduced by heat shock (P < 0.001) and affected by treatment (P < 0.05) and the interaction between temperature × supplementation (P < 0.05). Although heat shock reduced the cleavage rate for vehicle-treated oocytes (77 v. 67%), there was no effect of heat shock for oocytes treated with follicular fluid FF (78 v. 74%) or exosomes (79 v. 78%; SEM = 1.4%). Heat shock also reduced the percent of cleaved embryos becoming blastocysts for the vehicle group (27 v. 17%; P < 0.05) but had no effect on percent of cleaved embryos becoming blastocysts for the follicular fluid (31% v. 26%) or exosome groups (28 v. 26%). Uptake of exosomes into isolated cumulus cells and oocytes cultured at 38.5°C for 0.5, 1, 14 and 22 h was examined using labelling of exosomes with 10 µM BODIPY® Ceramide TR (Thermo Fisher Scieintific, Waltham, MA, USA) and confocal microscopy. Exosomes were taken up by cumulus cells after culture for 1 h or later but were not taken up by oocytes. In conclusion, follicular fluid exosomes protected the oocytes from heat shock and this effect seems to be mediated by cumulus cells. Study supported by BARD US-4719-14.

287 EFFECT OF FOLLICULAR WAVE SYNCHRONIZATION AND ABSENCE OF CORPUS LUTEUM ON COC RECOVERY IN Gyr (BOS TAURUS INDICUS) COWS

2010 ◽  
Vol 22 (1) ◽  
pp. 300
Author(s):  
T. Miyauchi ◽  
C. A. C. Fernandes ◽  
E. R. Oliveira ◽  
B. F. L. Alves ◽  
J. H. M. Viana
Keyword(s):  
Bos Taurus ◽  
Animal Health ◽  
Cumulus Cell ◽  
Estradiol Benzoate ◽  
Corpora Lutea ◽  
Portable Ultrasound ◽  
Follicular Wave ◽  
Main Technique ◽  
Oocyte Donors

Transvaginal guided follicle aspiration (TGFA) is the main technique used in Brazil to recover oocytes for in vitro embryo production (IVP) in bovine. Different protocols have been proposed to synchronize follicular emergence in oocyte donors, but most of them were developed for use in European breeds of cattle, which show many differences in ovarian physiology when compared with Zebu breeds. The aim of this study was to compare different protocols for preparation of Gyr (dairy zebu breed) oocyte donors. The TGFA were performed in a donor management facility located in Minas Gerais State, southeast Brazil. Pluriparous cycling Gir cows (n = 42) were used as donors. All cows underwent 3 treatments: G1 (control), no treatment before TGFA; G2, 2 mg of estradiol benzoate (EB) for follicular wave synchronization given i.m. 5 days before TGFA; and G3, norgestomet auricular implants given 9 days and 2 mg of EB plus 0.53 mg of cloprostenol given 5 days before TGFA. The interval between TGFA in the same donor was greater than 30 days. All procedures were made by the same technician, using a portable ultrasound device, disposable 19G or 20G needles, and a vacuum pressure of 80 mm Hg. The aspirated follicular fluid was collected in 50-mL Falcon tubes and sent to the laboratory for COC identification and classification under 50 × magnification. Recovered oocytes were classified according to cumulus cell layers and cytoplasm morphology. The total number of oocytes and viable COC recovered and the procedure length (min), including time spent for TGFA and laboratory manipulation, were compared. Data were evaluated by ANOVA, and means compared by Tukey’s test. A total of 126 TGFA sessions were performed, with recovery of 2,809 oocytes (20.31 ± 12.32 of COC and 14.83 ± 7.97 of viable COC per cow/session; mean ± SD). The total number of recovered oocytes and viable COC recovered were lower in G1 compared with G2 and G3 (15.18 ± 11.07 v. 21.18 ± 9.71 and 24.68 ± 9.03; and 9.53 ± 7.22 v. 16.97 ± 6.47 and 18.84 ± 8.90, respectively; P < 0.05) There was no difference (P > 0.05) between G2 and G3 on the number of oocytes or viable COCs recovered. The procedure length, however, was longer in G1 and G2 compared with G3 (49.6 ± 15.1 and 46.9 ± 13.4 v. 35.8 ± 13.1 min, respectively; P < 0.05) The shorter procedure length in G3 was probably associated with the reduced number of cows showing no corpora lutea (38/42, 90%), which resulted in reduction of bleeding and clot formation in the aspirated fluid. These results show that (1) previous follicular wave synchronization by EB can improve the number and quality of recovered oocytes; (2) the absence of corpora lutea does not increase the number of recovered COC and viable COC, but reduces the time spent in the procedure. Intervet Schering Plough Animal Health.

206 EFFECT OF FOLLICULAR WAVE SYNCHRONIZATION AND SUPERSTIMULATION ON IN VITRO EMBRYO PRODUCTION

2008 ◽  
Vol 20 (1) ◽  
pp. 182 ◽  
Author(s):  
K. Imai ◽  
Y. Inaba ◽  
H. Yoshioka ◽  
Y. Aikawa ◽  
M. Ohtake ◽  
...  
Keyword(s):  
Formation Rate ◽  
Cumulus Cell ◽  
Oocyte Quality ◽  
Annual Conference ◽  
Cleavage Rate ◽  
Embryo Production ◽  
Follicular Wave ◽  
The Mean ◽  
In Vitro Embryo Production

We previously reported that follicular wave synchronization, by removal of the dominant follicle on Day 5 after ovum pickup (OPU), was effective in increasing oocyte quality in the developing follicles (Imai et al. 2006 32th Annual Conference of the IETS, poster presentation no. 277). The current study was designed to examine the effect of superstimulatory treatment to induce subsequent follicular wave synchronization on embryo production by OPU and IVM-IVF-IVC in Holstein dry cows. Cows were reared under the same feeding and environmental conditions, and 2 OPU sessions were conducted in each cow. In the first session, OPU was performed in 8 cows on arbitrary days of the estrous cycle by using a 7.5-MHz linear transducer with needle (Cova needle, Misawa Medical, Tokyo, Japan) connected to an ultrasound scanner (SSD-1200, Aloka, Tokyo, Japan). Follicles larger than 8 mm in diameter were then aspirated and a CIDR was inserted on Day 5 (the day of first OPU session = Day 0). Cows then received 30 mg of FSH (Antrin-R10; Kawasaki Mitaka Pharmaceutical Co., Tokyo, Japan) twice a day from Days 7 to 10 in decreasing doses (6, 6, 4, 4, 3, 3, 2, 2 mg) by i.m. injection. Cloprostenol (PGF; Clopromate C; Sumitomo Pharmaceuticals Co., Tokyo, Japan; 0.75 mg) was administered in the morning of Day 9 (third day of superstimulation). The second OPU session was performed 48 h after PGF administration (Day 11), and only follicles larger than 5 mm in diameter were aspirated. The CIDR was removed from the cows just before OPU. Collected oocytes were evaluated by their cumulus cell morphology, cytoplasmic color, and density. Grades 1 and 2 COC were matured, fertilized, and cultured as described by Imai et al. [2006 J. Reprod. Dev. 52(Suppl.), S19–S29]. Embryo development was assessed by the cleavage rate on Day 2 and by the blastocyst formation rate on Days 7 to 8 (the day of insemination = Day 0). Data were analyzed by Student's t-test. There were no differences in the mean (� SD) number of aspirated follicles or collected oocytes between the first (32.5 � 6.8 and 26.0 � 12.7, respectively) and second (29.3 � 10.4 and 19.0 � 9.4, respectively) OPU sessions (P > 0.1). The percentage of Grade 1 and 2 oocytes for the second OPU session (90.5 � 13.8%) was significantly higher (P < 0.01) than for the first OPU session (63.1 � 6.3%), and significant differences were found for cleavage (79.4 � 14.1, 61.8 � 25.1, P < 0.01) and blastocyst rates (68.1 � 16.7, 24.2 � 22.3, P < 0.001) between sessions. The mean numbers of blastocysts obtained per session were 4.3 � 2.9 and 12.8 � 8.7 in the first and second sessions, respectively (P < 0.01). These results indicate that superstimulatory treatment and subsequent follicular wave synchronization were effective on in vitro embryo production by increasing the oocyte quality.

117 Subclinical Mastitis Reduces Ovulation and Oocyte Quality in Milk-Producing Cows

2018 ◽  
Vol 30 (1) ◽  
pp. 198
Author(s):  
G. Santos ◽  
M. P. Bottino ◽  
M. B. D. Ferreira ◽  
J. C. Silveira ◽  
A. C. F. C. M. Avila ◽  
...  
Keyword(s):  
Follicular Fluid ◽  
Target Genes ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Cumulus Cell ◽  
Cumulus Cells ◽  
Subclinical Mastitis ◽  
Oocyte Quality ◽  
Ovulation Rate ◽  
Follicular Dynamics

The aim was to evaluate the effect of subclinical mastitis by somatic cell count (SCC) on follicular dynamics, ovulation, oocyte and cumulus cell quality, exosome size and concentration in milk-producing cows. Crossbred cows (Bos taurus × Bos indicus; that is, Holstein × Gyr) were randomly allocated to control (SCC <200,000 cells mL−1] and mastitis (SCC >400,000 cells mL−1) groups. In experiment 1 (follicular dynamics), cows (n = 57) were submitted to ultrasonographic evaluations every 24 h, after removal of an intravaginal progesterone device (Day 8) up to Day 10. From Day 10, ultrasound evaluations were performed every 12 h, until ovulation or until 96 h after progesterone device withdrawal, in order to follow final dominant follicle growth and ovulation. In experiment 2 (oocyte, cumulus cells, and follicular fluid evaluation), cows (n = 23) were submitted to follicular aspirations, preceded by synchronization of the emergence of the follicular wave. The levels of target genes in cumulus cells (BCL2, BAX, PI3K, PTEN, FOXO3) were evaluated by RT-qPCR. In the follicular fluid, the exosomes were isolated for evaluation of particle size. Data were analysed by the Glimmix procedure of SAS (SAS Institute Inc., Cary, NC, USA). Ovulation rate (P = 0.09) was higher in control cows [control 77.42% (24/31) and mastitis 57.69% (15/26)]. Viable oocyte rate (P = 0.01) was also higher in control cows [control 59.1% (130/220) and mastitis 41.9% (125/298)]. The dynamics of follicular growth did not differ between groups. The number of degenerate oocytes (P = 0.001) was higher in cows of the mastitis group. In the evaluation of cumulus cell gene expression, there was a higher abundance of BAX transcripts (P = 0.003) in cells of mastitis cows. Additionally, the mean and mode of exosome diameter in mastitis cows were smaller (P = 0.03 and P = 0.02, respectively). In conclusion, ovulation rate, oocyte quality, and follicular fluid exosome diameter were lower in cows with subclinical mastitis, demonstrating a link between mammary gland sanitary status and reproduction.

scholarly journals Clínica e hematologia de Bos indicus, Bos taurus e Bubalus bubalis inoculados com oocistos de Toxoplasma gondii (Apicomplexa: Toxoplasmatinae)

2001 ◽  
Vol 31 (4) ◽  
pp. 621-626 ◽  
Author(s):  
Francisco Carlos Rodrigues de Oliveira ◽  
Alvimar José da Costa ◽  
Gustavo Adolfo Sabatini
Keyword(s):  
Toxoplasma Gondii ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Bubalus Bubalis

Três animais de cada espécie (Bos indicus, Bos taurus e Bubalus bubalis) foram inoculados, via oral, com 2,0 x 10(5) oocistos de Toxoplasma gondii. Seis outros animais, dois de cada espécie, foram mantidos como testemunhas. As alterações clínicas surgidas a partir do 3º dia após inoculação (DAI) foram: hipertermia, taquicardia, taquipnéia, anorexia, prostração, corrimento nasal e lacrimejamento. Estes sinais foram mais evidentes nos taurinos, espécie que apresentou, ainda, diarréia, fotofobia e conjuntivite. Foi possível isolar T. gondii da corrente sangüínea em todas as espécies. Nos taurinos, a partir do 5º DAI até o final do experimento, o parasito foi isolado de todas as amostras de sangue colhidas semanalmente, com exceção do 14º, 35º e 63º DAI. Os bubalinos apresentaram parasitemia no 7º, 14º, 35º e 70º DAI e os zebuínos apenas no 7º e 28º DAI, correspondendo aos picos de temperatura, em todas as espécies, sendo mais evidente em taurinos. Os parâmetros clínico-laboratoriais demonstraram que os taurinos foram mais sensíveis ao T. gondii do que os zebuínos e estes não diferiram significativamente dos bubalinos, que tiveram aparente normalidade clínico-laboratorial.

scholarly journals Follicular dynamic and repeatability of follicular wave development in Peranakan Ongole (PO) cattle

2016 ◽  
Vol 21 (1) ◽  
pp. 26 ◽  
Author(s):  
Muhammad Imron ◽  
Iman Supriatna ◽  
. Amrozi ◽  
Mohamad Agus Setiadi
Keyword(s):  
Bos Taurus ◽  
Research Result ◽  
Bos Indicus ◽  
Follicular Development ◽  
Wave Pattern ◽  
Follicular Wave ◽  
Follicle Diameter ◽  
Wave Patterns ◽  
The Difference ◽  
Follicular Dynamics

<p class="abstrak2">Superovulation treatment on PO cattle (Bos indicus) was less responsive compared to Bos taurus breed. It might due to the difference of their follicular dynamic. This study was conducted to investigate the follicular dynamics and its repeatability in PO cattle. Follicular dynamics observations conducted on 9 cows trough ultrasound scanning every day. Observations of wave patterns repeatability were performed in 6 cows which its wave pattern already known on the next consecutive IOI.  Research result indicated that PO cattle had 3 (66%) and 4-waves (34%) pattern. The first wave of 3 and 4-waves pattern emerged on day -0.4+0.9 and 1.4+1.1 respectively.  The second wave of 3 and 4-wave pattern emerged on day 9.8+1.5 and 7.4+1.9 respectively.  The pattern of 3 waves has a longer follicle dominant duration (11.6+1.5 day) in the first wave of estrous cycle, compared with 4 waves pattern (10+2.92 and 7+1.00 day respectively). The growth rate of dominant follicle was not different significantly between the 3 and 4-waves pattern (0.87+0.23 and 0.94+0.25 mm/day respectively). Similarly, ovulatory follicle diameter between 3 and 4-waves pattern was also not different significantly (12.24+12.34 and 12.30+12.23 mm respectively). Observation of wave patterns repeatability in 6 PO cows indicated that PO cattle had high repeatability in follicular wave pattern (0.88) and the number of growing follicle was 0.91.  This study resulted data for dynamic of follicular development, wave pattern, its repeatability which be expected to design the protocol of superovulation treatment or other reproduction technologies based on follicular dynamic to improve its result in PO cattle.</p><p> </p>

Ovarian Follicular Growth and Development During the Estrous Cycle of Bos taurus, Bos indicus and Bubalus bubalis Heifers Maintained on the Same Nutritional and Environmental Status.

2011 ◽  
Vol 85 (Suppl_1) ◽  
pp. 675-675
Author(s):  
Julia M. Baldrighi ◽  
Manoel Francisco Sa Filho ◽  
Pietro Sampaio Baruselli ◽  
Jose Antonio Visintin ◽  
Mayra Elena Ortiz D'Avila Assumpcao
Keyword(s):  
Estrous Cycle ◽  
Growth And Development ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Bubalus Bubalis ◽  
Follicular Growth ◽  
Environmental Status ◽  
Ovarian Follicular Growth

72 Invitro embryo production using prepubertal calf oocytes with conventional semen and sexed semen ULTRA-4M

2020 ◽  
Vol 32 (2) ◽  
pp. 162
Author(s):  
A. Velazquez ◽  
H. Alvarez ◽  
M. Kjelland ◽  
F. Villaseñor ◽  
G. Ariza ◽  
...  
Keyword(s):  
Bos Taurus ◽  
Bos Indicus ◽  
Sperm Concentration ◽  
Reproductive Potential ◽  
The United States ◽  
Field Trials ◽  
Cleavage Rate ◽  
Embryo Production ◽  
Sexed Semen ◽  
Α Level

Invitro embryo production (IVP) can increase the reproductive potential and genetic quality of cattle, as well as other species. This powerful assisted reproduction tool can be used to produce embryos from prepubertal calves, reducing the generation interval. A recent sexed semen technology known as ULTRA (ST Genetics), completely modified the technique, the media and sperm concentration. In field trials with AI there was no difference between conventional semen (CONV) and ultra-sexed semen at a concentration of 4 million per straw (ULTRA-4M). The combined use of IVP and ULTRA-4M can decrease the selection time for improving dairy and beef cattle herd genetics. The objective of this research was to compare the CONV and ULTRA-4M semen using bovine IVP and prepubertal calves. The research was carried out in the reproduction laboratory of the Facultad de Estudios Superiores Cuautitlán - Universidad Nacional Autónoma de México (FESC-UNAM). The IVP was performed with a continuous invitro culture (IVC) system. The ovaries were collected in Campeche, México, from Bos indicus×Bos taurus crossbred calves (6 months old) using surgical castration (for export to the United States) and transported to the laboratory (FESC-UNAM) in BO-HEPES-IVM (Bioscience™), in an oocyte transporter (WTA). Vitrogen media were used for IVF and IVC. For IVM, the cumulus-oocyte complexes (COCs) were selected (only grades 1 and 2) and matured for 24h at 38.5°C. Matured oocytes (n=600, divided equally into five replicates) were divided into 2 groups, the CONV group and the ULTRA-4M group. The IVF process was conducted with CONV and ULTRA-4M semen from the same bull (Holstein) at a concentration of 2×106 and 0.5×106 spermatozoamL−1, respectively, for 18h in 38.5°C, 5% CO2, 95% air, and 100% humidity. The presumptive zygotes were denuded by pipetting and set in IVC until Day 7 at 38.5°C, 5% CO2, 5% O2, and 90% N2 at 100% humidity. The cleavage results were recorded 56h after the beginning of IVC. The cleavage rate, embryos with more than 6 cells, and blastocysts on Day 7 of culture were evaluated. The statistical analysis was carried out with the GLM procedure of the SAS software (version 9.3; SAS Institute Inc.) to evaluate the results of CONV vs. ULTRA-4M (α level=0.05). The percentage of cleavage for CONV was 46%±1.4 and 43.2%±1.4 for ULTRA-4M. The results for embryos with more than 6 cells in the CONV and ULTRA-4M groups were 16%±0.6 and 14%±0.6, respectively. The percentage of blastocysts on Day 7 for CONV was 9%±0.6 and 8%±0.6 for ULTRA-4M. There were no significant differences between groups (P&gt;0.05) for all variables analysed. In conclusion, under the conditions of this research the ULTRA-4M and CONV produced similar results for IVP.

scholarly journals PSXIV-12 Effect of the addition of canola oil on digestibility, rumen fermentation and methane emissions in beef cattle in the Mexican tropic

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 440-441
Author(s):  
Jonathan Aviles-Nieto ◽  
Claudia Marquez-Mota ◽  
Atmir Romero-Pérez ◽  
José Talamantes-Gómez ◽  
Epigmenio Castillo-Gallegos ◽  
...  
Keyword(s):  
Canola Oil ◽  
Mixed Model ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Latin Square ◽  
Repeated Measure ◽  
Ruminal Fermentation ◽  
Ch4 Emissions ◽  
Fecal Output ◽  
Negative Effect

Abstract An experiment was performed to evaluate the effect of canola oil (CO) supplementation at 4 inclusion rates [CO0=0%, CO2=2%, CO4=4%, and CO6=6% of dry matter intake (DMI)] on digestibility, volatile fat acids (VFA), a mmonia nitrogen (NH3-N), protozoa and methane (CH4) emissions, in cows grazing a tropical grass (Brachiaria sp.). Four cows (Bos taurus × Bos indicus) with rumen cannula (658 ± 92 kg) were randomly assigned to a Latin Square design with 4 treatments and 4 periods (19-d each). Treatments were offered daily at 0600 h and 1500 h together with a commercial concentrate (1% of EBW). Fecal output was measured using an external marker (Cr2O3) dosed once per day for 9 days. Fecal output along with digestibility data were used to estimate DMI. Methane production was monitored twice a day, for 5 consecutive-days, using infrared gas analyzers (Guardian Plus) at the time of CO supplementation. Rumen contents were collected at 0300 h and every 4-h during the last 2-d of each period. Data of digestibility were analyzed as a Latin Square using a mixed model procedure (SAS Inst. Inc., Cary, NC). For ruminal fermentation parameters, collection time was considered a repeated measure. Orthogonal polynomials were performed to evaluate linear and quadratic effects. No effect was observed on fiber digestibility, or any nutrient evaluated with up to 6% CO inclusion. Total protozoa (x104cells/mL), methane emission and acetate molar proportion decreased (P &lt; 0.01) while propionate increased (P &lt; 0.01) with consequent reduction on the acetate:propionate ratio (CO0=3.2, CO2=2.8, CO4=2.6, CO6=2.4; P &lt; 0.01) as the level of CO increased. In conclusion, supplementing CO to animals consuming a tropical forage-based diet, may represent a viable option to reduce CH4 emissions without having a negative effect on the digestibility of the NDF.

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