Microbial Hydroxylation of (±)- and (−)-(2Z,4E)-5-(1',2'-Epoxy-2',6',6'-trimethylcyclohexyl)-3-methyl-2,4-pentadienoic acid into (±)- and (−)-Xanthoxin Acid by Cunninghamella echinulata

2001 ◽  
Vol 65 (4) ◽  
pp. 943-946 ◽  
Author(s):  
Ryou OKAZAKI ◽  
Takayuki ORITANI ◽  
Yoshihiro HARA ◽  
Hirotaka YAMAMOTO
Keyword(s):  
Cunninghamella Echinulata ◽  
Microbial Hydroxylation ◽  
Pentadienoic Acid

Understanding Process Variables and their Interactions for Maximizing Production of Artemisinin Derivative Artemether (Anti-Malarial Drug) Through Cunninghamella echinulata var elegans at 5 L Bioreactor Level

2019 ◽  
Vol 15 (4) ◽  
pp. 442-452
Author(s):  
Kashyap Kumar Dubey ◽  
Punit Kumar
Keyword(s):  
Experimental Finding ◽  
Culture Broth ◽  
Quadratic Model ◽  
Optimum Conditions ◽  
Reverse Phase Hplc ◽  
Polar Solvents ◽  
Reverse Phase ◽  
Experimental Conditions ◽  
Cunninghamella Echinulata ◽  
Life Threatening

Background: Malaria is one of the life threatening diseases which is caused by Plasmodium sp. of protozoa and uses Anopheles mosquitos as vector. Plasmodium vivax and Plasmodium falciparum are common form of malaria parasite. Artemisinin is reported for its antimalarial activities and Artemether which is a methyl ether derivative of Artemisinin, has been found effective against P. falciparum. Methods: In the present study, bioconversion of Artemisinin into Artemether was carried out experimentally and the statistical tools like experimental factorial design and Response Surface Methodology were used to find optimal conditions (concentration of Artemisinin, age of inoculum, temperature & pH) using Cunninghamella echinulata var. elegans. Experimental conditions for maximum product recovery from culture broth were also optimized using various polar and non-polar solvents for extraction. Artemether purity was analyzed by reverse-phase HPLC. Experimental data was fitted in a quadratic model and effect of various parameters was analyzed. Results: It was found that bioconversion of Artemisinin into Artemether is growth associated process. It was observed that molasses used as carbon source supported production of Artemether to 3.4g/L. The biomass and oxygen are key element affecting of bioconversion of Artemisinin into Artemether such as higher dissolved oxygen reduced the Artemether bioconversion. The highest bioconversion of Artemisinin into Artemether was obtained at temperature 25.5oC, 5g/L concentration of Artemisinin, at age of inoculum of 44.5 h and at pH 6.0. Model suggested the highest bioconversion of Artemisinin into Artemether was 54% at shake flask level which was near about experimental finding. An optimal condition for bioconversion was also analyzed and 64% bioconversion was obtained in 5L bioreactor. Conclusion: The outcomes of the study provided optimum conditions for bioconversion of Artemisinin into Artemether.

PRODUÇÃO SIMULTÂNEA DE BIOMASSA E LIPÍDEOS UTILIZANDO MEIOS CONTENDO RESÍDUOS AGROINDUSTRIAIS POR Mucor subtilíssimus UCP/WFCC 1262, Cunninghamella echinulata UCP/WFCC 1299 e Rhizopus microsporus UCP/WFCC 1304 ISOLADOS DO SOLO DA CAATINGA DE PERNAMBUCO

Engevista ◽  
2017 ◽  
Vol 19 (5) ◽  
pp. 1417
Author(s):  
Miller Da Costa Lima ◽  
Tayane de Cássia Dias Mendes Silva ◽  
Adriana Ferreira De Souza ◽  
Marcos Antônio Cavalcanti Luna ◽  
Rosileide Fontenele Silva Andrade ◽  
...  
Keyword(s):  
Rhizopus Microsporus ◽  
Cunninghamella Echinulata

A produção de lipídeos microbianos oferece vantagens em relação aos vegetais e animais, apresentando rápida geração, não necessitando de grandes áreas de terra arável e tendo melhor controle da produção e do produto. Nesse sentido, o objetivo deste trabalho foi realizar uma seleção de fungos mucorales (Mucor subtilíssimus UCP/WFCC 1262, Cunninghamella echinulata UCP/WFCC 1299 e Rhizopus microsporus UCP/WFCC 1304), isolados do solo da Caatinga de Pernambuco em meio contendo resíduos agroindustriais. O cultivo foi realizado em diferentes concentrações de melaço de cana de açúcar, milhocina e pH. A linhagem com elevado potencial em produzir biomassa e lipídeos foi cultivada em diferentes níveis, a partir do meio selecionado, utilizando planejamento fatorial 2³. Os rendimentos das biomassas foram calculados por gravimetria, os lipídeos totais quantifidados após extração por clorofórmio e metanol e a identificação dos ácidos graxos foi realizada por cromatografia gasosa (GC). Adicionalmente, um estudo histoquímico foi realizado com o fungo selecionado. Os resultados obtidos demonstraram que Cunninghamella echinulata foi o micro-organismo selecionado devido a elevada produção simultanea de biomassa (9,05g/L) e lipídeos totais (46,96%) em meio consituído por 10% de melaço de cana de açúcar e 5% de milhocina, com o pH 6. A partir deste meio selecionado foi realizado um planejamento fatorial 2³ para cultivo com a Cunninghella echinulata visando obter a máxima produção de biomassa e lipídeos totais. De acordo com o planejamento o meio constituído por milhocina (8%), melaço de cana de açúcar (12%) e pH 6.0 (ensaio 4), foi obtida a máxima concentração de biomassa (10,1g/L) e lipídeos totais (47,86%).  A partir da biomassa de Cunninghamella echinulata cultivada no meio selecionado do planejamento fatorial foi observado o máximo acúmulo de lipídeo no micélio, confirmando os resultados obtidos neste estudo para a produção de lipídeos.

Microbial hydroxylation of acronycine

1974 ◽  
Vol 17 (6) ◽  
pp. 653-654 ◽  
Author(s):  
D. R. Brannon ◽  
D. R. Horton ◽  
Gordon H. Svoboda
Keyword(s):  
Microbial Hydroxylation

ChemInform Abstract: Microbial Hydroxylation of Some Steroids by Aspergillus wentii MRC 200316.

ChemInform ◽  
2011 ◽  
Vol 42 (11) ◽  
pp. no-no
Author(s):  
Kudret Yildirim
Keyword(s):  
Microbial Hydroxylation ◽  
Aspergillus Wentii

scholarly journals Microbial hydroxylation of progesterone withAcremonium strictum

2003 ◽  
Vol 222 (2) ◽  
pp. 183-186 ◽  
Author(s):  
Mohammad Ali Faramarzi ◽  
Mojtaba Tabatabaei Yazdi ◽  
Mohsen Amini ◽  
Gholamreza Zarrini ◽  
Abbas Shafiee
Keyword(s):  
Microbial Hydroxylation

The Mechanism of the Microbial Hydroxylation of Steroids. Part 2. Hydroxylation of a Δ4-3-Ketosteroid Analog by Rhizopus arrhizus ATCC 11145

1975 ◽  
Vol 53 (14) ◽  
pp. 2041-2044 ◽  
Author(s):  
Herbert L. Holland ◽  
Barbara J. Auret
Keyword(s):  
Transition State ◽  
Rhizopus Arrhizus ◽  
Microbial Hydroxylation ◽  
Microbial C

The Δ4-3-ketosteroid analog (± )-4a-methyl-4,4a,5,6,7,8-hexahydro-2(3H) naphthalenone (1) is hydroxylated at both C-8α and C-8β by Rhizopusarrhizus ATCC 11145. The ratio of C-8α to C-8β hydroxylation is compared with that obtained from the peracid oxidation of 2-ethoxy-4a-methyl-3,4,4a,5,6,7-hexahydronaphthalene (4) and a mechanism is proposed for both microbial and chemical hydroxylation in which the stereochemistry of the product is transition state controlled. The significance of this to the mechanism of microbial C-6β hydroxylation of Δ4-3-ketosteroids is discussed.

Cunninghamella echinulata. [Descriptions of Fungi and Bacteria].

1966 ◽  
Author(s):  
A. K. Sarbhoy
Keyword(s):  
Geographical Distribution ◽  
World Wide ◽  
Cucurbita Pepo ◽  
Cucurbita Maxima ◽  
Tropical Countries ◽  
Cunninghamella Echinulata

Abstract A description is provided for Cunninghamella echinulata. Information is included on the disease caused by the organism, its transmission, geographical distribution, and hosts. HOSTS: On decaying flowers of Cucurbita pepo, dung and soil. DISEASE: Reported pathogenic to Spanish pumpkin (Cucurbita maxima) at Chandigarh and neighbouring villages in the Punjab, India by Grover (1965). Symptoms recognizable when the flowers begin to die off gradually. Diseased flowers do not open properly. Infected flowers are covered with the cottony mycelium of the pathogen and infected fruits may become detached from the plant and continue to rot on the soil. Leaves and stalks are also frequently infected. GEOGRAPHICAL DISTRIBUTION: World-wide (mostly tropical countries). TRANSMISSION: Air borne; may survive in fragments of mummified pumpkin fruit up to 32 months.

Microbial hydroxylation of steroids. 6. Hydroxylation of C-6-substituted androst-4-ene-3,17-diones by Rhizopusarrhizus ATCC 11145

1979 ◽  
Vol 57 (13) ◽  
pp. 1585-1587 ◽  
Author(s):  
Herbert L. Holland ◽  
Peter R. P. Diakow
Keyword(s):  
Microbial Hydroxylation ◽  
Enol Acetates

The products arising from the incubations of C-6α and C-6β chloro-, fluoro-, and methyl-substituted Δ4-3-keto steroids with the C-6β hydroxylating fungus Rhizopusarrhizus ATCC 11145 have been identified, and their formation rationalised in terms of Δ3,5 enolic intermediates. The incubations of C-6 chloro and methyl Δ3,5 enol acetates with R. arrhizus, and the oxidations by m-chloroperoxybenzoic acid of the corresponding enol ethyl ethers have also been described. The relevance of the results so obtained to the mechanism of C-6β hydroxylation of Δ4-3-keto steroids by R. arrhizus is discussed.

Susceptibility to peroxidation of the major mycelial lipids of Cunninghamella echinulata

2008 ◽  
Vol 110 (11) ◽  
pp. 1062-1067 ◽  
Author(s):  
Stylianos Fakas ◽  
Ioannis Papapostolou ◽  
Seraphim Papanikolaou ◽  
Christos D. Georgiou ◽  
George Aggelis
Keyword(s):  
Cunninghamella Echinulata
Sign in / Sign up

Export Citation Format

Share Document