The Performance of Strep B Carrot Broth™ versus Latex Agglutination Test for Rapid Detection of Group B Streptococcus Colonization Status and Its Prevalence in Near - Term Pregnant Women

2018 ◽  
Vol 27 (4) ◽  
pp. 7-11
Author(s):  
Abdelraouf , Nada S. ◽  
Khattab , Mona A. ◽  
Atallab , Makram F. ◽  
Abd El Rahman , Rehab M.
Keyword(s):  
Pregnant Women ◽  
Rapid Detection ◽  
Group B Streptococcus ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Latex Agglutination Test ◽  
Near Term ◽  
Group B ◽  
Colonization Status

scholarly journals Evaluation of chromogenic medium and direct latex agglutination test for detection of group B streptococcus in vaginal specimens from pregnant women in Lebanon and Kuwait

2014 ◽  
Vol 63 (10) ◽  
pp. 1395-1399 ◽  
Author(s):  
Nahed Ghaddar ◽  
Wadha Alfouzan ◽  
Elie Anastasiadis ◽  
Tamima Al Jiser ◽  
Saad Eddine Itani ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Group B Streptococcus ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Latex Agglutination Test ◽  
Chromogenic Medium ◽  
Predictive Values ◽  
Vaginal Swabs ◽  
Chromogenic Agar ◽  
Group B

This study was undertaken to evaluate chromogenic medium and a direct latex agglutination test (DLA) for detection of Group B Streptococcus (GBS) in the vaginal specimens of pregnant women, and to ascertain the prevalence of GBS in this population in Kuwait and Lebanon. Vaginal swabs, collected from women at 35–37 weeks of gestation, were cultured on 5 % sheep blood agar (SBA), colistin nalidixic acid agar (CNA), Strept B Select chromogenic agar (SBS) as well as Lim enrichment broth in 168 cases in Lebanon while only SBA was used for 1391 samples in Kuwait. In addition, vaginal samples from 102 GBS-positive and 20 GBS-negative women near the time of delivery were collected in Kuwait for evaluation of the DLA test. During the study period, the prevalence of GBS colonization was determined to be 20.7 % (288/1391) in Kuwait while 18.4 % (31) of 168 pregnant women in Lebanon had vaginal cultures positive for GBS. By direct plating of vaginal swabs on the three media used, the isolation rates of GBS were 51.6, 64.5 and 77.4 % on SBA, CNA and SBS, respectively, which increased to 90.35, 93.1 and 96.8 %, respectively, following subculture in Lim broth after 18 h of incubation. The sensitivity of the DLA test was found to be dependent on the density of GBS colonization, resulting in 100 % sensitivity and 100 % specificity for heavy (>102 c.f.u. per swab) and moderately heavy (50–100 c.f.u. per swab) growth of GBS. However, for vaginal specimens yielding <50 c.f.u. per swab, the sensitivity, specificity, positive and negative predictive values of the DLA test were 100, 55.5, 63.6 and 100 %, respectively. In conclusion, a chromogenic agar, such as SBS, and a DLA test can be used for rapid detection of GBS in pregnant women. The DLA test, in particular, could prove to be a useful tool for immediate detection of GBS in women near delivery so that intrapartum antibiotic prophylaxis can be initiated.

scholarly journals Latex Agglutination Test for Early Detection of Causative Organism in Acute Bacterial Meningitis

2013 ◽  
Vol 33 (1) ◽  
pp. 34-38
Author(s):  
B Mishra ◽  
C Mahaseth ◽  
A Rayamajhi
Keyword(s):  
Cerebrospinal Fluid ◽  
Bacterial Meningitis ◽  
Group B Streptococcus ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Latex Agglutination Test ◽  
Acute Bacterial Meningitis ◽  
Causative Organism ◽  
Hemophilus Influenzae Type B ◽  
Group B

Introduction: Acute bacterial meningitis is one of the leading causes of mortality and morbidity in children. Identification of the causative organism is crucial to its management and outcome. The objective of this study was to see the usefulness of latex agglutination test in the early diagnosis of acute bacterial meningitis. Materials and Methods: A hospital based prospective cross-sectional study was conducted at Kanti Children’s Hospital during December 2004 to August 2005. Cerebrospinal fluid from 150 consecutive clinically suspected cases of acute bacterial meningitis between the age group of 2 months to 14 years were analyzed. Bacterial culture and latex agglutination test was done on cerebrospinal fluid obtained from all 150 suspected cases of acute bacterial meningitis. Latex agglutination test was done using the BD DirectigenTM Meningitis Combo test kit (Becton, Dickinson and company, USA) for Streptococcus pneumoniae, group B Streptococcus, Escherichia coli, Neisseria meningitidis group A,C and Y/ W135, and Hemophilus influenzae type b. Data was analysed by using SPSS Version 11.5. Results: Of the 150 Cerebrospinal fluid samples analysed bacterial culture identified only 4 meningitis cases giving an isolation rate of 1.3% whereas latex agglutination test identified 29 cases giving an isolation rate of 19.3% from 150 samples. Streptococcus pneumoniae, Hemophilus influenzae type b and Group B Streptococcus were the most common causative organism. Conclusion: Latex agglutination test has a better yield, higher sensitivity, provides microbiological diagnosis earlier than the traditional cerebrospinal fluid culture and is easy to perform. DOI: http://dx.doi.org/10.3126/jnps.v33i1.7047 J Nepal Paediatr Soc. 2013;33(1):34-38

scholarly journals Evaluation of StrepB Carrot Broth versus Lim Broth for Detection of Group B Streptococcus Colonization Status of Near-Term Pregnant Women

2008 ◽  
Vol 46 (8) ◽  
pp. 2780-2782 ◽  
Author(s):  
D. L. Church ◽  
H. Baxter ◽  
T. Lloyd ◽  
B. Miller ◽  
S. Elsayed
Keyword(s):  
Pregnant Women ◽  
Group B Streptococcus ◽  
Near Term ◽  
Group B ◽  
Colonization Status

scholarly journals Development of latex agglutination test for rapid detection of antibodies againstBovine herpesvirus 1andBovine herpesvirus 5in cattle

2012 ◽  
Vol 24 (6) ◽  
pp. 1162-1165 ◽  
Author(s):  
Qiang Fan ◽  
Lan Yao ◽  
Ming Ding ◽  
Dan-Jing Wang ◽  
Huan-Chun Chen ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Latex Agglutination Test

scholarly journals Group B Streptococcus Recto-Vaginal Colonization in Near-Term Pregnant Women, Southwest Ethiopia

2020 ◽  
Vol 30 (5) ◽  
Author(s):  
Woubishet Girma ◽  
Nadia Yimer ◽  
Tesfaye Kassa ◽  
Elias Yesuf
Keyword(s):  
Pregnant Women ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Group B Streptococcus ◽  
Susceptibility Pattern ◽  
Southwest Ethiopia ◽  
Vaginal Colonization ◽  
History Of ◽  
Near Term ◽  
Group B

BACKGROUND: Group B Streptococcus (GBS) is recognized as an important cause of maternal and neonatal morbidity and mortality. Maternal vaginal carriage of GBS (Streptococcus agalactiae) can lead to vertical transmission to the neonate at the time of delivery. However, little is known about its prevalence, predictors and antibiotic susceptibility pattern in Jimma, Ethiopia. This study assessed the prevalence, antimicrobial susceptibility pattern and determinants of GBS recto-vaginal colonization among near-term pregnant women.METHODS: A cross-sectional study was conducted from May to August 2015 at Jimma University Medical Centre in Southwest Ethiopia. Data through questionnaire and GBS isolates from vaginal and rectal swabs were collected. Antimicrobial susceptibility testing was performed.RESULTS: The overall prevalence of GBS colonization among near term pregnant women (35-37 weeks) was 16.3% (22/135). The majority of GBS isolates were sensitive to Ampicillin and Penicillin G with 95.5% and 90.1%, respectively. Erythromycin and clindamycin were resisted by 50% and 40.9% of the isolates, respectively, whereas gentamicin was resisted by all isolates. GBS colonization was significantly associated with a history of preterm delivery (PTD) (AOR: 6.3, 95% CI: 1.42, 28.3) and history of urinary tract infection (UTI) during current pregnancy (AOR: 6.4, 95% CI, 1.95, 21.1).CONCLUSION: Our study indicated that one among six near-term pregnant women had recto-vaginal GBS colonization. In places where universal screening is not feasible, selective screening for factors particularly history of PTD and UTI during current pregnancy may be a reasonable option. Antibiotic susceptibility testing should be performed while using Erythromycin, Clindamycin or Gentamicin. 

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