scholarly journals A Cytolethal Distending Toxin Gene-Based Multiplex PCR Assay for Detection of Campylobacter spp. in Stool Specimens and Comparison with Culture Method

2012 ◽  
Vol 74 (7) ◽  
pp. 857-862 ◽  
Author(s):  
Sachi SHIRAMARU ◽  
Masahiro ASAKURA ◽  
Haruna INOUE ◽  
Akira NAGITA ◽  
Akio MATSUHISA ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Culture Method ◽  
Toxin Gene ◽  
Cytolethal Distending Toxin ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
Stool Specimens ◽  
Campylobacter Spp

scholarly journals A Cytolethal Distending Toxin Gene-Based Multiplex PCR Assay for Campylobacter jejuni, C. fetus, C. coli, C. upsaliensis, C. hyointestinalis, and C. lari

2016 ◽  
Vol 69 (3) ◽  
pp. 256-258 ◽  
Author(s):  
Kazumasa Kamei ◽  
Hiroki Kawabata ◽  
Masahiro Asakura ◽  
Worada Samosornsuk ◽  
Atsushi Hinenoya ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Campylobacter Jejuni ◽  
Toxin Gene ◽  
Cytolethal Distending Toxin ◽  
Pcr Assay ◽  
Multiplex Pcr Assay

scholarly journals Multiplex PCR Assay for Identifi cation and Differentiation of Campylobacter jejuni and Campylobacter coli Isolates

Folia Medica ◽  
2016 ◽  
Vol 58 (2) ◽  
pp. 95-100 ◽  
Author(s):  
Maria R. Pavlova ◽  
Elina G. Dobreva ◽  
Katucha I. Ivanova ◽  
Galina D. Asseva ◽  
Ivan N. Ivanov ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Campylobacter Jejuni ◽  
Accurate Method ◽  
Clinical Isolates ◽  
Campylobacter Coli ◽  
Pcr Assay ◽  
Biochemical Tests ◽  
Multiplex Pcr Assay ◽  
Hydrolysis Of ◽  
Campylobacter Spp

AbstractIntroduction: Campylobacter spp. are important causative agents of gastrointestinal infections in humans. The most frequently isolated strains of this bacterial genus are Campylobacter jejuni and Campylobacter coli. To date, genetic methods for bacterial identification have not been used in Bulgaria. We optimized the multiplex PSR assay to identify Campylobacter spp. and differentiate C. jejuni from C. coli in clinical isolates. We also compared this method with the routinely used biochemical methods.Aim: To identify Campylobacter spp. and discriminate C. coli from C. jejuni in clinical isolates using multiplex PCR assay.Materials and methods: Between February 2014 and January 2015 we studied 93 stool samples taken from patients with diarrheal syndrome and identified 40 species of Campylobacter spp. in them. The clinical material was cultured in microaerophilic atmosphere, the isolated strains being biochemically diff erentiated (hydrolysis of sodium hippurate for C. jejuni, and hydrolysis of indoxyl acetate for C. coli). DNA was isolated from the strains using QiaAmp MiniKit (QIAGEN, Germany). Twenty strains were tested with multiplex PCR for the presence of these genes: cadF, characteristic for Campylobacter spp., hipO for C. jejuni and asp for C. coli.Results and discussion: The biochemical tests identified 16 strains of C. jejuni, 3 strains of C. coli, and 1 strain of C. upsaliensis. After the multiplex PCR assay the capillary gel electrophoresis confirmed 16 strains of C. jejuni, 2 strains of C. coli and 2 strains of Campylobacter spp. - because of the presence of the gene cadF. C. jejuni has the gene hipO, and it is possible that this gene may not be expressed in the biochemical differentiation yielding a negative reaction as a result. In comparison, we can conclude that the genetic differentiation is a more accurate method than the biochemical tests.Conclusion: The multiplex PCR assay is a fast, accurate method for identifi cation of Campylobacter spp. which makes it quite necessary in the clinical diagnostic practice.

scholarly journals Development of a Single-Reaction Multiplex PCR Toxin Typing Assay for Staphylococcus aureusStrains

2000 ◽  
Vol 66 (4) ◽  
pp. 1347-1353 ◽  
Author(s):  
Naresh K. Sharma ◽  
Catherine E. D. Rees ◽  
Christine E. R. Dodd
Keyword(s):  
Multiplex Pcr ◽  
Toxin Gene ◽  
Gene Products ◽  
Pcr Assay ◽  
Specific Primers ◽  
Purification Method ◽  
Multiplex Pcr Assay ◽  
Enterotoxin Genes ◽  
Immunological Tests ◽  
Single Reaction

ABSTRACT We describe here the development of a single-reaction multiplex PCR assay for the enterotoxin genes from Staphylococcus aureusthat utilizes a universal toxin gene primer in combination with toxin-specific primers to amplify characteristic toxin gene products. In combination with a new DNA purification method, the assay can detect enterotoxin genes A to E from a pure culture within 3 to 4 h. The test was used to characterize a diverse set of environmental S. aureus isolates, and a 99% correlation with toxin typing using standard immunological tests was found. The design of the assay allows it to be extended to include both newly characterized and as-yet-unknown toxin genes.

Isolation, identification and differentiation of Campylobacter spp. using multiplex PCR assay from goats in Khartoum State, Sudan

2017 ◽  
Vol 49 (3) ◽  
pp. 575-581 ◽  
Author(s):  
Atif Elbrissi ◽  
Y. A. Sabeil ◽  
Khalda A. Khalifa ◽  
Khalid Enan ◽  
Osama M. Khair ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
Campylobacter Spp

scholarly journals Evaluation of a Cytolethal Distending Toxin (cdt) Gene-Based Species-Specific Multiplex PCR Assay for the Identification ofCampylobacterStrains Isolated from Poultry in Thailand

2007 ◽  
Vol 51 (9) ◽  
pp. 909-917 ◽  
Author(s):  
Worada Samosornsuk ◽  
Masahiro Asakura ◽  
Emi Yoshida ◽  
Takashi Taguchi ◽  
Kazuhiko Nishimura ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Cytolethal Distending Toxin ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
Species Specific

A Novel Multiplex PCR Assay for Genotyping of Clostridium difficile from Stool Specimens

2009 ◽  
Vol 104 ◽  
pp. S183-S184
Author(s):  
Xiangwen Meng ◽  
Hongjun Zhang ◽  
Thomas Chen ◽  
Tat-Kin Tsang
Keyword(s):  
Clostridium Difficile ◽  
Multiplex Pcr ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
Stool Specimens
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