scholarly journals Evaluation of a new multiplex PCR assay (ParaGENIE G-Amoeba Real-Time PCR kit) targeting Giardia intestinalis, Entamoeba histolytica and Entamoeba dispar/Entamoeba moshkovskii from stool specimens: evidence for the limited performances of microscopy-based approach for amoeba species identification

2018 ◽  
Vol 24 (11) ◽  
pp. 1205-1209 ◽  
Author(s):  
F. Morio ◽  
S. Valot ◽  
A. Laude ◽  
G. Desoubeaux ◽  
N. Argy ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Real Time ◽  
Species Identification ◽  
Real Time Pcr ◽  
Giardia Intestinalis ◽  
Pcr Assay ◽  
Entamoeba Dispar ◽  
Multiplex Pcr Assay ◽  
Stool Specimens ◽  
Entamoeba Moshkovskii

Species Identification of Fox-, Mink-, Dog-, and Rabbit-Derived Ingredients by Multiplex PCR and Real-Time PCR Assay

2017 ◽  
Vol 185 (1) ◽  
pp. 1-12 ◽  
Author(s):  
Qingqing Wu ◽  
Shengnan Xiang ◽  
Wenjun Wang ◽  
Jinyan Zhao ◽  
Jinhua Xia ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Real Time ◽  
Species Identification ◽  
Real Time Pcr ◽  
Pcr Assay

Development of a Multiplex Real-Time PCR Assay with Internal Amplification Control for the Detection of Shigella Species and Enteroinvasive Escherichia coli

2010 ◽  
Vol 73 (9) ◽  
pp. 1618-1625 ◽  
Author(s):  
DEANNE M. DEER ◽  
KEITH A. LAMPEL
Keyword(s):  
Escherichia Coli ◽  
Multiplex Pcr ◽  
Real Time ◽  
Real Time Pcr ◽  
The United States ◽  
Internal Amplification Control ◽  
Control Strain ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
Shigella Species

Shigella species, particularly S. sonnei and S. flexneri, remain some of the leading bacterial etiological agents of gastrointestinal diseases in the United States and globally. The isolation and detection of these foodborne pathogens are critical for preventing the spread of disease and facilitating epidemiological investigations aimed at determining the source of a Shigella infection outbreak. A multiplex real-time PCR-based assay was developed that targets all four species of Shigella plus enteroinvasive Escherichia coli. The assay incorporates primers directed to the ipaH genes located on both the virulence plasmid and chromosome, the plasmid-encoded virulence gene mxiC, a mutated mxiC gene (mxiC::kan) that differentiates wild-type strains from a laboratory control strain, and an internal amplification control. More than 50 isolates of all four Shigella species were tested for inclusivity and specificity of the multiplex PCR assay, and more than 30 non-Shigella isolates were tested for exclusivity of the assay. The sensitivity of the assay was 1 to 3 CFU and 5 to 50 fg of target (total) DNA for the ipaH, mxiC, and mxiC::kan gene targets. The assay performed equally well and with no measurable inhibition in the Shigella target reactions when rinsates of several high-risk produce commodities (parsley, cilantro, alfalfa sprouts, and lettuce) were added to the reactions. This multiplex PCR assay is sensitive and specific and has the added dimension of discriminating all Shigella species from the positive control strain so that in any sample analysis other strains can be excluded as a source of contamination.

scholarly journals Multiplex PCR assay for species identification of bovine mastitis pathogens

2011 ◽  
Vol 111 (6) ◽  
pp. 1349-1356 ◽  
Author(s):  
B.R. Shome ◽  
S. Das Mitra ◽  
M. Bhuvana ◽  
N. Krithiga ◽  
D. Velu ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Species Identification ◽  
Bovine Mastitis ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
Mastitis Pathogens

scholarly journals Multiplex PCR assay for animal species identification in meat bone meal

2019 ◽  
Vol 387 ◽  
pp. 012018
Author(s):  
M Cahyadi ◽  
T Wibowo ◽  
Y P Nugraheni ◽  
A Fadhila ◽  
A Pramono ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Species Identification ◽  
Animal Species ◽  
Bone Meal ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
Meat Bone Meal
Sign in / Sign up

Export Citation Format

Share Document