J022055 Influence of Vascular Shape Extraction Method and Non-Newtonian Hemodynamic Behavior on WSS Distribution Estimation on Cerebral Aneurysm in MR Based CFD

2012 ◽  
Vol 2012 (0) ◽  
pp. _J022055-1-_J022055-5
Author(s):  
Kohei AOKI ◽  
Yuki ONISHI ◽  
Kenji AMAYA ◽  
Toshiyasu SHIMIZU ◽  
Haruo ISODA ◽  
...  
Author(s):  
Douglas C. Barker

A number of satisfactory methods are available for the electron microscopy of nicleic acids. These methods concentrated on fragments of nuclear, viral and mitochondrial DNA less than 50 megadaltons, on denaturation and heteroduplex mapping (Davies et al 1971) or on the interaction between proteins and DNA (Brack and Delain 1975). Less attention has been paid to the experimental criteria necessary for spreading and visualisation by dark field electron microscopy of large intact issociations of DNA. This communication will report on those criteria in relation to the ultrastructure of the (approx. 1 x 10-14g) DNA component of the kinetoplast from Trypanosomes. An extraction method has been developed to eliminate native endonucleases and nuclear contamination and to isolate the kinetoplast DNA (KDNA) as a compact network of high molecular weight. In collaboration with Dr. Ch. Brack (Basel [nstitute of Immunology), we studied the conditions necessary to prepare this KDNA Tor dark field electron microscopy using the microdrop spreading technique.


2007 ◽  
Vol 3 (10) ◽  
pp. 1-12
Author(s):  
DOUG BRUNK
Keyword(s):  

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
JR Tormo ◽  
N Tabanera ◽  
D Conway ◽  
P Ramos ◽  
A Redondo ◽  
...  

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