Improvement of Bacterial Blight Resistance in Rice Cultivars Jyothi and IR50 via Marker-Assisted Backcross Breeding

AbstractHybrid rice has contributed greatly to the self-sufficiency of the food supply in China. However, bacterial blight is a major disease that limits hybrid rice production in China. The study was conducted to develop an efficient breeding technique to improve the bacterial blight resistance in hybrid rice. A marker-assisted backcross breeding technique was adopted to improve HN189, an elite restorer line containing the Pi1 gene. This breeding technique was simplified to foreground selection with only one generation of backcrossing and background selection based on phenotypic selection. A novel bacterial blight resistance gene, Xa23, was introgressed into HN189. Two improved restorer lines, HBH145 (with one generation of backcrossing) and HBH146 (with two generations of backcrossing), were obtained that had a significant bacterial blight resistance advantage over HN189. The corresponding hybrid combination Tianyou H145 (Tianfeng A / HBH145) was certified one year earlier than Qianyou H146 (Qianjiang 1A / HBH146). The use of the marker-assisted backcross breeding technique with one generation of backcrossing and without background selection in rice breeding programs shortened the breeding period of the rice.

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Basmati rice lines development carrying multiple bacterial blight resistance genes pyramided using the marker-assisted backcross breeding approach

Molecular Breeding ◽

10.1007/s11032-019-1047-7 ◽

2019 ◽

Vol 39 (10-11) ◽

Cited By ~ 4

Author(s):

Muhammad Sabar ◽

Muhammad Akhter ◽

Tahira Bibi ◽

Awais Riaz ◽

Zulqarnain Haider ◽

...

Keyword(s):

Resistance Genes ◽

Bacterial Blight ◽

Basmati Rice ◽

Blight Resistance ◽

Bacterial Blight Resistance ◽

Backcross Breeding ◽

Marker Assisted Backcross Breeding

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Improvement of bacterial blight resistance of the popular variety, Nellore Mahsuri, NLR34449 through marker-assisted breeding

10.21203/rs.3.rs-66828/v1 ◽

2020 ◽

Author(s):

Aleena D ◽

Padma V ◽

Rekha G ◽

Dilip T ◽

Punniakotti E ◽

...

Keyword(s):

Resistance Genes ◽

Bacterial Blight ◽

Grain Quality ◽

Rice Variety ◽

Blight Resistance ◽

Bacterial Blight Resistance ◽

Marker Assisted Breeding ◽

Fine Grain ◽

High Level ◽

Marker Assisted Backcross Breeding

Abstract To combat the dreaded diseases in rice like bacterial blight and blast, host plant resistance has been advocated as a sustainable method. Through the present study, we have successfully incorporated three major bacterial blight (BB) resistance genes viz., Xa21, xa13 and xa5 into NLR3449, a high yielding, blast resistant, fine-grain type popular rice variety through marker-assisted backcross breeding. Foreground selection was carried out using PCR based, gene-specific markers viz., pTA248 (Xa21), xa13prom (xa13) and xa5FM (xa5) at each generation of backcrossing, while 127 polymorphic SSR markers spanning on 12 chromosomes were used for background selection and backcrossing was limited to two rounds. At BC2F1 generation, a single plant (NLR-87-10) with 89.9% recovery and possessing all the three bacterial blight resistance genes was forwarded to BC2F2 generation. A solitary BC2F2 plant viz., NLR-87-10-106 possessing all the three resistance genes and > 90% genome recovery was identified and advanced through selfing till BC2F4 generation by adopting pedigree method. Three best lines at BC2F4 lines, possessing high level of resistance against bacterial blight and blast and equivalent or superior to NLR 34449 in terms of yield, grain quality and agro-morphological traits have been identified and advanced for multi-location trials.

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Dominance Reversal of a Bacterial Blight Resistance Gene in Some Rice Cultivars

Phytopathology ◽

10.1094/phyto-68-461 ◽

1978 ◽

Vol 68 (3) ◽

pp. 461 ◽

Cited By ~ 20

Author(s):

G. S. Sidhu

Keyword(s):

Resistance Gene ◽

Bacterial Blight ◽

Blight Resistance ◽

Rice Cultivars ◽

Bacterial Blight Resistance ◽

Bacterial Blight Resistance Gene

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Incorporation of Bacterial Blight Resistance Genes Into Lowland Rice Cultivar Through Marker-Assisted Backcross Breeding

Phytopathology ◽

10.1094/phyto-09-15-0226-r ◽

2016 ◽

Vol 106 (7) ◽

pp. 710-718 ◽

Cited By ~ 6

Author(s):

Sharat Kumar Pradhan ◽

Deepak Kumar Nayak ◽

Elssa Pandit ◽

Lambodar Behera ◽

Annamalai Anandan ◽

...

Keyword(s):

Resistance Gene ◽

Resistance Genes ◽

Bacterial Blight ◽

Target Genes ◽

Rice Cultivar ◽

Recurrent Parent ◽

Bacterial Blight Resistance ◽

Backcross Breeding ◽

Marker Assisted Backcross Breeding ◽

Recipient Parent

Bacterial blight (BB) of rice caused by Xanthomonas oryzae pv. oryzae is a major disease of rice in many rice growing countries. Pyramided lines carrying two BB resistance gene combinations (Xa21+xa13 and Xa21+xa5) were developed in a lowland cultivar Jalmagna background through backcross breeding by integrating molecular markers. In each backcross generation, markers closely linked to the disease resistance genes were used to select plants possessing the target genes. Background selection was continued in those plants carrying resistant genes until BC3 generation. Plants having the maximum contribution from the recurrent parent genome were selected in each generation and hybridized with the recipient parent. The BB-pyramided line having the maximum recipient parent genome recovery of 95% was selected among BC3F1 plants and selfed to isolate homozygous BC3F2 plants with different combinations of BB resistance genes. Twenty pyramided lines with two resistance gene combinations exhibited high levels of tolerance against the BB pathogen. In order to confirm the resistance, the pyramided lines were inoculated with different X. oryzae pv. oryzae strains of Odisha for bioassay. The genotypes with combination of two BB resistance genes conferred high levels of resistance to the predominant X. oryzae pv. oryzae isolates prevalent in the region. The pyramided lines showed similarity with the recipient parent with respect to major agro-morphologic traits.

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