scholarly journals Chromosomal Aberrations Induced by Carbaryl in Root Meristem Cells of Pisum Sativum L.

2017 ◽  
Vol 14 (3) ◽  
pp. 985-987
Author(s):  
Khushboo Kushwah ◽  
Ravendra Singh Chauhan ◽  
R.K. Sarbhoy ◽  
Harshal Kumar
Keyword(s):  
Cell Division ◽  
Pisum Sativum ◽  
Somatic Cell ◽  
Chromosomal Aberrations ◽  
Filter Paper ◽  
Root Meristem ◽  
Distilled Water ◽  
Root Tips ◽  
Uniform Size ◽  
Pisum Sativum L

ABSTRACT: Carbaryl, which is also known as sevin, induced mitostatic and turbagenic leading to clastogenic effects in the root meristem cells of Pisum sativum. The study was conducted at Department of Botany, Agra College, Agra. Seeds of uniform size of Pisum sativum were germinated on moist filter paper in petriplates. 1to 2 mm root tips were cut and treated with different concentrations (0.1, 0.2, 0.3,0.5%) of carbaryl prepared in distilled water for varying duration (3 to 9 hrs.) of time. It has mitodepressive and mitostatic effects on somatic cell division. These effects are directly proportional to concentration and duration. Common clastogenic effects are stickiness, condensation, breakage and bridges etc. Present investigation clearly revealed that carbaryl showed clastogenic and mitostatic effects. So, it should be used with precautions as it can be hazardous to both targeted and non-targeted biota.

Protective roles of exogenous polyamines on chromosomal aberrations in Hordeum vulgare exposed to salinity

Biologia ◽  
2010 ◽  
Vol 65 (6) ◽  
Author(s):  
Selma Tabur ◽  
Kıymet Demir
Keyword(s):  
Cell Division ◽  
Hordeum Vulgare ◽  
Mitotic Activity ◽  
Chromosomal Aberrations ◽  
Root Meristem ◽  
Control Group ◽  
Distilled Water ◽  
Hordeum Vulgare L ◽  
Mitotic Abnormalities ◽  
Negative Effect

AbstractThe effects of exogenous polyamines (PAs): spermine (Spm), spermidine (Spd), cadaverine (Cad) and putrescine (Put) on mitotic activity and chromosomal aberrations in root meristem cells of Hordeum vulgare L. (barley) seeds exposed to salinity were analyzed. The PAs significantly inhibited cell division in distilled water. Furthermore, most of these PAs (except for Spd) caused a significant increase in the frequency of chromosomal aberrations as compared to control group. Seeds treated with Put caused the highest percentage of mitotic abnormalities in total. The negative effect of salinity on mitotic index and the frequency of chromosomal aberrations increased with increasing salt concentration. PAs studied could not be successful in ameliorating of the negative effect of salinity on mitotic activity. Particularly, exposure to Cad and 0.40 M NaCl caused a complete block of cell division in total. However, most of the PA studied showed a perfectly performance in alleviating the detrimental effects of increasing salinity on chromosomal aberrations.

Preparation of pea (Pisum sativum L.) chromosome and nucleus suspensions from single root tips

1996 ◽  
Vol 92 (6) ◽  
pp. 744-751 ◽  
Author(s):  
G. Gualberti ◽  
J. Doležel ◽  
J. Macas ◽  
S. Lucretti
Keyword(s):  
Pisum Sativum ◽  
Root Tips ◽  
Single Root ◽  
Pisum Sativum L

Ecofriendly Synthesis of Silver Nanoparticles and Their Effects on Early Growth and Cell Division in Roots of Green Pea (Pisum sativum L.)

2019 ◽  
Vol 72 (2) ◽  
pp. 113-127
Author(s):  
May Labeeb ◽  
Abdelfattah Badr ◽  
Soliman A. Haroun ◽  
Magdy Z. Mattar ◽  
Aziza S. El-Kholy ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Cell Division ◽  
Pisum Sativum ◽  
Early Growth ◽  
Pisum Sativum L ◽  
Green Pea

Preparation of pea (Pisum sativum? L.) chromosome and nucleus suspensions from single root tips

1996 ◽  
Vol 92 (6) ◽  
pp. 744-751 ◽  
Author(s):  
G. Gualberti ◽  
J. Dolezel ◽  
J. Macas ◽  
J. Macas ◽  
S. Lucretti ◽  
...  
Keyword(s):  
Pisum Sativum ◽  
Root Tips ◽  
Single Root ◽  
Pisum Sativum L

Evaluating cadmium toxicity in the root meristem of Pisum sativum L.

2009 ◽  
Vol 31 (3) ◽  
pp. 531-536 ◽  
Author(s):  
Sazada Siddiqui ◽  
Mukesh K. Meghvansi ◽  
Mushtaq A. Wani ◽  
Farah Jabee
Keyword(s):  
Pisum Sativum ◽  
Root Meristem ◽  
Cadmium Toxicity ◽  
Pisum Sativum L

scholarly journals Evaluation of Cytotoxic and Genotoxic Effect of Acacia nilotica (L.) Delile Extract Using Allium cepa Bioassay

2020 ◽  
pp. 154-159
Author(s):  
Samah Bodowara ◽  
Fauzia El Garaboli ◽  
Salem El shatshat
Keyword(s):  
Cell Division ◽  
Allium Cepa ◽  
Chromosomal Aberrations ◽  
Genetic Material ◽  
Genotoxic Effect ◽  
Bark Extract ◽  
Root Tips ◽  
Mitotic Abnormalities ◽  
Dividing Cells ◽  
Allium Cepa Assay

The present study aimed to measure the cytotoxic and genotoxic effect of the bark of A. nilotica extract. Allium cepa assay was used to find out the effect of A. nilotica extract on chromosome structure and behavior during cell division. The root tips meristem cells were treated with different concentration of A. nilotica bark aqueous extract (0.1, 0.01 and 0.001mg/ml) for 4, 6, 12 and 24 hours, respectively. Cytological analysis revealed decreasing in cell division in all used concentration especially at high ones. The obtained results indicate that aqueous extracts of A. nilotica plant have the ability to decrease the (MI%) values with increasing the concentration at (P<0.005). All treatments have caused different kind of mitotic abnormalities and chromosomal aberrations, such as: change percentage of mitotic phases, C-mitosis, stickiness, chromosome bridges, Micronucleus and vagrant chromosome. The action of A. nilotica bark extract on the genetic material led to decrease in dividing cells number which was concentration and time depended. This inhibition of cell division was due to disturbances in nucleus as a result of inhibition of DNA synthesis. KEY WORDS: Allium cepa assay: A. nilotica: Chromosomal aberrations; MI.

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