CHARACTERIZING VOLATILE FATTY ACIDS AND OTHER GASES IN A RUMEN CLOSED IN VITRO FERMENTATION SYSTEM USING SOLID PHASE MICROEXTRACTION

2003 ◽  
Vol 46 (2) ◽  
Author(s):  
J. P. Spinhirne ◽  
J. A. Koziel ◽  
N. K. Chirase
1974 ◽  
Vol 32 (2) ◽  
pp. 341-351 ◽  
Author(s):  
R. A. Weller ◽  
A. F. Pilgrim

1. A procedure for sampling digesta from within the omasal canal of sheep given a variety of roughage diets was used to enable comparison to be made of the composition of effluent from the reticulo-rumen with that of rumen fluid.2. Concentrations of protozoa in effluents, relative to a soluble marker continuously infused intraruminally, were usually less than 20% of corresponding rumen fluid concentrations. It was estimated that the amount of protozoal nitrogen leaving the rumen represented less than 2% of dietary N.3. Passage of volatile fatty acids (VFA) from the rumen in effluent was less than 75% of that indicated by rumen concentrations.4. A continuous, in vitro fermentation system was developed, in which outputs of protozoa were comparable with in vivo outputs.


1975 ◽  
Vol 46 (11) ◽  
pp. 614-620
Author(s):  
Tsutomu YOSHIDA ◽  
Keiko NAKATANI ◽  
Makoto KANDATSU

1968 ◽  
Vol 19 (5) ◽  
pp. 791 ◽  
Author(s):  
GJ Faichney

Experiments are reported in which sheep were given roughage diets or a high concentrate diet and the VFA absorbed from the rumen were estimated by an in vitro fermentation procedure. The VFA absorbed were compared with the digestible and metabolizable energy intakes of the sheep, determined in digestibility trials, for each diet. For a lucerne diet, a straw diet, and the high concentrate diet the proportions of the digested energy absorbed as VFA were 33.6, 42.4, and 33.2% respectively. On the lucerne diet, the difference between the mean molar proportions of the VFA absorbed and the mean molar proportions of the VFA in the rumen approached significance for acetic acid (P < 0.10) and was highly significant for butyric acid (P < 0.01). The differences were not significant for the other diets.


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