scholarly journals Reference Range Determination for Whole-Blood Platelet Aggregation Using the Multiplate Analyzer

2014 ◽  
Vol 142 (5) ◽  
pp. 647-656 ◽  
Author(s):  
Ellinor I. B. Peerschke ◽  
Donna D. Castellone ◽  
A. K. Stroobants ◽  
John Francis
Keyword(s):  
Platelet Aggregation ◽  
Whole Blood ◽  
Reference Range ◽  
Blood Platelet ◽  
Range Determination ◽  
Multiplate Analyzer ◽  
Blood Platelet Aggregation

scholarly journals Whole Blood Platelet Aggregation and Release Reaction Testing in Uremic Patients

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Jay Zeck ◽  
Jason Schallheim ◽  
Susie Q. Lew ◽  
Louis DePalma
Keyword(s):  
Arachidonic Acid ◽  
Platelet Aggregation ◽  
Whole Blood ◽  
Low Dose ◽  
Reference Range ◽  
Atp Release ◽  
Blood Platelet ◽  
Ckd Stage ◽  
Uremic Patients ◽  
Blood Platelet Aggregation

Background. Platelet function analysis utilizing platelet-rich plasma and optical density based aggregometry fails to identify patients at risk for uremia associated complications.Methods. We employed whole blood platelet aggregation analysis based on impedance as well as determination of ATP release from platelet granules detected by a chemiluminescence method. Ten chronic kidney disease (CKD) stage 4 or 5 predialysis patients underwent platelet evaluation. Our study aims to evaluate this platform in this patient population to determine if abnormalities could be detected.Results. Analysis revealed normal aggregation and ATP release to collagen, ADP, and high-dose ristocetin. ATP release had a low response to arachidonic acid (0.37 ± 0.26 nmoles, reference range: 0.6–1.4 nmoles). Platelet aggregation to low-dose ristocetin revealed an exaggerated response (20.9 ± 18.7 ohms, reference range: 0–5 ohms).Conclusions. Whole blood platelet analysis detected platelet dysfunction which may be associated with bleeding and thrombotic risks in uremia. Diminished ATP release to arachidonic acid (an aspirin-like defect) in uremic patients may result in platelet associated bleeding. An increased aggregation response to low-dose ristocetin (a type IIb von Willebrand disease-like defect) is associated with thrombus formation. This platelet hyperreactivity may be associated with a thrombotic diathesis as seen in some uremic patients.

Multiple electrode aggregometry: A new device to measure platelet aggregation in whole blood

2006 ◽  
Vol 96 (12) ◽  
pp. 781-788 ◽  
Author(s):  
Andreas Calatzis ◽  
Sandra Penz ◽  
Hajna Losonczy ◽  
Wolfgang Siess ◽  
Orsolya Tóth
Keyword(s):  
Platelet Aggregation ◽  
Whole Blood ◽  
Blood Platelet ◽  
Platelet Inhibition ◽  
New Device ◽  
Platelet Aggregometry ◽  
Spontaneous Platelet Aggregation ◽  
Induced Aggregation ◽  
Blood Platelet Aggregation ◽  
Multiple Electrode

SummarySeveral methods are used to analyse platelet function in whole blood. A new device to measure whole blood platelet aggregation has been developed, called multiple electrode platelet aggregometry (MEA). Our aim was to evaluate MEA in comparison with the single platelet counting (SPC) method for the measurement of platelet aggregation and platelet inhibition by aspirin or apyrase in diluted whole blood. Platelet aggregation induced by different concentrations of ADP, collagen and TRAP-6 and platelet inhibition by apyrase or aspirin were determined in citrateor hirudin-anticoagulated blood by MEA and SPC. MEA indicated that spontaneous platelet aggregation was lower, and stimulated platelet aggregation was higher in hirudin- than citrate-anticoagulated blood. In hirudin-anticoagulated, but not citrate-anticoagulated blood, spontaneous platelet aggregation measured by MEA was inhibited by apyrase. For MEA compared with SPC the dose response-curves of agonist-induced platelet aggregation in citrate- and hirudin-blood showed similar EC50 values for TRAP, and higher EC50 values for ADP (non-significant) and collagen (p<0.05). MEA and the SPC method gave similar results concerning platelet-inhibition by apyrase and aspirin. MEA was more sensitive than SPC to the inhibitory effect of aspirin in collagen-induced aggregation. In conclusion, MEA is an easy, reproducible and sensitive method for measuring spontaneous and stimulated platelet aggregation, and evaluating antiplatelet drugs in diluted whole blood. The use of hirudin as an anticoagulant is preferable to the use of citrate. MEA is a promising technique for experimental and clinical applications.

Effect of platelet turnover on whole blood platelet aggregation in patients with coronary artery disease: reply to a rebuttal

2011 ◽  
Vol 9 (4) ◽  
pp. 889-890 ◽  
Author(s):  
E. L. GROVE ◽  
A.-M. HVAS ◽  
S. B. LARSEN ◽  
S. B. MORTENSEN ◽  
S. D. KRISTENSEN
Keyword(s):  
Coronary Artery Disease ◽  
Coronary Artery ◽  
Platelet Aggregation ◽  
Whole Blood ◽  
Blood Platelet ◽  
Artery Disease ◽  
Blood Platelet Aggregation

Comprehensive re-evaluation of historical von Willebrand disease diagnosis in association with whole blood platelet aggregation and function

2018 ◽  
Vol 40 (3) ◽  
pp. 304-311 ◽  
Author(s):  
V. Nummi ◽  
R. Lassila ◽  
L. Joutsi-Korhonen ◽  
E. Armstrong ◽  
T. Szanto
Keyword(s):  
Platelet Aggregation ◽  
Whole Blood ◽  
Blood Platelet ◽  
Disease Diagnosis ◽  
Von Willebrand Disease ◽  
And Function ◽  
Von Willebrand ◽  
Blood Platelet Aggregation

scholarly journals Erythropoietin does not increase whole-blood platelet aggregation in vitro

1994 ◽  
Vol 9 (5) ◽  
pp. 556-558
Author(s):  
J. E. Taylor ◽  
J. J. F. Belch ◽  
I. S. Henderson ◽  
W. K. Stewart
Keyword(s):  
Platelet Aggregation ◽  
Whole Blood ◽  
Blood Platelet ◽  
Blood Platelet Aggregation

Effect of Iron Therapy on the Whole Blood Platelet Aggregation in Infants with Iron Deficiency Anemia*

2000 ◽  
Vol 97 (5) ◽  
pp. 281-285 ◽  
Author(s):  
Ahmet Emin Kürekçi ◽  
A.Avni Atay ◽  
S.Ümit Sarı́cı́ ◽  
Cengiz Zeybek ◽  
Vedat Köseoğlu ◽  
...  
Keyword(s):  
Platelet Aggregation ◽  
Iron Deficiency ◽  
Iron Deficiency Anemia ◽  
Whole Blood ◽  
Blood Platelet ◽  
Iron Therapy ◽  
Deficiency Anemia ◽  
Blood Platelet Aggregation

MEASURING WHOLE BLOOD PLATELET AGGREGATION AND ATP-RELEASE WITH A CHRONO-LOG WHOLE BLOOD LUMI-AGGREGOMETER: EFFECT OF STORAGE TIME OF BLOOD SAMPLES

1987 ◽  
Author(s):  
F C Sieders ◽  
A C v Houwelingen ◽  
G Hornstra
Keyword(s):  
Platelet Aggregation ◽  
Whole Blood ◽  
Storage Time ◽  
Bleeding Time ◽  
Atp Release ◽  
Blood Platelet ◽  
Blood Samples ◽  
Before And After ◽  
Positive Correlations ◽  
Blood Platelet Aggregation

The influence of storing blood for either one or two hours after blood sampling, on whole blood platelet aggregation and ATP-release was measured with a Chrono-log whole blood lumi-aggregometer, in 21 healthy male volunteers. Storage of blood samples, gently revolving at 37 °C in an incubator for one hour, caused a significant increase in aggregation and release as compared with results obtained immediately after sampling. After two hours' storage, the values had returned to their initial levels.Significant positive correlations were seen between values obtained before and after storage of blood, and between various aggregation and release parameters. In this study, bleeding time nor hematocrit values were significantly correlated with the aggregation and release parameters. The considerable influence of storage time on whole blood platelet aggregation and ATP-release underlines the importance of performing these determinations immediately after sampling, or possibly after a standardized storage time. Otherwise, comparison of results -obtained either in clinical situations or in trials - will increase variability as a result of which false conclusions may be obtained. This will be illustrated in a small trial using paracetamol.

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