scholarly journals Transcription Independent Stimulation of Telomerase Enzymatic Activity by HTLV-I Tax Through Stimulation of IKK

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Christophe Nicot
Keyword(s):  
Enzymatic Activity ◽  
Stimulation Of

Isolation of Antithrombin III from Normal and α1-Antitrypsin-Deficient Human Plasma

1977 ◽  
Vol 38 (02) ◽  
pp. 0475-0485 ◽  
Author(s):  
Anna D. Borsodi ◽  
Ralph A. Bradshaw
Keyword(s):  
Enzymatic Activity ◽  
Isoelectric Focusing ◽  
Antithrombin Iii ◽  
Factor Xa ◽  
Antithrombin Activity ◽  
Bovine Trypsin ◽  
Normal Plasma ◽  
Antitrypsin Deficiency ◽  
Simplified Procedure ◽  
Stimulation Of

SummaryThe plasma of individuals, hetero- or homozygous for α1-antitrypsin deficiency, contains greatly decreased amounts of antithrombin activity as assayed against factor Xa. However, heparin stimulation of the residual antithrombin activity is observed, which is comparable to that of normal plasma. Antithrombins isolated from both normal and α1-antitrypsin deficient plasma by a simplified procedure are indistinguishable in both properties and yields. The microheterogeneity observed on isoelectric focusing of both preparations can be eliminated by treatment with neuraminidase. Neither purified human antithrombin nor α1-antitrypsin, when assayed against bovine trypsin, is stimulated by heparin. These results clearly establish the unique natures of antithrombin and α1-antitrypsin and show that about 75% of the antithrombin activity measured in normal plasma is due to α1-antitrypsin. Estimates of anti thrombin III activity in normal plasma by assays dependent on enzymatic activity can probably be obtained only in the presence of heparin.

scholarly journals Effect of aluminium oxide nanoparticles on the enzymatic activity on microorganisms of activated sludge

2018 ◽  
Vol 44 ◽  
pp. 00033 ◽  
Author(s):  
Nina Doskocz ◽  
Katarzyna Affek ◽  
Monika Załęska-Radziwiłł
Keyword(s):  
Enzymatic Activity ◽  
Activated Sludge ◽  
Hydrolytic Enzymes ◽  
Aluminium Oxide ◽  
Cellular Respiration ◽  
Oxide Nanoparticles ◽  
Oxide Compound ◽  
Commercial Use ◽  
Aluminium Oxide Nanoparticles ◽  
Stimulation Of

The increased production and commercial use of nanoparticles (NPs), combined with a lack of regulation regarding their disposal, may result in the unwanted introduction of NPs to wastewater. Wastewater nutrient removal depends on the metabolisms of activated sludge bacteria and their related key enzymes. Therefore, the aim of this work was to determine the effect of aluminium oxide nanoparticles concentrations on the activated sludge enzymatic activity of microorganisms. Tested nanoparticles inhibition cellular respiration in TTC method in the four highest tested concentrations. Moreover, in most samples observed increase dehydrogenase activity. In this study, nano-Al2O3 also caused a clear stimulation of the activity of hydrolytic enzymes microorganisms of activate sludge. Effects of aluminum oxide (compound in bulk forms) on enzymatic activity were different than in the case of the nano from of Al2O3.

Increased Site-Specific Phosphorylation of Tyrosine Hydroxylase Accompanies Stimulation of Enzymatic Activity Induced by Cessation of Dopamine Neuronal Activity

1999 ◽  
Vol 55 (2) ◽  
pp. 202-209 ◽  
Author(s):  
Jow Y. Lew ◽  
Antonio Garcia-Espana ◽  
Kwan Y. Lee ◽  
Kenneth D. Carr ◽  
Menek Goldstein ◽  
...  
Keyword(s):  
Tyrosine Hydroxylase ◽  
Enzymatic Activity ◽  
Neuronal Activity ◽  
Site Specific ◽  
Stimulation Of

COALESCENCE AS A FACTOR IN SOLVENT STIMULATION OF PLASTID PHOSPHATIDASE C ACTIVITY

1957 ◽  
Vol 35 (2) ◽  
pp. 119-126 ◽  
Author(s):  
Morris Kates ◽  
Paul R. Gorham
Keyword(s):  
Enzymatic Activity ◽  
Petroleum Ether ◽  
Ethyl Ether ◽  
Enzymatic Reaction ◽  
Ethyl Butyrate ◽  
Insoluble Complex ◽  
Ether Extraction ◽  
Propyl Ketone ◽  
Stimulation Of

Solvents which stimulate plastid phosphatidase C activity (e.g., ethyl ether, propyl ketone, and ethyl butyrate) have been observed (microscopically) to produce coalescence of lecithin and chloroplast phases, while those which do not stimulate the reaction (e.g., chloroform and petroleum ether) do not produce coalescence. In the ethyl ether-stimulated reaction, all of the original enzymatic activity was associated with the coalesced material, and the lecithin apparently formed an ether-insoluble complex with the chloroplasts; no enzymatic activity appeared in either the aqueous or ether phases. Ether extraction of chloroplasts alone did not liberate the enzyme from the plastids, and the presence of this stimulating solvent was still necessary for reaction with the substrate to occur. It is concluded that stimulating solvents achieve their effect primarily by causing substrate and plastid phases to coalesce and that the enzymatic reaction actually proceeds in the coalesced phase.

Determination of serum aminotransferases: activation by pyridoxal-5'-phosphate in relation to substrate concentration.

1979 ◽  
Vol 25 (1) ◽  
pp. 55-59 ◽  
Author(s):  
J C Hafkenscheid ◽  
C C Dijt
Keyword(s):  
Enzymatic Activity ◽  
Serum Sample ◽  
Aspartate Aminotransferase ◽  
Substrate Concentration ◽  
Enzymatic Reaction ◽  
Reaction Medium ◽  
Aminotransferase Activity ◽  
Serum Aminotransferases ◽  
Stimulation Of

Abstract To investigate the activation of aspartate- and alanine aminotransferases by pyridoxal-5'-phosphate, we determined the enzymatic activity in serum in two different ways: (a) Preincubation of the serum alone or the serum with pyridoxal-5'-phosphate and starting the reaction by the addition of the serum sample or the serum sample + coenzyme, respectively. (b) Preincubation of the serum or the serum with pyridoxal-5'-phosphate in the reaction medium and starting the reaction by adding 2-oxoglutarate. There are only small differences in activities of both aminotransferases determined according to these two different methods. The stimulation by pyridoxal-5'-phosphate is also of the same order, when both methods are compared. Further, these enzymatic activities were measured with use of various concentrations of substrates. From our experiments we conclude that the degree of stimulation of the apoenzyme of the two enzymes is independent of which way the enzymatic reaction is carried out or the substrate concentration, except that aspartate aminotransferase activity is more stimulated by the coenzyme at higher 2-oxoglutarate concentrations.

scholarly journals Enzymatic Activity of the Soybean Ecto-Apyrase GS52 Is Essential for Stimulation of Nodulation

2011 ◽  
Vol 155 (4) ◽  
pp. 1988-1998 ◽  
Author(s):  
Kiwamu Tanaka ◽  
Cuong T. Nguyen ◽  
Marc Libault ◽  
Jianlin Cheng ◽  
Gary Stacey
Keyword(s):  
Enzymatic Activity ◽  
Stimulation Of

scholarly journals THE EFFECT OF NITROGEN ON THE MICROBIOLOGICAL AND BIOCHEMICAL PROPERTIES OF ZINC-CONTAMINATED SOIL

2017 ◽  
Vol 25 (1) ◽  
pp. 13-22 ◽  
Author(s):  
Rafał STRACHEL ◽  
Jadwiga WYSZKOWSKA ◽  
Małgorzata BAĆMAGA
Keyword(s):  
Enzymatic Activity ◽  
Biochemical Properties ◽  
Soil Samples ◽  
Nitrogen Fertilisation ◽  
Water Capacity ◽  
Organotrophic Bacteria ◽  
Maximum Water ◽  
Copiotrophic Bacteria ◽  
Stimulation Of ◽  
Properties Of Soil

The aim of these studies was to determine the influence of excessive zinc doses on the microbiological and enzymatic properties of soil. Also, an evaluation of the possibility to stimulate remediation processes by nitrogen fertilisation of the soil was attempted. Zinc was applied to loamy sand in the amounts of 0, 250, 500, 750, 1000, 1250 mg Zn2+ kg–1 DM soil, while nitrogen in the form of urea in doses of 0, 250, 500 mg N kg–1 DM soil. Soil samples were incubated at a temperature of 25 °C, maintaining a constant humidity equal to 50% of the maximum water capacity. In the 2nd and 20th week of the experiment, the following factors were determined: activity of dehydrogenases and catalase, and number of organotrophic bacteria, copiotrophic bacteria, oligotrophic bacteria, actinomycetes, and fungi. Zinc inhibited the enzymatic activity of the soil, while causing a slight increase in populations of microorganisms. Only fungi reacted unequivocally positively to contamination of the soil with zinc, therefore demonstrating changes in the biodiversity of microorganisms. Nitrogen fertilisation of the soil resulted in stabilization of the environment contaminated with zinc by stimulation of growth of microorganisms resistant to the influence of this metal.

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