scholarly journals THE EFFECT OF NITROGEN ON THE MICROBIOLOGICAL AND BIOCHEMICAL PROPERTIES OF ZINC-CONTAMINATED SOIL

2017 ◽  
Vol 25 (1) ◽  
pp. 13-22 ◽  
Author(s):  
Rafał STRACHEL ◽  
Jadwiga WYSZKOWSKA ◽  
Małgorzata BAĆMAGA
Keyword(s):  
Enzymatic Activity ◽  
Biochemical Properties ◽  
Soil Samples ◽  
Nitrogen Fertilisation ◽  
Water Capacity ◽  
Organotrophic Bacteria ◽  
Maximum Water ◽  
Copiotrophic Bacteria ◽  
Stimulation Of ◽  
Properties Of Soil

The aim of these studies was to determine the influence of excessive zinc doses on the microbiological and enzymatic properties of soil. Also, an evaluation of the possibility to stimulate remediation processes by nitrogen fertilisation of the soil was attempted. Zinc was applied to loamy sand in the amounts of 0, 250, 500, 750, 1000, 1250 mg Zn2+ kg–1 DM soil, while nitrogen in the form of urea in doses of 0, 250, 500 mg N kg–1 DM soil. Soil samples were incubated at a temperature of 25 °C, maintaining a constant humidity equal to 50% of the maximum water capacity. In the 2nd and 20th week of the experiment, the following factors were determined: activity of dehydrogenases and catalase, and number of organotrophic bacteria, copiotrophic bacteria, oligotrophic bacteria, actinomycetes, and fungi. Zinc inhibited the enzymatic activity of the soil, while causing a slight increase in populations of microorganisms. Only fungi reacted unequivocally positively to contamination of the soil with zinc, therefore demonstrating changes in the biodiversity of microorganisms. Nitrogen fertilisation of the soil resulted in stabilization of the environment contaminated with zinc by stimulation of growth of microorganisms resistant to the influence of this metal.

scholarly journals Microbiological and Biochemical Properties in Eutric/Dystric Brunic Arenosols, Eutric/Endocalcaric Cambisols, and Haplic/Albic Luvisols Soils

2021 ◽  
Author(s):  
Małgorzata Baćmaga ◽  
Jadwiga Wyszkowska ◽  
Agata Borowik ◽  
Jan Kucharski ◽  
Łukasz Paprocki
Keyword(s):  
Enzymatic Activity ◽  
Bacterial Communities ◽  
Diversity Index ◽  
Biochemical Properties ◽  
Colony Development ◽  
Class Level ◽  
Study Results ◽  
The Family ◽  
Organotrophic Bacteria

AbstractTo understand the role of microorganisms in the functioning of forest ecosystems, the structure of bacterial communities and the enzymatic activity were determined in forest soils representing the following soil subtypes: Eutric/Dystric Brunic Arenosols (A), Eutric/Endocalcaric Cambisols (C), and Haplic/Albic Luvisols (L). Their microbiological and biochemical properties were compared based on bacterial counts and diversity, and activities of seven soil enzymes: dehydrogenases, catalase, urease, acid phosphatase, alkaline phosphatase, arylsulfatase, and β-glucosidase. Organotrophic bacteria and actinobacteria were the most abundant and featured the highest values of the EP (ecophysiological diversity index) in the Haplic/Albic Luvisol soil. In turn, the CD (colony development index) values of these bacterial groups were the highest in the Eutric/Endocalcaric Cambisols. The OTU number of bacteria allowed concluding that, at the class level, the Eutric/Dystric Brunic Arenosols and Haplic/Albic Luvisols were predominated by Alphaproteobacteria belonging to Proteobacteria, whereas the Eutric/Endocalcaric Cambisols by Actinobacteria. At the family rank, the Eutric/Dystric Brunic Arenosols were colonized in the highest numbers by Mycobacteriaceae, Rhodospirillaceae, Koribacteriaceae, and Acidobacteriaceae; the Eutric/Endocalcaric Cambisols by Nocardiaceae, Bradyrhizobiaceae, and Mycobacteriaceae, whereas Haplic/Albic Luvisols by Sinobacteriaceae and Rhodospirillaceae. Four bacterial genera, i.e., Rhodoplanes, Burkholderia belonging to Proteobacteria, Mycobacterium belonging to Actinobacteria, and Candidatus Solibacter belonging to Acidobacteria, were identified in all soils tested. The genetic diversity of bacteria was proved the highest in Eutric/Endocalcaric Cambisols. In turn, the highest enzymatic activity was found for Haplic/Albic Luvisols, while the lowest one for Eutric/Endocalcaric Cambisols. The present study results point out to significant differences between the soil types analyzed in terms of the diversity and structure of their bacterial communities and their enzymatic properties.

scholarly journals Impact of the Tillage System on the Soil Enzymatic Activity

2012 ◽  
Vol 38 (1) ◽  
pp. 75-82 ◽  
Author(s):  
Elżbieta Bielińska ◽  
Agnieszka Mocek-Płóciniak
Keyword(s):  
Enzymatic Activity ◽  
Soil Samples ◽  
Soil Tillage ◽  
No Tillage ◽  
Soil Environment ◽  
Soil Enzymatic Activity ◽  
Wide Range ◽  
Tillage System ◽  
Chemical Conditions ◽  
Stimulation Of

Impact of the Tillage System on the Soil Enzymatic Activity The aim of this study was to determine the effect of the soil tillage system on soil enzymatic activity. The performed investigations, employing two soil tillage systems: classical (ploughing) and simplified (no-tillage), were carried out on Luvisols and Arenosols differing typologically, with regard to their kind and species. The activity of the following five enzymes was determined in soil samples: dehydrogenases, acid phosphatase, alkaline phosphatase, urease and protease. The applied enzymes tests turned out to be good indicators differentiating the examined soil objects depending on the employed tillage system. The employment of the simplified tillage system stimulated significantly the activity of the analysed enzymes irrespective of the soil type. This effect was particularly apparent in the top layer (0-10 cm) of the soil. An exceptionally wide range of activity was obtained for dehydrogenases indicating the usefulness of this group of enzymes for the evaluation of changes in the soil environment under the influence of the soil tillage system. The observed activity stimulation of the examined enzymes was accompanied by advantageous changes in soil chemical conditions.

Isolation of Antithrombin III from Normal and α1-Antitrypsin-Deficient Human Plasma

1977 ◽  
Vol 38 (02) ◽  
pp. 0475-0485 ◽  
Author(s):  
Anna D. Borsodi ◽  
Ralph A. Bradshaw
Keyword(s):  
Enzymatic Activity ◽  
Isoelectric Focusing ◽  
Antithrombin Iii ◽  
Factor Xa ◽  
Antithrombin Activity ◽  
Bovine Trypsin ◽  
Normal Plasma ◽  
Antitrypsin Deficiency ◽  
Simplified Procedure ◽  
Stimulation Of

SummaryThe plasma of individuals, hetero- or homozygous for α1-antitrypsin deficiency, contains greatly decreased amounts of antithrombin activity as assayed against factor Xa. However, heparin stimulation of the residual antithrombin activity is observed, which is comparable to that of normal plasma. Antithrombins isolated from both normal and α1-antitrypsin deficient plasma by a simplified procedure are indistinguishable in both properties and yields. The microheterogeneity observed on isoelectric focusing of both preparations can be eliminated by treatment with neuraminidase. Neither purified human antithrombin nor α1-antitrypsin, when assayed against bovine trypsin, is stimulated by heparin. These results clearly establish the unique natures of antithrombin and α1-antitrypsin and show that about 75% of the antithrombin activity measured in normal plasma is due to α1-antitrypsin. Estimates of anti thrombin III activity in normal plasma by assays dependent on enzymatic activity can probably be obtained only in the presence of heparin.

Soil response to chemicals used in a field experiment

2013 ◽  
Vol 27 (2) ◽  
pp. 151-158 ◽  
Author(s):  
S. Jezierska-Tys ◽  
A. Rutkowska
Keyword(s):  
Alkaline Phosphatase ◽  
Enzymatic Activity ◽  
Field Experiment ◽  
Soil Microorganisms ◽  
Block Design ◽  
Soil Samples ◽  
Alkaline Phosphatases ◽  
Soil Response ◽  
Negative Effect ◽  
Set Up

Abstract The effect of chemicals (Reglone 200 SL and Elastiq 550 EC) on soil microorganisms and their enzymatic activity was estimated. The study was conducted in a field experiment which was set up in the split-block design and comprised three treatments. Soil samples were taken six times, twice in each year of study. The results showed that the application of chemicals generally had no negative effect on the number of soil microorganisms. The application of Reglone 200 SL caused an increase of proteolytic and ureolytic activity and affected the activity of dehydrogenases, acid and alkaline phosphatases in the soil. The soil subjected of Elastiq 550 EC was characterized by lower activity of dehydrogenases, protease, urease and alkaline phosphatase.

scholarly journals Investigation of geotechnical properties of soil samples in Isu-Njaba Imo State

2021 ◽  
Vol 1036 (1) ◽  
pp. 012035
Author(s):  
A C Ekeleme ◽  
O H Ibearugbulem ◽  
E I Ugwu ◽  
C E Njoku ◽  
E C Amanamba ◽  
...  
Keyword(s):  
Soil Samples ◽  
Geotechnical Properties ◽  
Imo State ◽  
Properties Of Soil

scholarly journals STUDY AND ESTIMATION OF THE RATIO OF 137CS AND 40K SPECIFIC ACTIVITIES IN SANDY AND LOAM SOILS / 137CS IR 40K SAVITŲJŲ AKTYVUMŲ SANTYKIŲ TYRIMAS IR VERTINIMAS PRIESMĖLIO IR PRIEMOLIO DIRVOŽEMYJE

2011 ◽  
Vol 3 (5) ◽  
pp. 37-42
Author(s):  
Renata Mikalauskienė ◽  
Donatas Butkus ◽  
Ingrida Pliopaitė Bataitienė
Keyword(s):  
Chemical Properties ◽  
Dry Weight ◽  
Soil Samples ◽  
Physical And Chemical Properties ◽  
Nuclear Testing ◽  
Specific Activities ◽  
Physical And Chemical ◽  
Chernobyl Nuclear Plant ◽  
Properties Of Soil ◽  
And Chemical Properties

The present article describes changes in specific activities and fluctuations in the ratio of natural 40K and artificial 137Cs radionuclides in soil samples taken from different places of Lithuanian territory. The samples of soil have been selected from the districts polluted after the accident in Chernobyl nuclear plant performing nuclear testing operations. The study has established the main physical and chemical properties of soil samples and their impact on the concentration of 40K activities. 137Cs/40K specific activities in soil have been observed under the dry weight of the sample that varied from 0.0034 to 0.0240. The results of the study could be used for establishing and estimating 137Csand 40K transfer in the system “soil-plant”. Santrauka Straipsnyje nagrinėjama gamtinės (40K) ir dirbtinės (137Cs) kilmės radionuklidų savitųjų aktyvumų ir jų santykio kaita skirtinguose Lietuvos teritorijos dirvožemiuose. Dirvožemio mėginiai parinkti iš vietovių, kurios buvo labiau užterštos po Černobylio atominės elektrinės avarijos ir buvusių branduolinių bandymų. Tyrimo metu nustatytos pagrindinės fizinės cheminės dirvožemio savybės ir jų poveikis 40K aktyvumų koncentracijai. 137Csir 40K savitieji aktyvumai dirvožemyje tirti esant sausam mėginio svoriui. 137Cs savitieji aktyvumai sausame dirvožemyje svyravo nuo 1,1±1,0 iki 14,3±0,9 Bq/kg, o 40K savitieji aktyvumai – nuo 326±29 iki 740±15 Bq/kg. 137Csir 40K savitųjų aktyvumų santykis skirtingų vietovių dirvožemiuose kito nuo 0,0034 iki 0,0240 Bq/kg. Tyrimo rezultatai gali būti panaudoti, nustatant ir įvertinant 137Csir 40K pernašą sistemoje dirvožemis–augalai.

scholarly journals Effect of Bentonite and Barley Straw on the Restoration of the Biological Quality of Agriculture Soil Contaminated with the Herbicide Successor T 550 SE

Agriculture ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 27
Author(s):  
Jadwiga Wyszkowska ◽  
Monika Tomkiel ◽  
Agata Borowik ◽  
Małgorzata Baćmaga ◽  
Jan Kucharski
Keyword(s):  
Agricultural Soil ◽  
Plant Protection ◽  
Spring Barley ◽  
Biochemical Properties ◽  
Barley Straw ◽  
Negative Effects ◽  
Soil Quality Index ◽  
Oligotrophic Bacteria ◽  
Organotrophic Bacteria ◽  
Environmentally Safe

Environmentally safe ways are sought to prevent the accumulation and to accelerate the degradation of herbicide active substances in agricultural soil. This study aimed to determine the effectiveness of finely-ground barley straw and bentonite in mitigating the effects of agricultural soil contamination with Successor T 550 SE. This herbicide was applied in the following doses: 0, 0.73, and 14.63 mg of the active substance per kg. The bentonite and spring barley straw were used at 10 g/kg. The action of these additives was compared to soil without the addition of straw and bentonite. The application of the experimental herbicide disturbed microbial systems, such as organotrophic bacteria, oligotrophic bacteria and their spores, actinobacteria, and fungi. A positive response to the herbicide dose of 14.63 mg a.s./kg was observed only for spores of oligotrophic bacteria. Further disturbances were observed in the agricultural soil biochemical properties, i.e., in the activity of dehydrogenases, urease, catalase, acid, and alkaline phosphatase, arylsulfatase, and β-glucosidase. A significant decrease in the activity of dehydrogenases, acid phosphatase, and arylsulfatase was observed following the application of 14.63 mg a.s./kg. The yield of maize decreased following the application of the analysed plant protection agent. Based on the soil quality index (BA), the addition of straw was more effective in restoring soil homeostasis than bentonite. Both bentonite and straw can be successfully used to improve agricultural soil biological activity. However, more effective mitigation of the negative effects of the herbicide in soil was observed in objects supplemented with barley straw. This improved the microbiological and biochemical properties of the soil. Barley straw was more effective than bentonite in restoring soil biological balance.

Stabilization of Black Cotton Soil using Lime and Phosphogypsum

2021 ◽  
Vol 9 (4) ◽  
pp. 503-507
Keyword(s):  
Unconfined Compressive Strength ◽  
Expansive Soils ◽  
Soil Samples ◽  
Black Cotton Soil ◽  
Stabilization Method ◽  
Curing Period ◽  
Problematic Soils ◽  
Construction Purpose ◽  
Black Cotton Soils ◽  
Properties Of Soil

Expansive soils are problematic soils for Civil Engineers. Black cotton (BC) soils possess low strength and high compressibility, due to these properties black cotton soils are considered to be challenging one for analysis. To achieve desired properties of soil for construction purpose these black cotton soil must be enhanced to meet its requirement. To modify the properties of black cotton soils, many treatment methods are there. In this paper an attempt has been made to improve the properties of black cotton soil by using industrial waste through stabilization method. By stabilizing the soil properties are enhanced and make it suitable for subgrade construction. In this work, the combined effect of Lime and Phosphogypsum (PG) on compaction characteristics, Atterberg’s Limit, Unconfined Compressive Strength (UCS) for original soil, California Bearing Ratio (CBR) and direct shear Test of a black cotton soil with percentage varying of Lime and Phosphogypsum was carried out. The soil samples were tested for tri-axial compression test and CBR tests were carried out after 4 days curing period. From the results, it has been inferred that the black cotton soil treated with Lime and Phosphogypsum in the percentages of (4:4) has better strength characteristics. Hence, it may be concluded that Lime and Phosphogypsum can be used for stabilization of black cotton soils for pavement subgrade

scholarly journals Effect of aluminium oxide nanoparticles on the enzymatic activity on microorganisms of activated sludge

2018 ◽  
Vol 44 ◽  
pp. 00033 ◽  
Author(s):  
Nina Doskocz ◽  
Katarzyna Affek ◽  
Monika Załęska-Radziwiłł
Keyword(s):  
Enzymatic Activity ◽  
Activated Sludge ◽  
Hydrolytic Enzymes ◽  
Aluminium Oxide ◽  
Cellular Respiration ◽  
Oxide Nanoparticles ◽  
Oxide Compound ◽  
Commercial Use ◽  
Aluminium Oxide Nanoparticles ◽  
Stimulation Of

The increased production and commercial use of nanoparticles (NPs), combined with a lack of regulation regarding their disposal, may result in the unwanted introduction of NPs to wastewater. Wastewater nutrient removal depends on the metabolisms of activated sludge bacteria and their related key enzymes. Therefore, the aim of this work was to determine the effect of aluminium oxide nanoparticles concentrations on the activated sludge enzymatic activity of microorganisms. Tested nanoparticles inhibition cellular respiration in TTC method in the four highest tested concentrations. Moreover, in most samples observed increase dehydrogenase activity. In this study, nano-Al2O3 also caused a clear stimulation of the activity of hydrolytic enzymes microorganisms of activate sludge. Effects of aluminum oxide (compound in bulk forms) on enzymatic activity were different than in the case of the nano from of Al2O3.

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