Mutagenic Effectiveness and Efficiency of Individual and Combination Treatments of Gamma Rays and Ethyl Methanesulfonate in Black Gram [Vigna mungo (L.) Hepper]

In mutation breeding, mutagenic effectiveness and efficiency are the base parameters to predict the mutagenic potency of any mutagen. Studies on mutagenic effectiveness and mutagenic efficiency of physical mutagen (gamma rays) and chemical mutagen (hydrazine hydrates; HZ) on two cultivars of lentil (Lens culinaris Medik.), viz. DPL 62 (macrosperma) and Pant L 406 (microsperma) have been reported. Dry and healthy seeds were treated with four doses of each gamma rays (100-400 Gy), HZ (0.1-0.4 %) and their combinations. Frequencies of the induced agro-morphological variations into different phenotypic categories were estimated in M2 population that resulted into identification and isolation of wide range of mutants with altered phenotypes. Data on effectiveness and efficiency of various mutagenic treatments calculated on the basis of the frequency of chlorophyll mutations, which showed effectiveness and efficiency were higher at the moderate doses of gamma rays and HZ, while in case of combination treatments; lower doses were most effective and efficient with few inter-varietal exceptions. Phenotyping of the mutants revealed that growth habits was the most sensitive category to which most of the mutant belongs, followed by leaf and flower/pod/seed in both the cultivars studied. Overall, the screened and isolated mutants with economically important agronomic traits can be further propagated in the subsequent generation for development of elite lentil mutant cultivars.

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Comparative Studies on Mutagenic Effectiveness and Efficiency in Horsegram [Macrotyloma uniflorum (Lam) Verdc.]

Legume Research - An International Journal ◽

10.18805/lr-4408 ◽

2020 ◽

Author(s):

S. Priyanka ◽

R. Sudhagar ◽

C. Vanniarajan ◽

K. Ganesamurthy ◽

J. Souframanien ◽

...

Keyword(s):

Gamma Rays ◽

Wide Spectrum ◽

Block Design ◽

Seed Fertility ◽

Combination Treatments ◽

Macrotyloma Uniflorum ◽

Randomized Block Design ◽

Methane Sulphonate ◽

Chlorophyll Mutants ◽

Effectiveness And Efficiency

Background: Two horsegram varieties viz., PAIYUR 2 and CRIDA1-18R were mutated with gamma rays (G), electron beam (EB), G+EB and G+EMS (ethyl methane sulphonate) to determine the mutagenic potency in breeding programme.Methods: Uniform seeds treated with different mutagenic doses were raised in randomized block design which constituted M1 generation. Each plant was harvested individually and forwarded to M2 generation following plant to progeny row method.Result: A dose dependant decline was observed for seed germination, plant survival, root length, shoot length, plant height, pollen fertility and seed fertility in M1 population. Wide spectrum of chlorophyll mutants was induced in M2 generation with maximum frequency at EB followed by combination treatments (G+EMS and G+EB) in both varieties. The mutagenic effectiveness ranged from 0.14 per cent to 1.45 per cent in PAIYUR 2 and 0.15 per cent to 1.71 per cent in CRIDA1-18R. The high mutation rate for effectiveness was exhibited by G and G+EB. With reference to sterility, EB was found to be efficient mutagen in both varieties whereas varied efficiency was noted for lethality (EB - PAIYUR 2; G+EMS - CRIDA1-18R) and injury (G - PAIYUR 2; G+EMS - CRIDA1-18R).

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