Determination of mono- and diacylglycerols in edible oils and fats by high performance liquid chromatography and evaporative light scattering detector: Results of collaborative studies and the standardised method

The development, by collaborative study, of standardised method for the determination of mono- and diacylglycerols in vegetable oils and fats is described. The method involves separation of mono- and diacylglycerols by normal phase high-performance liquid-liquid chromatography (HPLC) and evaporative light scattering detection of a solution of oil, fat or a commercial mono- and diacylglycerol preparation in a organic solvent.