Coherent noise reduction of phase images in digital holographic microscopy based on the adaptive anisotropic diffusion

2018 ◽  
Vol 57 (19) ◽  
pp. 5364 ◽  
Author(s):  
Yumin Wu ◽  
Haobo Cheng ◽  
Yongfu Wen ◽  
Xin Chen ◽  
Yingwei Wang
Keyword(s):  
Noise Reduction ◽  
Anisotropic Diffusion ◽  
Digital Holographic Microscopy ◽  
Coherent Noise ◽  
Phase Images ◽  
Holographic Microscopy

Coherent noise reduction of reconstruction of digital holographic microscopy using a laterally shifting hologram aperture

2016 ◽  
Vol 55 (12) ◽  
pp. 121725 ◽  
Author(s):  
Yun Liu ◽  
Zhao Wang ◽  
Junhui Huang ◽  
Jianmin Gao ◽  
Jiansu Li ◽  
...  
Keyword(s):  
Noise Reduction ◽  
Digital Holographic Microscopy ◽  
Coherent Noise ◽  
Holographic Microscopy

scholarly journals Dynamic Speckle Illumination Digital Holographic Microscopy by Doubly Scattered System

Photonics ◽  
2021 ◽  
Vol 8 (7) ◽  
pp. 276
Author(s):  
Yun Liu ◽  
Peihua Bu ◽  
Mingxing Jiao ◽  
Junhong Xing ◽  
Ke Kou ◽  
...  
Keyword(s):  
Time Correlation ◽  
Digital Holographic Microscopy ◽  
Laser Source ◽  
Speckle Reduction ◽  
Single Shot ◽  
Coherent Noise ◽  
Real Time Measurement ◽  
Dynamic Speckle ◽  
Holographic Microscopy ◽  
Speckle Suppression

The coherent noise always exists in digital holographic microscopy due to the laser source, degrading the image quality. A method of speckle suppression using the dynamic speckle illumination, produced by double-moving diffusers, is presented in digital holographic microscopy. The space–time correlation functions are theoretically analyzed from the statistics distribution in the doubly and singly scattered system, respectively. The configuration of double-moving diffusers is demonstrated to have better performance in speckle suppression compared with the single diffuser and moving-static double diffusers cases. The experiment results verify the feasibility of the approach. The presented approach only requires a single shot interferogram to realize the speckle reduction, accordingly it has the potential application in real-time measurement.

scholarly journals Measuring the Cell-Induced Deformation of Collagen Matrix Detected With Digital Holographic Microscopy

2007 ◽  
Author(s):  
Kaveh Azartash ◽  
Enrico Gratton
Keyword(s):  
Mammalian Cells ◽  
Collagen Matrix ◽  
Spatial Arrangement ◽  
Digital Holographic Microscopy ◽  
Physical Aspect ◽  
Reconstruction Algorithms ◽  
Phase Images ◽  
Holographic Microscopy ◽  
Set Up ◽  
Charged Couple Device

A modified Mach-Zender set-up in reflection is applied to record and reconstruct holographic amplitude and phase images. A charged couple device (CCD) is used to record a hologram and numerical reconstruction algorithms are then applied to rebuild the hologram for obtaining both phase and amplitude information. One could also focus on multiple focal planes from a single hologram, similar to the focusing control of a conventional microscope. The morphology and behavior of mammalian cells is determined by an interaction between signals from the intracellular matrix and the cellular responses. It is important to note that the physical aspect of the extracellular matrix is as significant as the chemical nature of it. Specifically the stresses, mechanical forces, and the profile of the external environment have major effects on cell behavior. The mechanical and physical characteristics of a tissue are greatly dependent on a hierarchical spatial arrangement of its extra-cellular matrix components. A key player in the ECM is collagen which exhibits significant tensile strength on the cellular scale. Digital holographic microscopy (DHM) is applied to study the deformation of collage matrix in response to cell migration.

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