Spatial refractive index distribution and glaucoma-related tissue density alterations of the retina determined by multi-spectral digital holographic microscopy

2020 ◽  
Author(s):  
Björn Kemper ◽  
Alvaro Barroso ◽  
Steffi Ketelhut ◽  
Peter Heiduschka ◽  
Jürgen Schnekenburger ◽  
...  
Keyword(s):  
Refractive Index ◽  
Digital Holographic Microscopy ◽  
Refractive Index Distribution ◽  
Tissue Density ◽  
Index Distribution ◽  
Holographic Microscopy

Digital holographic microscopy of highly sensitive living cells

2021 ◽  
pp. 1-13
Author(s):  
Shuntao Yang
Keyword(s):  
Living Cell ◽  
Phase Distribution ◽  
High Sensitivity ◽  
Living Cells ◽  
Digital Holographic Microscopy ◽  
Refractive Index Distribution ◽  
Highly Sensitive ◽  
Index Distribution ◽  
Holographic Microscopy ◽  
Cell Microscopy

In order to solve the problem that the existing living cell microscopy technology can not display the detailed information of cells, a high sensitivity digital holographic living cell microscopy technology is proposed in this paper. By measuring the phase distribution and refractive index distribution of living cells, the data of living cells are extracted and converted into digital hologram of living cells. Simulation and comparison of the commonly used two-dimensional living cell microscope methods. The experimental results show that the high-sensitivity digital holographic microscopic detection method can obtain the detailed information of living cells, which proves the effectiveness of this study.

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