scholarly journals Accuracy of the Gaussian Point Spread Function model in 2D localization microscopy

2010 ◽  
Vol 18 (24) ◽  
pp. 24461 ◽  
Author(s):  
Sjoerd Stallinga ◽  
Bernd Rieger
2020 ◽  
Vol 40 (14) ◽  
pp. 1405003
Author(s):  
卢泉 Lu Quan ◽  
张泽昊 Zhang Zehao ◽  
张卫平 Zhang Weiping ◽  
刘诣荣 Liu Yirong

2019 ◽  
Vol 27 (5) ◽  
pp. 6147 ◽  
Author(s):  
Eran Hershko ◽  
Lucien E. Weiss ◽  
Tomer Michaeli ◽  
Yoav Shechtman

2018 ◽  
Author(s):  
Rasmus Ø. Thorsen ◽  
Christiaan N. Hulleman ◽  
Mathias Hammer ◽  
David Grünwald ◽  
Sjoerd Stallinga ◽  
...  

Recently, Franke, Sauer and van de Linde1 introduced a way to estimate the axial position of single-molecules (TRABI). To this end, they compared the detected photon count from a temporal radial-aperture-based intensity estimation to the estimated count from Gaussian point-spread function (PSF) fitting to the data. Empirically they found this photometric ratio to be around 0.7-0.8 close to focus and decreasing away from it. Here, we explain this reported but unexplained discrepancy and furthermore show that the photometric ratio as indicator for axial position is susceptible even to typical optical aberrations.


2020 ◽  
Vol 159 (4) ◽  
pp. 183 ◽  
Author(s):  
Peng Jia ◽  
Xuebo Wu ◽  
Huang Yi ◽  
Bojun Cai ◽  
Dongmei Cai

Sign in / Sign up

Export Citation Format

Share Document