Generation of Hybrid Optical Trap Array by Holographic Optical Tweezers

Enabled by multiple optical traps, holographic optical tweezers can manipulate multiple particles in parallel flexibly. Spatial light modulators are widely used in holographic optical tweezers, in which Gaussian point (GP) trap arrays or special mode optical trap arrays including optical vortex (OV) arrays, perfect vortex (PV) arrays, and Airy beam arrays, etc., can be generated by addressing various phase holograms. However, the optical traps in these arrays are almost all of the same type. Here, we propose a new method for generating a hybrid optical trap array (HOTA), where optical traps such as GPs, OVs, PVs, and Airy beams in the focal plane are combined arbitrarily. Also, the axial position and peak intensity of each them can be adjusted independently. The energy efficiency of this method is theoretically studied, while different micro-manipulations on multiple particles have been realized with the support of HOTA experimentally. The proposed method expands holographic optical tweezers’ capabilities and provides a new possibility of multi-functional optical micro-manipulation.

Finite Element Thermal Analysis of Melt Pool in Selective Laser Melting Process

Selective laser melting is one of the powder bed fusion processes which fabricates a part through layer-wised method. Due to the ability to build a customized and complex part, selective laser melting process has been broadly studied in academic and applied in industry. However, rapidly changed thermal cycles and extremely high-temperature gradients among the melt pool induce a periodically changed thermal stress in solidified layers and finally result in a distorted part. Therefore, the temperature distribution in the melt pool and the size and shape of the melt pool directly determine the mechanical and geometrical property of final part. As experimental trial-and-error method takes a huge amount of cost, different numerical methods have been adopted to estimate the transient temperature and thermal stress distribution in the melt pool and powder bed. The most existing research utilizes the moving Gaussian point heat source to model the profile of the melt pool, which consumes a significant amount of computational cost and cannot be used to implement the part-level simulation. This research proposes a new line heat source to replace the moving point heat source. Some efforts are applied to reduce the computational cost. Specifically, a relatively large step size is used for the line heat source to reduce the number of time steps. In addition, a mesh refinement scheme is adopted to reduce the number of cells in each time step by refining the mesh close to the heat source and coarsening the mesh far away from it. On the other hand, efforts are implemented to increase the accuracy of the simulation result. Temperature-dependent material properties are considered in this FE framework. In addition, material transition among powder, liquid, and solid are incorporated in the developed FE framework. In this study, temperature simulation of one scanning track based on self-developed FE code is applied for Stainless Steel 316L. The simulation results show that the temperature distribution and history of melt pool within line heat source are comparable to that of the moving Gaussian point heat source. While the simulation time is reduced by more than two times depending on the length of line heat input. Therefore, this FE model can be used to numerically investigate the process parameters and help to control the quality of the final part.

Photon count estimation in single-molecule localization microscopy

Recently, Franke, Sauer and van de Linde1 introduced a way to estimate the axial position of single-molecules (TRABI). To this end, they compared the detected photon count from a temporal radial-aperture-based intensity estimation to the estimated count from Gaussian point-spread function (PSF) fitting to the data. Empirically they found this photometric ratio to be around 0.7-0.8 close to focus and decreasing away from it. Here, we explain this reported but unexplained discrepancy and furthermore show that the photometric ratio as indicator for axial position is susceptible even to typical optical aberrations.