A Resonance Raman Method for the Rapid Detection and Identification of Bacteria in Water

1980 ◽  
Vol 34 (1) ◽  
pp. 72-75 ◽  
Author(s):  
W. F. Howard ◽  
W. H. Nelson ◽  
J. F. Sperry
Keyword(s):  
Raman Spectra ◽  
Rapid Detection ◽  
Resonance Raman ◽  
Aqueous Dispersion ◽  
Incident Radiation ◽  
Detection And Identification ◽  
Identification Of Bacteria ◽  
Resonance Raman Spectra ◽  
Combination Bands

Resonance Raman spectra are reported for 16 types of carotene-containing bacteria and algae in aqueous dispersion. Spectra are obtained with ease from organisms grown in culture and collected by centrifugation. In many instances spectra produced with 488 nm incident radiation are sufficiently unique to provide a basis for identification. While most information is contained in the 900 to 1600 cm−1 region, several bacteria exhibit pronounced carotenoid overtone and combination bands which can be assigned along with the fundamental vibrations.

scholarly journals Resonance Raman Spectra for the In Situ Identification of Bacteria Strains and Their Inactivation Mechanism

2021 ◽  
pp. 000370282199283
Author(s):  
Dinesh Dhankhar ◽  
Anushka Nagpal ◽  
Runze Li ◽  
Jie Chen ◽  
Thomas C. Cesario ◽  
...  
Keyword(s):  
Radiation Dose ◽  
Raman Spectra ◽  
Resonance Raman ◽  
Bacterial Strains ◽  
Short Period ◽  
Intensity Changes ◽  
Inactivation Mechanism ◽  
Identification Of Bacteria ◽  
Resonance Raman Spectra

The resonance Raman spectra of bacterial carotenoids have been employed to identify bacterial strains and their intensity changes as a function of ultraviolet (UV) radiation dose have been used to differentiate between live and dead bacteria. In addition, the resonance-enhanced Raman spectra enabled us to detect bacteria in water at much lower concentrations (∼108 cells/mL) than normally detected spectroscopically. A handheld spectrometer capable of recording resonance Raman spectra in situ was designed, constructed, and was used to record the spectra. In addition to bacteria, the method presented in this paper may also be used to identify fungi, viruses, and plants, in situ, and detect infections within a very short period of time.

scholarly journals Resonance Raman spectra for the in-situ identification of bacteria strains and their inactivation mechanism

2020 ◽  
Author(s):  
Dinesh Dhankhar ◽  
Anushka Nagpal ◽  
Runze Li ◽  
Jie Chen ◽  
Thomas C. Cesario ◽  
...  
Keyword(s):  
Raman Spectra ◽  
Resonance Raman ◽  
Bacterial Strains ◽  
Short Period ◽  
Intensity Changes ◽  
Inactivation Mechanism ◽  
Identification Of Bacteria ◽  
Resonance Raman Spectra ◽  
Pigmented Bacteria

AbstractThe resonance Raman spectra of bacterial carotenoids have been employed to identify bacterial strains and their intensity changes as a function of ultraviolet(UV) radiation dose have been used to differentiate between live and dead bacteria. The enhanced resonance Raman spectra of color-pigmented bacteria were recorded after excitation with visible light diode lasers. In addition, the resonance enhanced Raman spectra enabled us to detect bacteria in water at much lower concentrations (~108 cells/mL) than normally detected spectroscopically. A handheld spectrometer capable of recording resonance Raman spectra in-situ was designed, constructed and was used to record the spectra. In addition to bacteria, the method presented in this paper may also be used to identify fungi, viruses and plants, in-situ, and detect infections within a very short period of time.

A Resonance Raman Method for the Rapid Detection and Identification of Algae in Water

1983 ◽  
Vol 37 (1) ◽  
pp. 55-58 ◽  
Author(s):  
S. K. Brahma ◽  
P. E. Hargraves ◽  
W. F. Howard ◽  
W. H. Nelson
Keyword(s):  
Resonance Raman ◽  
Carotenoid Pigments ◽  
Entire Region ◽  
Sensing Applications ◽  
Detection And Identification ◽  
Class Level ◽  
Rapid Flow ◽  
Remote Sensing Applications ◽  
Resonance Raman Spectra ◽  
Combination Bands

Resonance Raman spectra are reported for aqueous suspensions of nine clones of marine plankton algae representing three classes, five genera, and seven species. Spectra are obtained easily either directly from culture or from samples concentrated by sedimentation. Spectra taken at 488 or 457.9 nm are of high quality and are sufficiently distinct to differentiate clones at the algal class level, and possibly also at the genus level. Strongest peaks occur near 1527 and 1158 cm−1 associated with ν(c = c) and ν(c – c) of carotenoid pigments, but information is contained in the entire region between 900 and 3000 cm−1 due to associated overtone and combination bands which can be assigned along with fundamental vibrations. Chlorophyll peaks also are quite pronounced. Spectra obtained using rapid flow techniques match those taken using slurries in sealed tubes if low laser power is used. The sensitivity and rapidity of the technique suggest that it may be useful in remote sensing applications.

scholarly journals Low-temperature resonance-Raman spectra of Japanese-lacquer-tree (Rhus vernicifera) laccase, type-2-copper-depleted laccase and H2O2-treated type-2-copper-depleted laccase

1983 ◽  
Vol 213 (2) ◽  
pp. 503-506 ◽  
Author(s):  
G Musci ◽  
A Desideri ◽  
L Morpurgo ◽  
A Garnier-Suillerot ◽  
L Tosi
Keyword(s):  
Raman Spectra ◽  
Strong Dependence ◽  
Resonance Raman ◽  
Local Symmetry ◽  
Structural Rearrangements ◽  
Blue Copper ◽  
Copper Site ◽  
Resonance Raman Spectra ◽  
Rhus Vernicifera

Resonance-Raman spectra of Japanese-lacquer-tree (Rhus vernicifera) laccase, type-2-copper-depleted laccase and the latter form treated with H2O2 were measured in liquid and frozen solution, on excitation into the 600 nm absorption band. Significant changes in intensity and/or frequency of the bands lying in the 370-430 cm-1 region were observed on freezing, indicating local structural rearrangements taking place at the blue copper site. These findings corroborate previous suggestions based on e.p.r. measurements and redox data [Morpurgo, Calabrese, Desideri & Rotilio (1981) Biochem. J. 193, 639-642]. They show the strong dependence of the physical properties of blue copper centres on local symmetry. Some conclusions on the origin of the Raman bands are also drawn.

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