Structure of the SCAN Domain of Human Paternally Expressed Gene 3 Protein

Run coding applied to the digitized video signal from a TV scan of cell preparations can effect a substantial reduction in the total amount of data, sufficient to permit a moderate size of store to be loaded within one frame time with a representation of the field adequate for computer analysis. This paper describes the design of a run coding interface between a TV scanner and a computer store which also allows control of scan domain, spatial resolution and density resolution. Results are presented showing its efficiency when dealing with cervical smear preparations.

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Zscan4c activates endogenous retrovirus MERVL and cleavage embryo genes

Nucleic Acids Research ◽

10.1093/nar/gkz594 ◽

2019 ◽

Cited By ~ 4

Author(s):

Weiyu Zhang ◽

Fuquan Chen ◽

Ruiqing Chen ◽

Dan Xie ◽

Jiao Yang ◽

...

Keyword(s):

Developmental Stages ◽

Embryonic Stem ◽

Endogenous Retrovirus ◽

Endogenous Retroviruses ◽

Enhancer Activity ◽

Cell Cleavage ◽

Cell Embryo ◽

And Function ◽

Scan Domain

AbstractEndogenous retroviruses (ERVs) contribute to ∼10 percent of the mouse genome. They are often silenced in differentiated somatic cells but differentially expressed at various embryonic developmental stages. A minority of mouse embryonic stem cells (ESCs), like 2-cell cleavage embryos, highly express ERV MERVL. However, the role of ERVs and mechanism of their activation in these cells are still poorly understood. In this study, we investigated the regulation and function of the stage-specific expressed ERVs, with a particular focus on the totipotency marker MT2/MERVL. We show that the transcription factor Zscan4c functions as an activator of MT2/MERVL and 2-cell/4-cell embryo genes. Zinc finger domains of Zscan4c play an important role in this process. In addition, Zscan4c interacts with MT2 and regulates MT2-nearby 2-cell/4-cell genes through promoting enhancer activity of MT2. Furthermore, MT2 activation is accompanied by enhanced H3K4me1, H3K27ac, and H3K14ac deposition on MT2. Zscan4c also interacts with GBAF chromatin remodelling complex through SCAN domain to further activate MT2 enhancer activity. Taken together, we delineate a previously unrecognized regulatory axis that Zscan4c interacts with and activates MT2/MERVL loci and their nearby genes through epigenetic regulation.

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Structural components of SCAN-domain dimerizations

Proteins Structure Function and Bioinformatics ◽

10.1002/prot.20170 ◽

2004 ◽

Vol 56 (4) ◽

pp. 685-692 ◽

Cited By ~ 13

Author(s):

Kiyean Nam ◽

Christian Honer ◽

Christoph Schumacher

Keyword(s):

Structural Components ◽

Scan Domain

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Heterodimer formation of the myeloid zinc finger 1 SCAN domain and association with promyelocytic leukemia nuclear bodies

Leukemia Research ◽

10.1016/j.leukres.2008.03.024 ◽

2008 ◽

Vol 32 (10) ◽

pp. 1582-1592 ◽

Cited By ~ 14

Author(s):

LeAnne Noll ◽

Francis C. Peterson ◽

Paulette L. Hayes ◽

Brian F. Volkman ◽

Tara Sander

Keyword(s):

Zinc Finger ◽

Promyelocytic Leukemia ◽

Nuclear Bodies ◽

Promyelocytic Leukemia Nuclear Bodies ◽

Myeloid Zinc Finger ◽

Scan Domain

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The Zinc Finger Protein 202 (ZNF202) Is a Transcriptional Repressor of ATP Binding Cassette Transporter A1 (ABCA1) and ABCG1 Gene Expression and a Modulator of Cellular Lipid Efflux

Journal of Biological Chemistry ◽

10.1074/jbc.m100218200 ◽

2001 ◽

Vol 276 (15) ◽

pp. 12427-12433 ◽

Cited By ~ 76

Author(s):

Mustafa Porsch-Özcürümez ◽

Thomas Langmann ◽

Susanne Heimerl ◽

Hana Borsukova ◽

Wolfgang E. Kaminski ◽

...

Keyword(s):

Gene Expression ◽

Zinc Finger ◽

Transcriptional Repressor ◽

Atp Binding ◽

Raw264.7 Macrophages ◽

Atp Binding Cassette Transporter ◽

Lipid Efflux ◽

Functional Linkage ◽

Scan Domain ◽

Atp Binding Cassette

The zinc finger gene 202 (ZNF202) located within a hypoalphalipoproteinemia susceptibility locus on chromosome 11q23 is a transcriptional repressor of various genes involved in lipid metabolism. To provide further evidence for a functional linkage between ZNF202 and hypoalphalipoproteinemia, we investigated the effect of ZNF202 expression on ATP binding cassette transporter A1 (ABCA1) and ABCG1. ABCA1 is a key regulator of the plasma high density lipoprotein pool size, whereas ABCG1 is another mediator of cellular cholesterol and phospholipid efflux in human macrophage. We demonstrate here that the full-length ZNF202m1 isoform binds to GnT repeats within the promotors of ABCA1 (−229/−210) and ABCG1 (−572/−552). ZNF202m1 expression in HepG2 cells dose-dependently repressed the promotor activities of ABCA1 and ABCG1. This transcriptional effect required the presence of the SCAN domain in ZNF202 and the functional integrity of a TATA box at position −24 of ABCA1, whereas the presence of GnT binding motifs was nonessential. The state of ZNF202 SCAN domain oligomerization affected the ability of the adjacent ZNF202 Krüppel-associated box domain to recruit the transcriptional corepressor KAP1. Overexpression of ZNF202m1 in RAW264.7 macrophages prevented the induction of ABCA1 gene expression by 20(S)OH-cholesterol and 9-cis-retinoic acid, further substantiating the interference of ZNF202 in critical elements of transcriptional activation. Finally, HDL and apoAImediated lipid efflux was significantly reduced in RAW264.7 cells stably expressing ZNF202m1. In conclusion, we have identified ABCA1 and ABCG1 as target genes for ZNF202-mediated repression and thus, provide evidence for a functional linkage between ZNF202 and hypoalphalipoproteinemia.

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