scholarly journals The signal peptide-like segment of hpaXm is required for its association to the cell wall in transgenic tobacco plants

PLoS ONE ◽  
2017 ◽  
Vol 12 (1) ◽  
pp. e0170931 ◽  
Author(s):  
Le Li ◽  
Weiguo Miao ◽  
Wenbo Liu ◽  
Shujian Zhang
Keyword(s):  
Cell Wall ◽  
Transgenic Tobacco ◽  
Signal Peptide ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants

scholarly journals Signal Peptide of Potato PinII Enhances the Expression of Cry1Ac in Transgenic Tobacco

2004 ◽  
Vol 36 (8) ◽  
pp. 553-558 ◽  
Author(s):  
Yun-Jun Liu ◽  
Yuan Yuan ◽  
Jun Zheng ◽  
Ya-Zhong Tao ◽  
Zhi-Gang Dong ◽  
...  
Keyword(s):  
Transgenic Tobacco ◽  
Signal Peptide ◽  
Proteinase Inhibitor ◽  
Peptide Sequence ◽  
Fluorescent Detection ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants ◽  
Signal Peptide Sequence ◽  
Proteinase Inhibitor Ii ◽  
Cry1ac Protein

Abstract The modified Cry1Ac was expressed in transgenic tobacco plants. To allow secretion of the Cry1Ac protein into the intercellular space, the signal peptide sequence of potato proteinase inhibitor II (pinII) was N-terminally fused to the Cry1Ac encoding region. Expression of Cry1Ac in transgenic tobacco plants was assayed with ELISA. The results showed that pinII signal peptide sequence enhanced the expression of Cry1Ac protein and led to the secretion of the Cry1Ac protein in transgenic tobacco plants. GFP gene was also fused to the signal peptide sequence and transformed to tobacco. The results of fluorescent detection showed that GFP had localized in the apoplast of transgenic plants.

Reassessment of qualitative changes in lignification of transgenic tobacco plants and their impact on cell wall assembly

2001 ◽  
Vol 57 (6) ◽  
pp. 875-882 ◽  
Author(s):  
Katia Ruel ◽  
Matthieu Chabannes ◽  
Alain-Michel Boudet ◽  
Michel Legrand ◽  
Jean-Paul Joseleau
Keyword(s):  
Cell Wall ◽  
Transgenic Tobacco ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants ◽  
Cell Wall Assembly ◽  
Wall Assembly ◽  
Qualitative Changes

scholarly journals Hevea brasiliensis coniferaldehyde-5-hydroxylase (HbCAld5H) regulates xylogenesis, structure and lignin chemistry of xylem cell wall in Nicotiana tabacum

2020 ◽  
Author(s):  
S. Pramod ◽  
Thakurdas Saha ◽  
K. Rekha ◽  
P. B. Kavi Kishor
Keyword(s):  
Cell Wall ◽  
Transgenic Tobacco ◽  
Hevea Brasiliensis ◽  
Secondary Wall ◽  
Cambial Activity ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants ◽  
Lignin Distribution ◽  
Syringyl Lignin ◽  
S2 Layer

Abstract Key message The HbCAld5H1 gene cloned from Hevea brasiliensis regulates the cambial activity, xylem differentiation, syringyl–guaiacyl ratio, secondary wall structure, lignification pattern and xylan distribution in xylem fibres of transgenic tobacco plants. Abstract Molecular characterization of lignin biosynthesis gene coniferaldehyde-5-hydroxylase (CAld5H) from Hevea brasiliensis and its functional validation was performed. Both sense and antisense constructs of HbCAld5H1 gene were introduced into tobacco through Agrobacterium-mediated genetic transformation for over expression and down-regulation of this key enzyme to understand its role affecting structural and cell wall chemistry. The anatomical studies of transgenic tobacco plants revealed the increase of cambial activity leading to xylogenesis in sense lines and considerable reduction in antisense lines. The ultra-structural studies showed that the thickness of secondary wall (S2 layer) of fibre had been decreased with non-homogenous lignin distribution in antisense lines, while sense lines showed an increase in S2 layer thickness. Maule color reaction revealed that syringyl lignin distribution in the xylem elements was increased in sense and decreased in antisense lines. The immunoelectron microscopy revealed a reduction in LM 10 and LM 11 labelling in the secondary wall of antisense tobacco lines. Biochemical studies showed a radical increase in syringyl lignin in sense lines without any significant change in total lignin content, while S/G ratio decreased considerably in antisense lines. Our results suggest that CAld5H gene plays an important role in xylogenesis stages such as cambial cell division, secondary wall thickness, xylan and syringyl lignin distribution in tobacco. Therefore, CAld5H gene could be considered as a promising target for lignin modification essential for timber quality improvement in rubber.

A hybrid proline-rich cell-wall protein gene JsPRP1 from Juglans sigillata Dode confers both biotic and abiotic stresses in transgenic tobacco plants

Trees ◽  
2018 ◽  
Vol 32 (5) ◽  
pp. 1199-1209 ◽  
Author(s):  
D. Q. Liu ◽  
Q. Han ◽  
T. Shah ◽  
C. Y. Chen ◽  
Q. Wang ◽  
...  
Keyword(s):  
Cell Wall ◽  
Transgenic Tobacco ◽  
Abiotic Stresses ◽  
Protein Gene ◽  
Cell Wall Protein ◽  
Transgenic Tobacco Plants ◽  
Biotic And Abiotic Stresses ◽  
Tobacco Plants ◽  
Juglans Sigillata ◽  
Juglans Sigillata Dode

Production of an active recombinant thrombomodulin derivative in transgenic tobacco plants and suspension cells

2005 ◽  
Vol 14 (3) ◽  
pp. 251-259 ◽  
Author(s):  
Helga Schinkel ◽  
Andreas Schiermeyer ◽  
Raphael Soeur ◽  
Rainer Fischer ◽  
Stefan Schillberg
Keyword(s):  
Transgenic Tobacco ◽  
Transgenic Tobacco Plants ◽  
Suspension Cells ◽  
Tobacco Plants ◽  
Recombinant Thrombomodulin
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