High stringency evaluation of the inactivation / exclusion efficacy of a MALDI-TOF MS chemical extraction method, with filtration of extract through 0.1 µm filters, on Bacillus anthracis Vollum vegetative cells and spores

ABSTRACTThe objective of this study was to construct a rapid, high-throughput, and biosafety-compatible screening method for Bacillus anthracis and Bacillus cereus based on matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS). MALDI-TOF MS coupled to ClinProTools was used to discover MALDI-TOF MS biomarker peaks and generate a classification model based on a genetic algorithm (GA) to differentiate between different Bacillus anthracis and Bacillus cereus isolates. Thirty Bacillus anthracis and 19 Bacillus cereus strains were used to construct and analyze the model, and 40 Bacillus strains were used for validation. For the GA screening model, the cross-validation values, which reflect the ability of the model to handle variability among the test spectra, and the recognition capability values, which reflect the model’s ability to correctly identify its component spectra, were all 100%. This model contained 10 biomarker peaks (m/z 3,339.9, 3,396.3, 3,682.4, 5,476.7, 6,610.6, 6,680.1, 7,365.3, 7,792.4, 9,475.8, and 10,934.1) used to correctly identify 28 Bacillus anthracis and 12 Bacillus cereus isolates from 40 Bacillus isolates, with a sensitivity and specificity of 100%. With the obvious advantages of being rapid, highly accurate, and highly sensitive and having a low cost and high throughput, MALDI-TOF MS ClinProTools is a powerful and reliable tool for screening Bacillus anthracis and Bacillus cereus strains.

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FEATURES OF BACILLUS ANTHRACIS IDENTIFICATION BY MALDI-TOF MS

Russian Journal of Infection and Immunity ◽

10.15789/2220-7619-2018-4-4.13 ◽

2019 ◽

Vol 8 (4) ◽

pp. 546-547

Author(s):

E. A. Koteneva ◽

A. V. Kalinin ◽

O. I. Tsygankova

Keyword(s):

Bacillus Anthracis ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Tof Ms

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Rapid one-step extraction method for the identification of molds using MALDI-TOF MS

Diagnostic Microbiology and Infectious Disease ◽

10.1016/j.diagmicrobio.2018.01.015 ◽

2018 ◽

Vol 91 (2) ◽

pp. 130-135 ◽

Cited By ~ 5

Author(s):

Paul M. Luethy ◽

Adrian M. Zelazny

Keyword(s):

Extraction Method ◽

Maldi Tof Ms ◽

Maldi Tof ◽

One Step ◽

Tof Ms

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STUDY OF THE MASS-SPECTORS’ FEATURES OF SPORES AND VEGETATIVE FORMS OF BACILLUS ANTHRACIS BY THE METHOD OF TIME OF FLIGHT MASS-SPECTROMETRY

Journal of microbiology epidemiology immunobiology ◽

10.36233/0372-9311-2018-6-66-72 ◽

2018 ◽

pp. 66-72

Author(s):

D. V. Ulshina ◽

E. I. Eremenko ◽

D. A. Kovalev ◽

A. G. Ryazanova ◽

I. V. Kuznetsova ◽

...

Keyword(s):

Bacillus Anthracis ◽

Causative Agent ◽

Protein Profiling ◽

Protein Profiles ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Flight Mass Spectrometry ◽

Vegetative Form ◽

Maldi Tof Mass Spectra ◽

Tof Ms

Aim. Investigation of the features of the protein profiles of the spore and vegetative form of the anthrax microbe by the MALDI-TOF MS method using the resources of the Mass-Up program and the package for the statistical software with open source code R. Materials and methods. Spores and vegetative forms of 32 strains of B. anthracis from the collection of microorganisms of the Stavropol Research Institute for Plague Control, including 8 strains isolated from an outbreak of anthrax in Yamal in 2016. Protein profiling was carried out on the Microflex MALDI-TOF mass spectrometer «Bruker Daltonics». Results. The alternative bioinformational-statistical approach used to analyze the MALDI-TOF mass spectra of the causative agent of anthrax made it possible to differentiate the spores and vegetative forms of the microbe based on the identification of the corresponding groups of biomarkers. Conclusion. A comparison of vegetative and spore cells of typical and atypical strains of anthrax causative agent on the basis of MALDI-TOF MS data was made. It has been experimentally confirmed that the protein profiles of cultures of Bacillus anthracis of the spore and vegetative form differ significantly, and this difference can be used to search for potential markers of each of the forms.

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