scholarly journals Cuticular fatty acids of Galleria mellonella (Lepidoptera) inhibit fungal enzymatic activities of pathogenic Conidiobolus coronatus

PLoS ONE ◽  
2018 ◽  
Vol 13 (3) ◽  
pp. e0192715 ◽  
Author(s):  
Anna Katarzyna Wrońska ◽  
Mieczysława Irena Boguś ◽  
Emilia Włóka ◽  
Michalina Kazek ◽  
Agata Kaczmarek ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Galleria Mellonella ◽  
Enzymatic Activities ◽  
Conidiobolus Coronatus

Coronatin-2 from the entomopathogenic fungus Conidiobolus coronatus kills Galleria mellonella larvae and incapacitates hemocytes

2016 ◽  
Vol 107 (1) ◽  
pp. 66-76 ◽  
Author(s):  
M.I. Boguś ◽  
W. Wieloch ◽  
M. Ligęza-Żuber
Keyword(s):  
Entomopathogenic Fungus ◽  
Galleria Mellonella ◽  
Elongation Factor ◽  
Peptide Sequence ◽  
Mass Spectrometry Analysis ◽  
Sequence Coverage ◽  
Spectrometry Analysis ◽  
Conidiobolus Coronatus ◽  
Liquid Chromatography Tandem Mass

AbstractCoronatin-2, a 14.5 kDa protein, was isolated from culture filtrates of the entomopathogenic fungus Conidiobolus coronatus (Costantin) Batko (Entomophthoramycota: Entomophthorales). After LC–MS/MS (liquid chromatography tandem mass spectrometry) analysis of the tryptic peptide digest of coronatin-2 and a mass spectra database search no orthologs of this protein could be found in fungi. The highest homology was observed to the partial translation elongation factor 1a from Sphaerosporium equinum (protein sequence coverage, 21%), with only one peptide sequence, suggesting that coronatin-2 is a novel fungal protein that has not yet been described. In contrast to coronatin-1, an insecticidal 36 kDa protein, which shows both elastolytic and chitinolytic activity, coronatin-2 showed no enzymatic activity. Addition of coronatin-2 into cultures of hemocytes taken from larvae of Galleria mellonella Linnaeus (Lepidoptera: Pyralidae), resulted in progressive disintegration of nets formed by granulocytes and plasmatocytes due to rapid degranulation of granulocytes, extensive vacuolization of plasmatocytes accompanied by cytoplasm expulsion, and cell disintegration. Spherulocytes remained intact, while oenocytes rapidly disintegrated. Coronatin-2 produced 80% mortality when injected into G. mellonella at 5 µg larva−1. Further study is warranted to determine the relevance of the acute toxicity of coronatin-2 and its effects on hemocytes in vitro to virulence of C. coronatus against its hosts.

Metabolism of polyunsaturated fatty acids by larvae of the waxmoth,Galleria mellonella

1987 ◽  
Vol 6 (3) ◽  
pp. 141-149 ◽  
Author(s):  
David W. Stanley-Samuelson ◽  
Russell A. Jurenka ◽  
Werner Loher ◽  
Gary J. Blomquist
Keyword(s):  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Galleria Mellonella

Effects of insect cuticular fatty acids on in vitro growth and pathogenicity of the entomopathogenic fungus Conidiobolus coronatus

2010 ◽  
Vol 125 (4) ◽  
pp. 400-408 ◽  
Author(s):  
Mieczysława I. Boguś ◽  
Maria Czygier ◽  
Marek Gołębiowski ◽  
Elżbieta Kędra ◽  
Jolanta Kucińska ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Entomopathogenic Fungus ◽  
In Vitro Growth ◽  
Conidiobolus Coronatus

Distribution of long-chain fatty acids among the lipid classes of Lucilia sericata (Meigen) and Galleria mellonella (Linnaeus)

1970 ◽  
Vol 48 (4) ◽  
pp. 775-778 ◽  
Author(s):  
O. B. Lindsay ◽  
J. S. Barlow
Keyword(s):  
Fatty Acids ◽  
Palmitoleic Acid ◽  
Galleria Mellonella ◽  
Lucilia Sericata ◽  
Unesterified Fatty Acid ◽  
Long Chain Fatty Acids ◽  
Partial Glyceride ◽  
Greater Wax Moth ◽  
The Difference ◽  
Wax Moth

The larval lipids of the blowfly Lucilia sericata (Meigen) and the greater wax moth Galleria mellonella (Linnaeus), which differ markedly in their palmitoleic acid content, were separated on silicic acid columns into five fractions, viz. (1) cholesterol ester, (2) triglyceride, (3) unesterified fatty acid and cholesterol, (4) partial glyceride, and (5) phospholipid. The fractions were analyzed for individual fatty acids. For each species of insect, palmitoleic acid was found to be distributed among the glyceride and phospholipid fractions in its characteristic proportion in the total fatly acids of that species. Thus the difference in the proportion of palmitoleic acid in the lipids of the two species cannot be attributed to preferential exclusion of palmitoleic acid from the triglycerides of Galleria mellonella. In Lucilia sericata the principal fatty acids are partitioned among the glyceride and phospholipid fractions to virtually the same degree, but in Galleria mellonella they are partitioned unevenly. This unevenness in partitioning is most evident with linoleic acid, which is relatively high in the phospholipids.

Changes in enzymatic activities involved in glucose metabolism by acyl-CoAs in Trypanosoma cruzi

2001 ◽  
Vol 47 (1) ◽  
pp. 49-54 ◽  
Author(s):  
M García de Lema ◽  
G Lucchesi ◽  
G Racagni ◽  
E E Machado-Domenech
Keyword(s):  
Fatty Acids ◽  
Glucose Metabolism ◽  
Trypanosoma Cruzi ◽  
Free Fatty Acids ◽  
Chain Length ◽  
Acid Concentration ◽  
Enzymatic Activities ◽  
Coa Thioesters ◽  
Glucose 6 Phosphate Dehydrogenase

This study describes the effect of some saturated and unsaturated free fatty acids and acyl-CoA thioesters on Trypanosoma cruzi glucose 6-phosphate dehydrogenase and hexokinase activities. Glucose 6-phosphate dehydrogenase was sensitive to the destabilizing effect provoked by free fatty acids, while hexokinase remained unaltered. Glucose 6-phosphate dehydrogenase inhibition by free fatty acids was dependent on acid concentration and chain length. Both enzymes were inhibited when they were incubated with acyl-CoA thioesters. The acyl-CoA thioesters inhibited glucose 6-phosphate dehydrogenase at a lower concentration than the free fatty acids; the ligands glucose 6-phosphate and NADP+ afforded protection. The inhibition of hexokinase by acyl-CoAs was not reverted when the enzyme was incubated with ATP. The type of inhibition found with acyl-CoAs in relation to glucose 6-phosphate dehydrogenase and hexokinase suggests that this type inhibition may produce an in vivo modulation of these enzymatic activities.Key words: Trypanosoma cruzi, fatty acids, acyl-CoAs, glucose 6-phosphate dehydrogenase, hexokinase.

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