scholarly journals DNA barcoding of southern African crustaceans reveals a mix of invasive species and potential cryptic diversity

PLoS ONE ◽  
2019 ◽  
Vol 14 (9) ◽  
pp. e0222047 ◽  
Author(s):  
Bezeng S. Bezeng ◽  
Herman F. van der Bank
2010 ◽  
Vol 13 (6) ◽  
pp. 1325-1340 ◽  
Author(s):  
Elizabeta Briski ◽  
Melania E. Cristescu ◽  
Sarah A. Bailey ◽  
Hugh J. MacIsaac

2021 ◽  
Vol 5 (5) ◽  
Author(s):  
Juha Salokannel ◽  
Kyung Min Lee ◽  
Aki Rinne ◽  
Marko Mutanen

Abstract Large-scale global efforts on DNA barcoding have repeatedly revealed unexpected patterns of variability in mtDNA, including deep intraspecific divergences and haplotype sharing between species. Understanding the evolutionary causes behind these patterns calls for insights from the nuclear genome. While building a near-complete DNA barcode library of Finnish caddisflies, a case of barcode-sharing and some cases of deep intraspecific divergences were observed. In this study, the Apatania zonella (Zetterstedt, 1840) group and three Limnephilus Leach, 1815 species were studied using double digest RAD sequencing (ddRAD-seq), morphology, and DNA barcoding. The results support the present species boundaries in the A. zonella group species. A morphologically distinct but mitogenetically nondistinct taxon related to parthenogenetic Apatania hispida (Forsslund, 1930) got only weak support for its validity as a distinct species. The morphology and genomic-scale data do not indicate cryptic diversity in any of the three Limnephilus species despite the observed deep intraspecific divergences in DNA barcodes. This demonstrates that polymorphism in mtDNA may not reflect cryptic diversity, but mitonuclear discordance due to other evolutionary causes.


2010 ◽  
pp. 289-299 ◽  
Author(s):  
Hugh B. Cross ◽  
Andrew J. Lowe ◽  
Frederico D. Gurgel

2013 ◽  
Vol 13 (4) ◽  
pp. 596-606 ◽  
Author(s):  
Te‐Hua Hsu ◽  
Yue Ning ◽  
Jin‐Chywan Gwo ◽  
Zhi‐Nan Zeng

2013 ◽  
Vol 86 ◽  
pp. 175
Author(s):  
D.M. Komape ◽  
L.I. Mabe ◽  
S.J. Siebert ◽  
M. Struwig ◽  
T.M. Sethusa ◽  
...  

Nematology ◽  
2014 ◽  
Vol 16 (8) ◽  
pp. 979-989 ◽  
Author(s):  
Maickel Armenteros ◽  
Ariadna Rojas-Corzo ◽  
Alexei Ruiz-Abierno ◽  
Sofie Derycke ◽  
Thierry Backeljau ◽  
...  

The diversity and phylogenetic relationships of the Desmodoridae, a widespread tropical family of free-living marine nematodes, is hitherto poorly known both from molecular and taxonomic points of view. We performed a molecular phylogenetic analysis of marine nematodes to: i) disentangle relationships among tropical desmodorid species; and ii) compare the performance of the nuclear SSU rDNA and mitochondrial COI nucleotide sequences in 42 and 45 nominal species, respectively, to identify species. We generated 27 new sequences of SSU rDNA belonging to five genera not previously sequenced, and 34 new sequences of COI belonging to six genera and four families not previously sequenced. The SSU rDNA tree confirmed the Enoplida to be a monophyletic sister group to the Chromadorida. The family Comesomatidae is a sister group of the Xyalidae within the Monhysterida. Both DNA markers confirmed the congruence between the morphology- and molecular-based phylogenetic inferences for most of the families. Desmodoridae was a monophyletic group, but the relationships within the family could not be recovered; the subfamilies Desmodorinae and Spiriniinae were not monophyletic meanwhile the monophyly of Stilbonematinae was not fully supported due to a few specimens of questionable identity. COI performed better than SSU rDNA to disentangle relationships among closely related species and suggested the presence of cryptic diversity within Desmodoridae. COI is effective to explore cryptic diversity and barcode species within Nematoda, with a possible threshold of genetic distance of 5% between conspecific and interspecific sequences, but DNA barcoding is limited by the poor knowledge of the diversity and taxonomy of the group and the lack of a good reference database of vouchered COI sequences.


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