Use of DNA barcoding to detect invertebrate invasive species from diapausing eggs

Abstract In this work, we identified non-crustose invasive (Codium fragile subsp. fragile) and native Codium spp. (Codium tomentosum and Codium vermilara) in the central Cantabrian Sea using DNA barcoding (tufA and rbcL genes). We designed a new FCOtufA genetic marker for identifying Codium spp. in fresh and herbarium material. The tufA and rbcL sequences revealed three different single haplotypes for each of the species and a lack of intraspecific genetic diversity. The FCOtufA genetic marker revealed one new haplotype of C. fragile within the native region (South Korea), suggesting the possibility of higher genetic diversity in the donor region of this invasive species.

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DNA barcoding invasive insects: database roadblocks

Invertebrate Systematics ◽

10.1071/is12025 ◽

2012 ◽

Vol 26 (6) ◽

pp. 506 ◽

Cited By ~ 18

Author(s):

Laura M. Boykin ◽

Karen Armstrong ◽

Laura Kubatko ◽

Paul De Barro

Keyword(s):

Invasive Species ◽

Missing Data ◽

Dna Barcoding ◽

Genetic Data ◽

Invasive Insects ◽

Identification Number ◽

Use Of Resources ◽

Private Data ◽

Parallel Data ◽

Data Coverage

This study examines the genetic data coverage and availability in the Barcode of Life Database (BOLD), versions 2.5 and 3.0, and GenBank for the 88 invasive insects listed in the Global Invasive Species Database (http://www.issg.org). No data are recorded in either BOLD or GenBank for seven of those species. As a dedicated repository of curated barcode data BOLD is either missing data or contains inaccessible private data for 37 (42%) of the species while no data are available in GenBank for nine (8%) of the species. An evaluation of the Barcode Identification Number (BIN) scheme in BOLD ver. 3.0 was also evaluated and in 41% of cases the BIN contained more than one species. This essentially arose due to the 1% delimitation thresholds associated with the BINs and would result in misidentifications. Overall, more information is available from GenBank for the 88 invasive species listed on the Global Invasive Species Database, but quality checking is required to ensure that the data extracted from GenBank are of sufficient quality to make it useful. The implications of these results are discussed, with investment in parallel data silos suggested to be both costly and potentially an inefficient use of resources that may lead to loss of data if the means needed to maintain these databases become unavailable.

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DNA barcoding of southern African crustaceans reveals a mix of invasive species and potential cryptic diversity

PLoS ONE ◽

10.1371/journal.pone.0222047 ◽

2019 ◽

Vol 14 (9) ◽

pp. e0222047 ◽

Cited By ~ 4

Author(s):

Bezeng S. Bezeng ◽

Herman F. van der Bank

Keyword(s):

Invasive Species ◽

Dna Barcoding ◽

Cryptic Diversity

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DNA barcoding of Greenideinae (Hemiptera : Aphididae) with resolving taxonomy problems

Invertebrate Systematics ◽

10.1071/is13014 ◽

2013 ◽

Vol 27 (4) ◽

pp. 428 ◽

Cited By ~ 11

Author(s):

Qing-Hua Liu ◽

Li-Yun Jiang ◽

Ge-Xia Qiao

Keyword(s):

Invasive Species ◽

Dna Barcoding ◽

Morphological Traits ◽

Mitochondrial Gene ◽

Morphological Evidence ◽

South East Asia ◽

Cytb Gene ◽

Gene Fragment ◽

Congeneric Species ◽

Species Circumscription

Species of the Greenideinae are distributed mainly throughout South-east Asia and include some important agricultural and horticultural pests. Rapid and accurate species circumscription and identification in this subfamily are very difficult because similar morphological traits are shared among congeneric species. Here, we test the efficiency of DNA barcoding in the Greenideinae by analysing 214 samples covering 42 species belonging to nine genera using two mitochondrial gene fragments (COI barcode fragment and Cytb gene fragment). The results show that DNA barcoding is a useful species identification method in this subfamily. Both genes can correctly identify most species using neighbour-joining tree analyses and distance-based analyses. Based on the molecular and morphological evidence, we question the validity of two species, Mollitrichosiphum rhusae Ghosh, 1917 and Schoutedenia emblica (Patel & Kulkarni, 1953). Further analysis of the COI barcode fragment shows that Greenidea psidii van der Goot, 1917, an invasive species in Hawaii, is possibly from China. This is a preliminary DNA barcoding study in Greenideinae, and comprehensive sampling is needed to rigorously test the usefulness of DNA barcoding in this subfamily.

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DNA Barcoding: A Potential Tool for Invasive Species Identification

DNA Barcoding and Molecular Phylogeny ◽

10.1007/978-3-319-90680-5_5 ◽

2018 ◽

pp. 73-85 ◽

Cited By ~ 1

Author(s):

Muniyandi Nagarajan ◽

Akash Nambidi Parambath ◽

Vandana R. Prabhu

Keyword(s):

Invasive Species ◽

Dna Barcoding ◽

Species Identification ◽

Potential Tool

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DNA barcoding of British mosquitoes (Diptera, Culicidae) to support species identification, discovery of cryptic genetic diversity and monitoring invasive species

ZooKeys ◽

10.3897/zookeys.832.32257 ◽

2019 ◽

Vol 832 ◽

pp. 57-76 ◽

Cited By ~ 14

Author(s):

Luis M. Hernández-Triana ◽

Victor A. Brugman ◽

Nadya I. Nikolova ◽

Ignacio Ruiz-Arrondo ◽

Elsa Barrero ◽

...

Keyword(s):

Invasive Species ◽

Dna Barcoding ◽

Species Identification ◽

Mosquito Species ◽

Extraction Methods ◽

Control Programs ◽

Species Groups ◽

Dna Extraction Methods ◽

The Uk ◽

Cryptic Genetic Diversity

Correct mosquito species identification is essential for mosquito and disease control programs. However, this is complicated by the difficulties in morphologically identifying some mosquito species. In this study, variation of a partial sequence of the cytochromecoxidase unit I (COI) gene was used for the molecular identification of British mosquito species and to facilitate the discovery of cryptic diversity, and monitoring invasive species. Three DNA extraction methods were compared to obtain DNA barcodes from adult specimens. In total, we analyzed 42 species belonging to the generaAedesMeigen, 1818 (21 species),AnophelesMeigen, 1818 (7 species),CoquillettidiaTheobald, 1904 (1 species),CulexLinnaeus, 1758 (6 species),CulisetaFelt, 1904 (7 species), andOrthopodomyiaTheobald, 1904 (1 species). Intraspecific genetic divergence ranged from 0% to 5.4%, while higher interspecific divergences were identified betweenAedesgeminusPeus, 1971/Culisetalitorea(Shute, 1928) (24.6%) andAe.geminus/An.plumbeusStephens, 1828 (22.5%). Taxonomic discrepancy was shown betweenAn.daciaeLinton, Nicolescu & Harbach, 2004 andAn.messeaeFalleroni, 1828 indicating the poor resolution of theCOIDNA barcoding region in separating these taxa. Other species such asAe.cantans(Meigen, 1818)/Ae.annulipes(Meigen, 1830) showed similar discrepancies indicating some limitation of this genetic marker to identify certain mosquito species. The combination of morphology and DNA barcoding is an effective approach for the identification of British mosquitoes, for invasive mosquitoes posing a threat to the UK, and for the detection of hidden diversity within species groups.

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