scholarly journals New label-free methods for protein relative quantification applied to the investigation of an animal model of Huntington Disease

PLoS ONE ◽  
2020 ◽  
Vol 15 (9) ◽  
pp. e0238037
Author(s):  
Flora Cozzolino ◽  
Alfredo Landolfi ◽  
Ilaria Iacobucci ◽  
Vittoria Monaco ◽  
Marianna Caterino ◽  
...  
Keyword(s):  
Animal Model ◽  
Huntington Disease ◽  
Relative Quantification ◽  
Label Free

scholarly journals Narrowband Modulation Two-Dimensional Mass Spectrometry and Label-Free Relative Quantification of Histone Peptides

2020 ◽  
Vol 92 (20) ◽  
pp. 13945-13952
Author(s):  
Matthias Halper ◽  
Marc-André Delsuc ◽  
Kathrin Breuker ◽  
Maria A. van Agthoven
Keyword(s):  
Mass Spectrometry ◽  
Relative Quantification ◽  
Two Dimensional ◽  
Label Free ◽  
Free Relative

scholarly journals Label-free relative quantification of alpha-2-macroglobulin site-specific core-fucosylation in pancreatic cancer by LC-MS/MS

2013 ◽  
pp. n/a-n/a ◽  
Author(s):  
Zhenxin Lin ◽  
Haidi Yin ◽  
Andy Lo ◽  
Mack T. Ruffin ◽  
Michelle A. Anderson ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Relative Quantification ◽  
Label Free ◽  
Site Specific ◽  
Free Relative ◽  
Core Fucosylation

scholarly journals Development and Evaluation of Normalization Methods for Label-free Relative Quantification of Endogenous Peptides

2009 ◽  
Vol 8 (10) ◽  
pp. 2285-2295 ◽  
Author(s):  
Kim Kultima ◽  
Anna Nilsson ◽  
Birger Scholz ◽  
Uwe L. Rossbach ◽  
Maria Fälth ◽  
...  
Keyword(s):  
Relative Quantification ◽  
Label Free ◽  
Endogenous Peptides ◽  
Normalization Methods ◽  
Free Relative

Comprehensive Label-Free Method for the Relative Quantification of Proteins from Biological Samples

2005 ◽  
Vol 4 (4) ◽  
pp. 1442-1450 ◽  
Author(s):  
Richard E. Higgs ◽  
Michael D. Knierman ◽  
Valentina Gelfanova ◽  
Jon P. Butler ◽  
John E. Hale
Keyword(s):  
Biological Samples ◽  
Relative Quantification ◽  
Label Free

Strategies in relative and absolute quantitative mass spectrometry based proteomics

2017 ◽  
Vol 398 (5-6) ◽  
pp. 687-699 ◽  
Author(s):  
Claudia Lindemann ◽  
Nikolas Thomanek ◽  
Franziska Hundt ◽  
Thilo Lerari ◽  
Helmut E. Meyer ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Experimental Studies ◽  
Olfactory Receptors ◽  
Absolute Quantification ◽  
Relative Quantification ◽  
Label Free ◽  
Quantitative Mass Spectrometry ◽  
Proteome Coverage ◽  
Sensory Cilia ◽  
Core Components

Abstract Quantitative mass spectrometry approaches are used for absolute and relative quantification in global proteome studies. To date, relative and absolute quantification techniques are available that differ in quantification accuracy, proteome coverage, complexity and robustness. This review focuses on most common relative or absolute quantification strategies exemplified by three experimental studies. A label-free relative quantification approach was performed for the investigation of the membrane proteome of sensory cilia to the depth of olfactory receptors in Mus musculus. A SILAC-based relative quantification approach was successfully applied for the identification of core components and transient interactors of the peroxisomal importomer in Saccharomyces cerevisiae. Furthermore, AQUA using stable isotopes was exemplified to unraveling the prenylome influenced by novel prenyltransferase inhibitors. Characteristic enrichment and fragmentation strategies for a robust quantification of the prenylome are also summarized.

scholarly journals Label-free relative quantification applied to LC-MALDI acquisition for rapid analysis of chondrocyte secretion modulation

2015 ◽  
Vol 114 ◽  
pp. 263-273 ◽  
Author(s):  
Mathieu Riffault ◽  
David Moulin ◽  
Laurent Grossin ◽  
Didier Mainard ◽  
Jacques Magdalou ◽  
...  
Keyword(s):  
Rapid Analysis ◽  
Relative Quantification ◽  
Label Free ◽  
Free Relative

Label-free mass spectrometry-based relative quantification of proteins separated by one-dimensional gel electrophoresis

2011 ◽  
Vol 409 (2) ◽  
pp. 202-212 ◽  
Author(s):  
Melkamu Getie-Kebtie ◽  
Alexander Lazarev ◽  
Maryna Eichelberger ◽  
Michail Alterman
Keyword(s):  
Mass Spectrometry ◽  
Gel Electrophoresis ◽  
Relative Quantification ◽  
Label Free ◽  
One Dimensional
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