The Use of Hippocampal Slices for the Study of Energy Metabolism

2003 ◽  
pp. 99-132 ◽  
Author(s):  
Tim S. Whittingham
Keyword(s):  
Energy Metabolism ◽  
Hippocampal Slices

Energy metabolism and neural activity during deprivation of oxygen and/or glucose in the hippocampal slices of immature and adult rat

1992 ◽  
Vol 17 ◽  
pp. 69
Author(s):  
Makoto Nabetani ◽  
Yukihiro Yamada ◽  
Shinji Kawai ◽  
Masahiko Yonetani ◽  
Satoshi Takada ◽  
...  
Keyword(s):  
Energy Metabolism ◽  
Neural Activity ◽  
Hippocampal Slices ◽  
Adult Rat

scholarly journals Seizures and Amyloid-β induce similar changes in neuronal network metabolic parameters in mouse hippocampal slices

2016 ◽  
Author(s):  
Anton Ivanov ◽  
Anton E Malkov ◽  
Svetlna Buldakova ◽  
Misha Zilberter ◽  
Yuri Zilberter
Keyword(s):  
Energy Metabolism ◽  
Neurodegenerative Diseases ◽  
Energy State ◽  
Hippocampal Slices ◽  
Amyloid Β ◽  
Epileptic Seizures ◽  
Common Path ◽  
Network Activation ◽  
Extracellular Glucose ◽  
Neuronal Energy Metabolism

Major risk factors for neurodegenerative diseases share brain hypometabolism as one common outcome. In turn, many neurodegenerative pathologies result in brain hypometabolism; both epilepsy and Alzheimer's disease are characterised by disruptions in glucose metabolism. However, the causative link between energy shortage and neuronal pathologies in these disease has remained elusive. Using real-time brain slice recordings of energy metabolism parameter (NAD(P)H, FAD, pO2 and extracellular glucose) transients in response to network activation, we found that induced epileptic seizures and amyloid-beta peptide both result in similar and long-lasting disruptions of neuronal energy metabolism, suggesting a common path of action. In addition, we found that in both cases, subsequent addition of pyruvate, the principal mitochondrial fuel possessing multiple neuroprotective properties, completely normalised the disputed energy state. Our data suggests that energy metabolism disruptions underlie the initiation and progression of neurodegenerative diseases.

scholarly journals Glycolysis and Oxidative Phosphorylation in Neurons and Astrocytes during Network Activity in Hippocampal Slices

2013 ◽  
Vol 34 (3) ◽  
pp. 397-407 ◽  
Author(s):  
Anton I Ivanov ◽  
Anton E Malkov ◽  
Tatsiana Waseem ◽  
Marat Mukhtarov ◽  
Svetlana Buldakova ◽  
...  
Keyword(s):  
Energy Metabolism ◽  
Oxidative Phosphorylation ◽  
Hippocampal Slices ◽  
Network Activity ◽  
Oxygen Species ◽  
Energy Substrate ◽  
Cellular Origin ◽  
Network Activation ◽  
Energy Demands ◽  
Artificial Cerebrospinal Fluid

Network activation triggers a significant energy metabolism increase in both neurons and astrocytes. Questions of the primary neuronal energy substrate (e.g., glucose vs. lactate) as well as the relative contributions of glycolysis and oxidative phosphorylation and their cellular origin (neurons vs. astrocytes) are still a matter of debates. Using simultaneous measurements of electrophysiological and metabolic parameters during synaptic stimulation in hippocampal slices from mature mice, we show that neurons and astrocytes use both glycolysis and oxidative phosphorylation to meet their energy demands. Supplementation or replacement of glucose in artificial cerebrospinal fluid (ACSF) with pyruvate or lactate strongly modifies parameters related to network activity-triggered energy metabolism. These effects are not induced by changes in ATP content, pHi, [Ca2+]i or accumulation of reactive oxygen species. Our results suggest that during network activation, a significant fraction of NAD(P)H response (its overshoot phase) corresponds to glycolysis and the changes in cytosolic NAD(P)H and mitochondrial FAD are coupled. Our data do not support the hypothesis of a preferential utilization of astrocyte-released lactate by neurons during network activation in slices—instead, we show that during such activity glucose is an effective energy substrate for both neurons and astrocytes.

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